Pharmacognostic, Physicochemical, Phytochemical Characterization and β-Sitosterol-Based HPTLC Fingerprinting of the Methanolic Leaf Extract of Mimusops elengi L.
Abstract
Introduction: Mimusops elengi L. is one of the important plants that have been employed traditionally as a source of herbal medicines for treating inflammation, infections, wounds, and gastrointestinal disorders. Scientific standardization is crucial for the proper identification of herbal drugs.
Objective: The objective of the study was to determine the Pharmacognostic standards, physicochemical properties and quantitative estimation of major phytoconstituents along with chromatographic fingerprinting using β-sitosterol of Mimusops elengi L. leaves.
Materials and Methods: Fresh leaves were collected, authenticated, shade dried, powdered and macerated in methanol. Pharmacognostic studies, Physicochemical parameters such as loss on drying, ash value, extraction value, and pH were determined by standard methods. Phytochemical analysis was done qualitatively and quantitatively by spectrophotometry. HPTLC profiling was done with toluene: ethyl acetate (6:4, v/v) as mobile phase and β-sitosterol as reference standard at 254 and 366 nm.
Results: Macroscopic and microscopic characters helped in the identification of the leaves. The physicochemical analysis was carried out, and results were satisfactory for the quality with loss on drying (7.12 ± 0.18 %), total ash (8.34 ± 0.15 %), acid-insoluble ash (1.21 ± 0.08 %), water soluble extractive (18.45 ± 0.39 %), alcohol soluble extractive (22.78 ± 0.42 %), and pH (6.42 ± 0.03). The extract had high concentrations of phenolics (169.18 ± 0.98 mg GAE/g), flavonoids (58.43 ± 1.87 mg QE/g), tannins (50.31 ± 1.54 mg TAE/g), saponins (42.53 ± 1.26 mg DE/g), and alkaloids (34.76 ± 1.08 mg AE/g). HPTLC fingerprinting yielded unique bands, confirming
Conclusion: The findings establish reliable quality control and future phytopharmaceutical development.
Keywords: Mimusops elengi; pharmacognostic evaluation; physicochemical analysis; HPTLC fingerprinting; β-sitosterol; phenolic content; herbal standardization.
Keywords:
Mimusops elengi, Pharmacognostic evaluation, Physicochemical analysis, HPTLC fingerprinting, β-Sitosterol, Phenolic content, Herbal standardizationDOI
https://doi.org/10.22270/jddt.v16i7.7887References
1. Baliga MS, Pai RJ, Bhat HP, Palatty PL, Boloor R. Chemistry and medicinal properties of the Bakul (Mimusops elengi L.): A review. Food Res Int. 2011;44(7):1823-1829. doi:10.1016/j.foodres.2011.01.063. https://doi.org/10.1016/j.foodres.2011.01.063
2. Gami B, Parabia MH. Ethnobotanical, phytochemical and pharmacological review of Mimusops elengi Linn. Asian Pac J Trop Biomed. 2012;2(9):743-748. doi:10.1016/S2221-1691(12)60220-4. https://doi.org/10.1016/S2221-1691(12)60221-4 PMid:23570006 PMCid:PMC3609369
3. Gillani S, Shahwar D. Investigation of antioxidant activity in Mimusops elengi. J Plant Biochem Physiol. 2017;5(4):202. doi:10.4172/2329-9029.1000202. https://doi.org/10.4172/2329-9029.1000202
4. Sampathkumar Y, Mahadevan SG, Jayaraman R. Physicochemical, phytochemical screening and HPTLC fingerprinting analysis of ethanolic extract of Mimusops elengi Linn. leaves. Res J Pharm Technol. 2020;13(5):2091-2095. doi:10.5958/0974-360X.2020.00376.5. https://doi.org/10.5958/0974-360X.2020.00376.5
5. Gharpankar PD, Kadam PV, Kamble NS. Review on Mimusops elengi Linn: Phytochemical properties and pharmacological action as medicinal plant and its importance. Res J Pharmacol Pharmacodyn. 2024;16(1):1-5. doi:10.52711/2321-5836.2024.00001. https://doi.org/10.52711/2321-5836.2024.00001
6. Evans WC. Trease and Evans’ Pharmacognosy. 16th ed. Edinburgh: Elsevier; 2009.
7. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman & Hall; 1998.
8. Khandelwal KR. Practical Pharmacognosy: Techniques and Experiments. 19th ed. Pune: Nirali Prakashan; 2008.
9. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. New York: Springer; 2007. https://doi.org/10.1007/978-0-387-70638-2 PMCid:PMC2705749
10. World Health Organization. Quality Control Methods for Herbal Materials. Geneva: World Health Organization; 2011.
11. World Health Organization. WHO Guidelines on Good Agricultural and Collection Practices (GACP) for Medicinal Plants. Geneva: World Health Organization; 2003.
12. Government of India, Ministry of AYUSH. The Ayurvedic Pharmacopoeia of India. Part I, Vol. VI. New Delhi: Government of India; 2008.
13. Shinde M, Bais SK, Devmare D. Review on standardization of herbal drug and formulation. Int J Pharmacogn Life Sci. 2023;4(1):20-24. doi:10.33545/27072827.2023.v4.i1a.69. https://doi.org/10.33545/27072827.2023.v4.i1a.69
14. Wang H, et al. Advancing herbal medicine: Enhancing product quality and safety through robust quality control. Chin Med. 2023;18:98. doi:10.1186/s13020-023-00839-z. https://doi.org/10.3389/fphar.2023.1265178 PMid:37818188 PMCid:PMC10561302
15. Maheshwaran L, Nadarajah L, Senadeera SPNN, Ranaweera CB, Chandana AK, Pathirana RN. Phytochemical testing methodologies and principles for preliminary screening/qualitative testing. Asian Plant Res J. 2024;12(5):11-38. doi:10.9734/APRJ/2024/v12i5267.
https://doi.org/10.9734/aprj/2024/v12i5267
16. Maheshwaran L, Nadarajah L, Senadeera SPNN, Ranaweera CB, Chandana AK, Pathirana RN. Phytochemical testing methodologies and principles for preliminary screening/qualitative testing. Asian Plant Res J. 2024;12(5):11-38. doi:10.9734/APRJ/2024/v12i5267.
https://doi.org/10.9734/aprj/2024/v12i5267
17. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-178. doi:10.1016/S0076-6879(99)99017-1. https://doi.org/10.1016/S0076-6879(99)99017-1
18. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10(3):178-182. doi:10.38212/2224-6614.2748. https://doi.org/10.38212/2224-6614.2748
19. Limpabandhu T, Khammanit R, Thitithanakul S, Chansakaow S. Pharmacognostic standardization and phytochemical evaluation of Ficus rumphii Blume leaves in Thailand. Phytomed Plus. 2024;4(4):100661. doi:10.1016/j.phyplu.2024.100661. https://doi.org/10.1016/j.phyplu.2024.100661
20. Jain V, Sahu U, Chauhan NS, Parihar AKS, Karbhal KS, Inchulkar SR. HPTLC method development and quantification of marker compound eugenol in Ayurvedic polyherbal formulations: Avipattikar churna. Tradit Med Mod Med. 2023;6(1):2350007. doi:10.1142/S2575900023500076. https://doi.org/10.1142/S2575900023500076
21. Kaur N, Kumar V, Singh R. HPTLC-based chemical fingerprinting and quantification of kaempferol in Bauhinia purpurea extracts for quality control assessment. J Planar Chromatogr Mod TLC. 2024;37(2):145-153. doi:10.1007/s00764-024-00288-w.
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