Physicochemical and microbiological characteristics of Calotropis procera Ait. root bark powders from 20 sites in Burkina Faso for standardization
Abstract
Calotropis procera is a component of the phytomedicine FACA®, used to manage sickle cell disease in Burkina Faso. This work aimed to standardize plant powders by evaluating the physicochemical characteristics and microbial quality of Calotropis procera root bark powders from different sites, with properties similar to those of the reference harvest site, to optimize the production of the phytomedicine. The samples were collected from different geographical areas of Burkina Faso during the dry season. The population, physicochemical characteristics, and microbial quality of each powder were evaluated. Twenty (20) geolocated harvest sites were identified with various soil types (clay, silt, gravel, and sand). The availability of Calotropis procera was high in Ziniaré, Ouaga 1, and Boromo; average across 8 sites; and low across 9. Physicochemical analyses of the powders revealed general uniformity in pH and residual moisture content. However, differences were observed in color, bitterness, and hygroscopicity. Total phenolics could serve as tracers, given their content is comparable to that of the reference. All samples complied with the specifications of the European Pharmacopeia 11th edition for total aerobic bacteria, yeasts and molds, and E. coli. Pathogens such as Salmonella and Staphylococcus aureus have been found in some powders. This study identified the optimal sites for collecting Calotropis procera samples.
Keywords: Sickle cell disease; Calotropis procera; root bark; geographical variability.
Keywords:
sickle cell disease, Calotropis procera, root bark, geographical variabilityDOI
https://doi.org/10.22270/jddt.v16i8.7905References
1. OMS, Stratégie de l’OMS pour la médecine traditionnelle pour 2014-2023. Organisation mondiale de la Santé: 2013.
2. Traoré, G. H.; Sanou, L.; Koala, J., Diversité d’utilisations et de connaissances des espèces locales préférées dans le corridor forestier de la Boucle du Mouhoun, Burkina Faso. Sciences naturelles et appliquées 2019, 38 (1), 101-117.
3. Ouoba, K. Contribution à l’amélioration du cadre réglementaire, institutionnel et opérationnel de la pharmacovigilance des médicaments traditionnels au Burkina Faso et dans les pays membres de l’UEMOA. Université de Strasbourg; Université Joseph Ki-Zerbo (Ouagadougou, Burkina Faso), 2022.
4. Direction.Générale.de.l’Accès.aux.Produits.de.Santé, Plan de développement de la production pharmaceutique nationale 2026-2030. Ministère de la Santé, 2025; p 141.
5. OMS L’Organisation mondiale de la Santé encourage les pays de la Région africaine à promouvoir des médicaments traditionnels sûrs et efficaces; 2020.
6. Ouedraogo, S.; Yoda, J.; Traore, T. K.; Nitiema, M.; Sombie, B. C.; Diawara, H. Z.; Yameogo, J. B.; Djande, A.; Belemnaba, L.; Kini, F. B., Production de matières premières et fabrication des médicaments à base de plantes médicinales. International Journal of Biological and Chemical Sciences 2021, 15 (2), 750-772. https://doi.org/10.4314/ijbcs.v15i2.28
7. Ouédraogo, S.; Sombié, B. C.; Ouédraogo, J. C. W.; Traoré, T. K.; Traoré, S.; Nitiéma, M.; Lazare, B.; Sylvin, O.; Rasmané, S.; Pierre, G. I., Standardization of Extracts from Trunks’s Barks of Lannea microcarpa engl. and K. Krause (Anacardiaceae) and Anogeissus leiocarpus (DC) Guill. and Perr.(Combretaceae) for the Formulation of Antihypertensive Herbal Medicines.”. Int. J. Pharm. Sci. Rev. Res 2018, 48 (1), 92-97.
8. Ouédraogo, J. C. W.; Kini, F. B.; Belemnaba, L.; Ouédraogo, S.; Guissou, I. P., Standardisation de la poudre des écorces de racine de Zanthoxylum zanthoxyloides en vue la production d’un phytomédicament anti-drépanocytaire pédiatrique/Standardization of bark root powder of Zanthoxylum zanthoxyloides for the production of pediatric phytomedecine against Sickle Cell Disease. Journal de la Société Ouest-Africaine de Chimie 2015, 40, 24.
9. Pant, P.; Pandey, S.; Dall’Acqua, S., The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. Chemistry & biodiversity 2021, 18 (11), e2100345. https://doi.org/10.1002/cbdv.202100345 PMid:34533273
10. Xu, W.; Cheng, Y.; Guo, Y.; Yao, W.; Qian, H., Effects of geographical location and environmental factors on metabolite content and immune activity of Echinacea purpurea in China based on metabolomics analysis. Industrial Crops and Products 2022, 189, 115782. https://doi.org/10.1016/j.indcrop.2022.115782
11. Gandhi, Y.; Mishra, S. K.; Kumar, V.; Rawat, H.; Kumar, R.; Singh, R.; Singh, A.; Narasimhaji, C. V.; Srikanth, N.; Acharya, R., Effects of geographical variation on the phytochemicals gallic acid, corilagin, and ellagic acid, as well as medicinal properties of Emblica officinalis Gaertn (Fruit). Food and Humanity 2024, 3, 100372. https://doi.org/10.1016/j.foohum.2024.100372
12. Kunle, O. F.; Egharevba, H. O.; Ahmadu, P. O., Standardization of herbal medicines-A review. International Journal of Biodiversity and Conservation 2012, 4 (3), 101-112. https://doi.org/10.5897/IJBC11.163
13. Ouédraogo, S.; Traoré, T. K.; Atchade, B. C.; Kaboré, T.; Ouédraogo, N.; Semdé, R., Standardisation and physicochemical characterisation of trunk bark extracts of Parkia biglobosa used in the manufacture of plant-based medicines. International Journal of Pharmaceutical Research 2024, 16 (4), 11. https://doi.org/10.31838/ijpr/2024.16.04.003
14. Pharmacopée.africaine, P.A. CSTR/OUA. Lagos (Nigeria) ed.; 1985; Vol. 1, p 264 pages
15. Ouédraogo, M. Etude pharmacochimique du macéré aqueux des écorces de racines de Calotropis procera Ait. utilisées en phytothérapie de la maladie drépanocytaire au Burkina Faso. Thèse doctorat de pharmacie, UFR/SDS université d’Ouagadougou, 89p, 2001.
16. Ph.Eur., Pharmacopée européenne 11e édition. EDQM (Conseil de l’Europe). 11e ed. ed.; 2023.
17. Wagner, H.; Bladt, S., Plant drug analysis: a thin layer chromatography atlas. Springer Science & Business Media: 1996. https://doi.org/10.1007/978-3-642-00574-9
18. Singleton, V. L.; Orthofer, R.; Lamuela-Raventos, R. M., Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in enzymology 1999, 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
19. Dogara, A. M., A systematic review on the biological evaluation of Calotropis procera (Aiton) Dryand. Future Journal of Pharmaceutical Sciences 2023, 9 (1), 16. https://doi.org/10.1186/s43094-023-00467-3
20. Bhardwaj, G. S.; Jain, A.; Jangid, T.; Jangir, R. N.; Sharma, A., Calotropis procera: A comprehensive review of its phytochemistry, ethnomedicinal uses, and pharmacological potential. South African Journal of Botany 2025, 185, 235-276. https://doi.org/10.1016/j.sajb.2025.08.001
21. Kaur, A.; Batish, D. R.; Kaur, S.; Chauhan, B. S., An Overview of the Characteristics and Potential of Calotropis procera From Botanical, Ecological, and Economic Perspectives. Frontiers in plant science 2021, 12, 690806. https://doi.org/10.3389/fpls.2021.690806 PMid:34220914 PMCid:PMC8248367
22. Niang, K.; Sagna, M.; Ndiaye, O.; Thiaw, A.; Diallo, A.; Akpo, L. E.; Saleh, M. M.; Diome, N.; Diatta, S.; Faye, M., Analyse de la disponibilité des arbres et d e leurs usages dans la région du Ferlo sénégalais: les fondements d’une stratégie d e plantation pour la Grande Muraille Verte. Cah. L’Observatoire Hommes-Milieux Int. Téssékéré 2015, 5, 14.
23. Gillet, H., Note écologique et ethnobotanique sur Calotropis procera. Journal d’agriculture traditionnelle et de botanique appliquée 1968, 15 (12), 543-545. https://doi.org/10.3406/jatba.1968.3007
24. Seignobos, C., L’arbuste Calotropis procera, un épisode de son histoire dans le bassin du lac Tchad. Revue d’ethnoécologie 2016, (9). https://doi.org/10.4000/ethnoecologie.2462
25. Hassan, L. M.; Galal, T. M.; Farahat, E. A.; El-Midany, M. M., The biology of Calotropis procera (Aiton) W.T. Trees 2015, 29 (2), 311-320. https://doi.org/10.1007/s00468-015-1158-7
26. Tripathi, M.; Shukla, P.; Sikarwar, R. L.; Tiwari, A.; Dwivedi, N.; Tripathi, S., Pharmacognostic study of Calotropis procera (Aiton) W.T. Aiton root and stem. Indian Journal of Natural Products and Resources 2022, 374-382.
27. Bannari, A.; Huete, A.; Morin, D.; Zagolski, F., Effets de la couleur et de la brillance du sol sur les indices de végétation. International Journal of Remote Sensing 1996, 17 (10), 1885-1906. https://doi.org/10.1080/01431169608948745
28. Dakouo, D. Effets des types de sol et de la topographie sur l’absorption des ions K+ par le cotonnier (Gossypium h ir su tum L.) dans les agro-systèmes soudano-sahéliens de l’Ouest du Burkina Faso. Université Félix Houphouët-Boigny de Cocody, 2012.
29. Ghannem, N.; Tlili, F.; Riahi, C.; Regaya, K., Étude sédimentologique des dépôts carbonatés continentaux de type palustre de la région de Tajerouine, Nord-Ouest de la Tunisie. Carnets Geol. 2016, 16 (04), 43. https://doi.org/10.4267/2042/58976
30. Karolkowski, A.; Belloir, C.; Briand, L.; Salles, C., Non-Volatile Compounds Involved in Bitterness and Astringency of Pulses: A Review. Molecules (Basel, Switzerland) 2023, 28 (8), 3298. https://doi.org/10.3390/molecules28083298 PMid:37110532 PMCid:PMC10141849
31. Drewnowski, A.; Gomez-Carneros, C., Bitter taste, phytonutrients, and the consumer: a review. The American journal of clinical nutrition 2000, 72 (6), 1424-1435. https://doi.org/10.1093/ajcn/72.6.1424 PMid:11101467
32. Enneb, H.; Belkadhi, A.; Cheour, F.; Ferchichi, A., Comparaison des composés phénoliques et du pouvoir antioxydant de la plante de henné (Lawsonia inermis L.). Journal of New Sciences 2015, 20.
33. Chabrier, J. Y. Plantes médicinales et formes d’utilisation en Phytothérapie. . Université Henri Poincare - Nancy 1, 2010.
34. Lehmann, H. Le médicament à base de plantes en Europe: statut, enregistrement, contrôles. Université de Strasbourg, 2013.
35. Barrow, N. J.; Hartemink, A. E., The effects of pH on nutrient availability depend on both soils and plants. Plant and Soil 2023, 487 (1), 21-37. https://doi.org/10.1007/s11104-023-05960-5
36. Ouedraogo, S.; Traoré, S.; Sombié, B.; Traoré, T.; Nitiéma, M.; BeLeMNaBa, L.; OUEDRAOGO, N.; TIENDREBEOGO, A.; LOMPO, M.; SEMDE, R., Contrôle qualité et étude de comprimabilité des poudres de feuille de Moringa oleifera (lam) et de pulpe du fruit de Adansonia digitata. Sciences de la Santé 2018, 41 (2), 93-101.
37. Spiroux, C., Optimisation des excipients et ingrédients pour la mise en gélules de compléments alimentaires avec une approche Clean label. 2024.
38. Nirala, S., The Phytochemical and antioxidant activity of calotropis procera. CSVTU International Journal of Biotechnology Bioinformatics and Biomedical 2021, 5, 67-73. https://doi.org/10.30732/IJBBB.20210503004
39. Kinda, P. T.; Nacoulma, A. P.; Guenné, S.; Compaoré, M.; Djandé, A.; Lagnika, L.; Kiendrébéogo, M., The Metabolomic study of Calotropis procera Ait. from Burkina Faso, based on chemical functional groups profiling using FTIR. Journal of complementary & integrative medicine 2020, 17 (3). https://doi.org/10.1515/jcim-2019-0134 PMid:32543456
40. Aïssi, A.; Kougblenou, D. S.; Dougnon, V.; Klotoé, J.; Bankolé, H.; Deguenon, Y.; Degbey, C.; Montcho, S.; Fanou, B.; Fah, L.; Edorh, P. A.; Loko, F., Evaluation de la qualité sanitaire des poudres de feuilles de Moringa oleifera Lam. commercialisées au profit des Personnes Vivant avec le VIH à Cotonou (Bénin). International Journal of Biological and Chemical Sciences 2014, 7, 1461. https://doi.org/10.4314/ijbcs.v7i4.4
41. Darkwah, S.; Agbettor, D.; Codjoe, F.; Donkor, E. S., Microbial Contamination of Herbal Preparations on the Ghanaian Market, Accra. Microbiology insights 2022, 15, 11786361221139602. https://doi.org/10.1177/11786361221139602 PMid:36467108 PMCid:PMC9716451
42. Páll, E.; Niculae, M.; Kiss, T.; Şandru, C. D.; Spînu, M., Human impact on the microbiological water quality of the rivers. Journal of medical microbiology 2013, 62 (Pt 11), 1635-1640. https://doi.org/10.1099/jmm.0.055749-0 PMid:23813274 PMCid:PMC3809112
43. Cahagnier, B.; Jacobsen, E.; Lessard, F., Des moisissures aux mycotoxines : signification écophysiologique de marqueurs biochimiques de croissance fongique et prévision des durées de conservation sans risque de détérioration. 2001.
44. Ahiabor, W. K.; Darkwah, S.; Donkor, E. S., Microbial Contamination of Herbal Medicines in Africa, 2000-2024: A Systematic Review. Environmental Health Insights 2024, 18, 11786302241293345. https://doi.org/10.1177/11786302241293345 PMid:39494046 PMCid:PMC11528601
45. Karkey, A.; Jombart, T.; Walker, A. W.; Thompson, C. N.; Torres, A.; Dongol, S.; Tran Vu Thieu, N.; Pham Thanh, D.; Tran Thi Ngoc, D.; Voong Vinh, P.; Singer, A. C.; Parkhill, J.; Thwaites, G.; Basnyat, B.; Ferguson, N.; Baker, S., The Ecological Dynamics of Fecal Contamination and Salmonella typhi and Salmonella paratyphi A in Municipal Kathmandu Drinking Water. PLoS neglected tropical diseases 2016, 10 (1), e0004346. https://doi.org/10.1371/journal.pntd.0004346 PMid:26735696 PMCid:PMC4703202
46. Foster, T. J., The Staphylococcus aureus “superbug”. The Journal of clinical investigation 2004, 114 (12), 1693-1696. https://doi.org/10.1172/JCI200423825 PMid:15599392 PMCid:PMC535074
47. Ondusko, D. S.; Nolt, D., Staphylococcus aureus. Pediatrics in review 2018, 39 (6), 287-298. https://doi.org/10.1542/pir.2017-0224 PMid:29858291
48. Schottroff, F.; Lasarus, T.; Stupak, M.; Hajslova, J.; Fauster, T.; Jäger, H., Decontamination of herbs and spices by gamma irradiation and low-energy electron beam treatments and influence on product characteristics upon storage. Journal of Radiation Research and Applied Sciences 2021, 14 (1), 380-395. https://doi.org/10.1080/16878507.2021.1981112
49. Mrozek-Szetela, A.; Rejda, P.; Wińska, K., A Review of Hygienization Methods of Herbal Raw Materials. Applied Sciences 2020, 10 (22), 8268. https://doi.org/10.3390/app10228268
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Copyright (c) 2026 Salfo Ouedraogo , Rokia Sanogo , Noufou Ouédraogo , Lazare Belemnaba , Moumouni Koala , Mathieu NITIEMA , Rene Dofini Magnini , Boukari Kabore, Axelle Astrid Lofo , Souleymane Compaore , Manegrebem Adele Ouedraogo , Tata Kadiatou Traore , Sylvin Ouédraogo
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