Formulation and Characterization of Quercetin–Silver Nitrate Complex Using FTIR Analysis
Abstract
Quercetin, a flavonoid with potent antioxidant and therapeutic properties, faces limitations due to poor aqueous solubility and bioavailability. This study reports the formulation of a quercetin–silver nitrate complex via a simple solution complexation method using ethanol as solvent in a 1:1 molar ratio. Quercetin was dissolved in absolute ethanol, followed by dropwise addition of silver nitrate solution under stirring at 30–40°C for 3–4 hours, with light protection to prevent photodegradation. The mixture was aged for 12–24 hours, evaporated to dryness, and purified in a desiccator. FTIR characterization confirmed complex formation through shifts in key bands: phenolic O–H stretching (3200–3500 cm⁻¹) broadened due to hydrogen bonding and Ag⁺ coordination; C=O stretching (~1650 cm⁻¹) shifted, indicating carbonyl oxygen involvement; C–O stretches (1200–1300 cm⁻¹) altered, suggesting phenolic group participation; and new Ag–O vibrations (500–700 cm⁻¹) evidenced metal–ligand bonding, primarily via 3-OH/4-C=O chelation or catechol moiety.
Keywords: Quercetin, Silver Nitrate Complex, FTIR Analysis, Colour Change, Complex Formation
Keywords:
Quercetin, Silver Nitrate Complex, FTIR Analysis, Colour Change, Complex FormationDOI
https://doi.org/10.22270/jddt.v16i7.7879References
1. Ahmad S, Munir S, Zeb N, Ullah A, Khan B, Ali J, et al. Green nanotechnology: A review on green synthesis of silver nanoparticles-An ecofriendly approach. Taylor & Francis. 2019;14:5087-107. PubMed PMID: 31371949. https://doi.org/10.2147/IJN.S200254 PMid:31371949 PMCid:PMC6636611
2. Lafta MZ, Hameed Al-Samarrai RR, Bouaziz M. Green synthesis of silver and gold nanoparticles using quercetin extracted from Arctium lappa by HPLC, Characterization and Estimation of antioxidant activity. Results Chem. 2025 Jan 1;13:102028. https://doi.org/10.1016/j.rechem.2025.102028
3. Bentz AB. A Review of Quercetin: Chemistry, Antioxident Properties, and Bioavailability. J Young Investig [Internet]. 2017 Apr 1 [cited 2026 Mar 16]. Available from: https://www.jyi.org/2009-april/2017/10/15/a-review-of-quercetin-chemistry-antioxidant-properties-and-bioavailability
4. Singh Baghel S, Shrivastava N, Singh Baghel R, Agrawal P, Rajput S. A REVIEW OF QUERCETIN: ANTIOXIDANT AND ANTICANCER PROPERTIES [Internet]. [cited 2026 Mar 16]. Available from: www.wjpps.com
5. Alizadeh SR, Ebrahimzadeh MA. Quercetin derivatives: Drug design, development, and biological activities, a review. Eur J Med Chem. 2022 Feb 5;229. PubMed PMID: 34971873. https://doi.org/10.1016/j.ejmech.2021.114068 PMid:34971873
6. Sodhi S, Bandichhor R, Kapoor N. Understanding silver nitrate: Characteristics, applications, hazard evaluation, and health implications. Hazardous Chemicals: Overview, Toxicological Profile, Challenges, and Future Perspectives. 2025 Jan 1;607-15. https://doi.org/10.1016/B978-0-323-95235-4.00041-4
7. Ram Kumar Pandian S, Kunjiappan S, Ravishankar V, Sundarapandian V. Synthesis of quercetin-functionalized silver nanoparticles by rapid one-pot approach. Computational Biology and Bionanotechnology. 2021;102(1):75-84. https://doi.org/10.5114/bta.2021.103764 PMid:36605709 PMCid:PMC9642916
8. Sharma R, Basist P, Alhalmi A, Khan R, Noman OM, Alahdab A. Synthesis of Quercetin-Loaded Silver Nanoparticles and Assessing Their Anti-Bacterial Potential. Micromachines 2023, Vol 14, Page 2154. 2023 Nov 25;14(12):2154. https://doi.org/10.3390/mi14122154 PMid:38138323 PMCid:PMC10745049
9. Chahardoli A, Hajmomeni P, Ghowsi M, Qalekhani F, Shokoohinia Y, Fattahi A. Optimization of Quercetin‐Assisted Silver Nanoparticles Synthesis and Evaluation of Their Hemocompatibility, Antioxidant, Anti‐Inflammatory, and Antibacterial effects. Global Challenges. 2021 Dec 1;5(12):2100075. PMID: 34938575. https://doi.org/10.1002/gch2.202100075 PMid:34938575 PMCid:PMC8671616
10. Sharma R, Basist P, Alhalmi A, Khan R, Noman OM, Alahdab A. Synthesis of Quercetin-Loaded Silver Nanoparticles and Assessing Their Anti-Bacterial Potential. Micromachines (Basel). 2023 Dec 1;14(12). PMID: 38138323. https://doi.org/10.3390/mi14122154 PMid:38138323 PMCid:PMC10745049
11. Krutyakov 0.811Q1SJR Q1; 0.811NAABS NANAABDC NARussian Chemical ReviewsYA, Kudrinskiy A, … AORC, 2008 undefined. Synthesis and properties of silver nanoparticles: advances and prospects. iopscience.iop.org. 2008 Mar 31;77(3):233-57. https://doi.org/10.1070/RC2008v077n03ABEH003751
12. Asif M, Yasmin R, Asif R, Ambreen A, Mustafa M, Umbreen S. Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential. Dose-Response. 2022 Apr 11;20(2):15593258221088708. PMID: 35592270. https://doi.org/10.1177/15593258221088709 PMid:35592270 PMCid:PMC9112420
13. Alugoju P, Narsimulu D, Bhanu JU, Satyanarayana N, Periyasamy L. Role of quercetin and caloric restriction on the biomolecular composition of aged rat cerebral cortex: An FTIR study. Spectrochim Acta A Mol Biomol Spectrosc. 2019 Sep 5;220:117128. doi:10.1016/j.saa.2019.05.
14. Kokalj Ladan M, Straus J, Tavčar Benković E, Kreft S. FT-IR-based method for rutin, quercetin and quercitrin quantification in different buckwheat (Fagopyrum) species. Scientific Reports 2017 7:1. 2017 Aug 3;7(1):7226-. PubMed PMID: 28775318. https://doi.org/10.1038/s41598-017-07665-z PMid:28775318 PMCid:PMC5543106
15. Aiswarriya GR, Gayathri R, Priya Veeraraghavan V, Sankaran K, Prakash Francis A. Green Synthesis, Characterization and Biocompatibility Study of Quercetin-Functionalized Biogenic Silver Nanoparticles. https://doi.org/101142/S1793292023500558. 2023 Jul 5;18(7).
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Copyright (c) 2026 Vinay Kumar Yadav , Vikas Kumar , Amita Verma
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