Formulation and Evaluation of Phytosomal Gel of Hesperidin
Abstract
Background: Hesperidin possesses potent antioxidant and anti-inflammatory activity but exhibits limited topical efficacy due to poor solubility and low skin permeability. Phytosomal systems can enhance dermal delivery of such phytoconstituents.
Materials and Methods: Hesperidin–phospholipid phytosomes were prepared by the thin-film hydration method and optimized based on particle size, zeta potential, and entrapment efficiency. The optimized formulation (F6) was incorporated into a Carbopol 934 gel and evaluated for physicochemical properties. In-vitro diffusion studies were performed using Franz diffusion cells, while FTIR analysis and release kinetics were also assessed.
Results: The optimized phytosomes showed nanosized vesicles (168 nm), stable zeta potential, and high entrapment efficiency (85.52%). The phytosomal gel exhibited acceptable physicochemical characteristics and sustained drug release, achieving 95.6% release within 8 h. FTIR confirmed compatibility, and drug release followed the Higuchi model (R² = 0.985).
Conclusion: The hesperidin phytosomal gel enhanced solubility, skin permeation, and sustained drug release, demonstrating its potential for effective topical antioxidant and anti-inflammatory therapy.
Keywords: Hesperidin; Phospholipid; Thin-film hydration method; Phytosomes; Topical gel; In-vitro drug release studies.
Keywords:
Hesperidin, Phospholipid, Thin-film hydration method, Phytosomes, Topical gel, In-vitro drug release studiesDOI
https://doi.org/10.22270/jddt.v16i1.7510References
1. Cui L, Zhang Z, Sun Y, Jiang J, Development and evaluation of hesperidin-loaded nanocarriers for improved topical delivery, International Journal of Pharmaceutics, 2020; 586:119577.
2. Goyal A, Sharma P, Ramawat KG, Phytochemicals of citrus species: A review, Plant Science Today, 2017; 4(3):115-123.
3. Kumar S, Mishra A, Hesperidin bioavailability enhancement strategies: An overview, Journal of Drug Delivery and Therapeutics, 2019; 9(3):660-666.
4. Basu S, Ghosh A, Phytosomes as novel drug delivery systems for enhanced delivery of herbal extracts and phytoconstituents, Journal of Drug Delivery Science and Technology, 2019; 52:15-25.
5. Bombardelli E, Mustich G, Phytosome: A new herbal drug delivery system, Fitoterapia, 1991; 62(1):15-22.
6. Pingali PS, Srinivas P, Reddy BM, Miconazole-loaded novel phytosomal topical gels, World Journal of Pharmacy and Pharmaceutical Sciences, 2015; 4(10):2305-2320.
7. Charyulu NR, Joshi P, Dubey A, Shetty A, Emulgel: A boon for enhanced topical drug delivery, Journal of Young Pharmacists, 2021; 13(1):76-79. https://doi.org/10.5530/jyp.2021.13.17
8. Singh R, Narke R, Preparation and evaluation of phytosome of lawsone, International Journal of Pharmaceutical Sciences and Research, 2015; 6(12):5217-5226.
9. Sayad IW, Zaheer Z, Validated UV spectrophotometric method for estimation of bifonazole in bulk drug and cream pharmaceutical formulation, Inventi Rapid: Pharm Analysis and Quality Assurance, 2013; 1(3):1-6.
10. Singh RP, Narke R, Preparation and evaluation of phytosome of lawsone, International Journal of Pharmaceutical Sciences and Research, 2015; 6(12):5217-5226.
11. Das MK, Kalita B, Design and evaluation of phyto-phospholipid complexes (phytosomes) of rutin for transdermal application, Journal of Applied Pharmaceutical Science, 2014; 4(10):051-057. https://doi.org/10.7324/JAPS.2014.401010
12. Nikalje AP, Tiwari S, Ethosomes: A novel tool for transdermal drug delivery, International Journal of Research in Pharmaceutical Sciences, 2012; 2(1):1-20.
13. Honeywell-Nguyen PL, Bouwstra JA, Vesicles as a tool for transdermal and dermal delivery, Drug Discovery Today: Technologies, 2005; 2:67-74. https://doi.org/10.1016/j.ddtec.2005.05.003 PMid:24981757
14. Joshua JM, Anilkumar A, CU V, Vasudevan DT, Surendran SA, Formulation and evaluation of antiaging phytosomal gel, Asian Journal of Pharmaceutical and Clinical Research, 2018; 11(3):1-5. https://doi.org/10.22159/ajpcr.2018.v11i3.24257
15. Rajashekar K, Prasuna Sundari PJ, Srinivas P, Development of a topical phytosomal gel of Woodfordia fruticosa, World Journal of Pharmacy and Pharmaceutical Sciences, 2015; 4(11):919-931.
16. Singh RP, Narke R, Preparation and evaluation of phytosome of lawsone, International Journal of Pharmaceutical Sciences and Research, 2015; 6(12):5217-5225.
17. Rajashekhar K, Sundari P, Srinivas P, Development of a topical phytosomal gel of Woodfordia fruticosa, World Journal of Pharmacy and Pharmaceutical Sciences, 2010; 4:919-932.
18. Bombardelli E, Curri SB, Loggia RD, Del NP, Tubaro A, Complexes between phospholipids and vegetal derivatives of biological interest, Fitoterapia, 1989; 60(1):1-9.
19. Pingali PS, Srinivas P, Reddy BM, Formulation and evaluation of phytosomal drug delivery system for topical application, World Journal of Pharmacy and Pharmaceutical Sciences, 2015; 4(9):1200-1212.
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