SILVER NANOPARTICLES OF MORINGA OLEIFERA â€“ GREEN SYNTHESIS,CHARACTERISATION AND ITS ANTIMICROBIAL EFFICACY
Abstract : The traditional medicinal plant Moringa oleifera is an integral part of the Indian diet and has notable beneficial effects in its leaves, stems, flowers, roots, bark and seeds. It has reported properties like antimicrobial, anti inflammatory, ant diabetic, anti oxidative, anti tumorogenic amongst many other properties. In the present study we have devised a new green method of synthesis of silver nanoparticles to evaluate its antimicrobial efficacy using the aqueous plant extract as the reductant as well as the stabilizer. A cold method of synthesis of silver nanoparticles using silver nitrate solution is performed.
After the synthesis step, the nanoparticles are characterised using UV VIS spectroscopy, scanning electron microscopy and transmission electron microscopy. The results show the incorporation of the silver ions in the extract and also the reduction of the particle size to the nano range.
The antimicrobial potential of these nanoparticles synthesized is tested against various gram positive and gram negative strains of bacteria keeping streptomycin as the standard positive control antibiotic. The antibiotic assay is performed using agar well diffusion method and comparable results are obtained in comparison to the standard antibiotic.
It is seen that the nanoparticles have good antibacterial efficacy against the tested strains. Hence nanoparticles of Moringa oleifera aqueous extracts can be used as a potential alternative to traditional antibiotics using this non toxic safe way of green synthesis.
Keywords: Moringa oleifera, silver nanoparticles, antimicrobial
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