Toxicological assessment of the hydroethanolic extract of Sterculia setigera leaves', a neuroactive medicinal plant of Togolese flora
Abstract
Background: Sterculia setigera is a plant used in traditional medicine for many purposes. We previously reported that its dry hydroethanolic leaf extract (SSE) protected cerebellar granule neurons and PC12 cells against H₂O₂, 6-OHDA, and serum deprivation-induced cell death, and mitigated ethanol neurotoxicity in the cerebellar cortex of neonatal Wistar rats while preserving motor coordination. This study aims to establish the toxicological profile of Sterculia setigera leaves' hydroethanolic extract, using appropriate in vitro and in vivo models.
Methods : The brine shrimp (Artemia salina) lethality assay served as a cytotoxicity test (in vitro model). An acute oral toxicity test was conducted according to OECD TG 423 (2000 mg/kg). The repeated dose oral toxicity of 28 days was performed following the OECD TG 407 (100, 200, and 400 mg/kg) on Wistar rats.
Results: The 50% lethal concentration (LC50) was 1.84 mg/mL. Based on acute oral toxicity, the LD50 value of the extract was up to 2000 mg/kg of body weight. The 28-day repeated oral administration of Sterculia setigera leaves hydroethanolic extract did not provoke negative effects on rats, as they were safe based on body and organ weight, hematological, biochemical, and histopathological results.
Conclusion : These results indicate that the dry hydroethanolic extract of S. setigera leaves did not provoke adverse effects that can lead to death or internal histological lesions in Wistar rats after 28-day oral administration.
Keywords: Sterculia setigera; cytotoxicity; Artemia salina; toxicological profile
Keywords:
Sterculia setigera , cytotoxicity , toxicological profileDOI
https://doi.org/10.22270/jddt.v16i7.7866References
1. Ahlidja W, Amegayibor EF, Addae L, Abor EK, Korsah HM, Opoku-Kwabi D, Armah FA, and Henneh IT. "Pharmacological and phytochemical properties of a promising African medicinal plant, Sterculia setigera Delile: A systematic review". Sci Afr. 2024; 24: 02142. DOI: http://dx.doi.org/10.1016/j.sciaf.2024.e02142
2. Atakpama W, Batawila K, Gnamkoulaba A, and Akpagana K. "Quantitative Approach of Sterculia setigera Del. (Sterculiaceae) Ethnobotanical Uses Among Rural Communities in Togo (West Africa)". Ethnobot Res Appl. 2015; 14: 063‑080.
3. Manisha M, Babu R, Begam AM, Shakya Chahal K, and Ashok Harale A. "Medicinal Plants and Traditional Uses and Modern Applications". J Neonatal Surg. 2025; 14 (3): 162‑175. DOI: http://dx.doi.org/10.52783/jns.v14.2210
4. Saggar S, Mir PA, Kumar N, Chawla A, Uppal J, Shilpa S, Kaur A. "Traditional and Herbal Medicines: Opportunities and Challenges". Pharmacogn Res. 2022; 14(2): 107-114. DOI: https://doi.org/10.5530/PRES.14.2.15
5. Latif R, and Nawaz T. "Medicinal plants and human health: a comprehensive review of bioactive compounds, therapeutic effects, and applications". Phytochem Rev. 2026; 25(3): 2299‑342. DOI: http://dx.doi.org/10.1007/s11101-025-10194-7
6. Majolo F, de Oliveira Becker Delwing LK, Marmitt DJ, Bustamante-Filho IC, Goettert M.I. "Medicinal plants and bioactive natural compounds for cancer treatment: Important advances for drug discovery". Phytochem Lett. 2019; 31: 196‑207. DOI: http://dx.doi.org/10.1016/j.phytol.2019.04.003
7. World Health Organization; 2013. "WHO traditional medicine strategy: 2014-2023". Available at: https://www.who.int/publications/i/item/9789241506096 . Accessed May 11, 2026.
8. Dossou-Yovo KM, Diallo A, Lawson-Evi P, Kantati YT, Darré T, Bakoma B, Eklu-Gadegbeku K. "A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats". J Toxicol. 2021; 1: 5570206. DOI: http://dx.doi.org/10.1155/2021/5570206
9. Kantati YT, Kodjo MK, Lefranc B, Basille-Dugay M, Hupin S, Schmitz I, Leprince J, Gbeassor M, Vaudry D. "Neuroprotective Effect of Sterculia setigera Leaves Hydroethanolic Extract". J Mol Neurosci. 2024; 74(2): 44. DOI: http://dx.doi.org/10.1007/s12031-024-02222-6
10. Kantati YT, Kodjo MK, Kombate B, Togbossi LA, Eklu-Gadegbeku K, and Gbeassor M. "Sterculia setigera hydroethanolic extract protects brain tissues ex vivo against lipid peroxidation and possesses in vitro antioxidant and anti-inflammatory properties". J Drug Deliv Ther. 2022; 12(5-S): 118-122. DOI: http://dx.doi.org/10.22270/jddt.v12i5-s.5643
11. Aliyu I, Katsayal U, Abdulrahman E, Olorukooba A, Jenson B, Asma’i SK, Umar A. "Phytochemical and In vivo Anti-Plasmodial Evaluation of N-Butanol and Aqueous Fractions of the Leaf of Sterculia setigera Del. (Sterculiaceae)". Pharm Dev Ind Pharm. 2020; 2(2): 20-32.
12. Farzaei MH, Bayrami Z, Farzaei F, Aneva I, Das SK, Patra JK, Das G, Abdollahi M. "Poisoning by Medical Plants". Arch Iran Med. 2020; 23(2): 117-127. PMID: 32061075.
13. OECD/OCED; 2025. "Test No. 407: Repeated Dose 28-day Oral Toxicity Study in Rodents", in OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris, 2025, pp. 1-15. DOI: https://doi.org/10.1787/9789264070684-en
14. Dossou-Yovo KM, Diallo A, Lawson-Evi P, Darré T, Bakoma B, Eklu-Gadégbéku K. "Cytotoxicity, Acute, and Subacute Study of Hydroalcoholic Root Extract of Carissa spinarum L. on Wistar Rats". Med Food. 2021; 24(7): 756‑761. DOI: https://doi.org/10.1089/jmf.2020.0065
15. OCDE/OCED; 2002. "Test No. 423: Acute Oral toxicity - Acute Toxic Class Method", in OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris, 2002. DOI : https://doi.org/10.1787/9789264071001-en
16. Sarah QS, Anny FC, Misbahuddin M. "Brine shrimp lethality assay". Bangladesh J Pharmacol. 2017; 12(2): 186-189. DOI: https://doi.org/10.3329/bjp.v12i2.32796
17. Mousseux M. "Test de toxicité sur larves d'Artemia salina: entretien d'un élevage de balanes". In Rapport de stage de 2ème année, DEUST Aquaculture, Univ. Française du Pacifique, Nouméa (Centre IRD, fdi:43863), France, 1995. https://www.documentation.ird.fr/hor/fdi:43863
18. Taha NMM, Mudawi BM. "Antioxidant and Cytotoxic Activities of Sterculia setigera Del.". Am J Res Commun. 2018; 6(5): 41-49.
19. Muhammad A, Idris M, and Mamman AH. "In vitro Screening of Potential Antioxidative and Toxicity of the Stembark Extracts from Sterculia setigera Del". Algerian J. Nat. Products. 2020; 8: 774-779. DOI: https://doi.org/10.5281/ZENODO.3973337
20. Silva AV, Norinde U, Liiv E, Platzack B, Oberg M, Tornqvist E. "Associations Between Clinical Signs and Pathological Findings in Toxicity Testing". ALTEX-Altern Anim Ex. 2021; 38(2): 198-214. DOI: https://doi.org/10.14573/altex.2003311
21. Ramaiah SK. "A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters". Food Chem Toxicol. 2007; 45(9): 1551‑1557. DOI: https://doi.org/10.1016/j.fct.2007.06.007
22. Clark VL, Kruse JA. "Clinical Methods: The History, Physical, and Laboratory Examinations". JAMA. 1990; 264(21): 2808‑2809. DOI: https://doi.org/10.1001/jama.1990.03450210108045
23. Weinblatt ME, Fraser P. "Elevated mean corpuscular volume as a predictor of hematologic toxicity due to methotrexate therapy". Arthritis Rheumatol. 1989; 32(12): 1592-1596. DOI: https://doi.org/10.1002/anr.1780321214
24. Zaruwa MZ, Ibok NI, Ibok IU, Onyenonachi EC, Danchal C, Ahmed AG, Ahmed MU, Sudi IY. "Effects of Sterculia setigera Del. Stem Bark Extract on Hematological and Biochemical Parameters of Wistar Rats". Biochem Insights. 2016; 9: 19-22. DOI: https://doi.org/10.4137/BCI.S36143
25. Farhana A, Lappin SL. "Biochemistry, Lactate Dehydrogenase. [Updated 2023 May 1]". In StatPearls Treasure Island (FL), StatPearls Publishing LLC, 2026. https://www.ncbi.nlm.nih.gov/books/NBK557536/
26. Kantati YT, Kodjo MK, Lefranc B, Eklu-Gadegbeku K, Leprince J, Vaudry D. "Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death". IBRO Neurosci Rep. 2025; 19 : 110-116. DOI: https://doi.org/10.1016/j.ibneur.2025.06.007
27. Kunutsor SK, Bakker SJ, Gansevoort RT, Chowdhury R, Dullaart RP. "Circulating total bilirubin and risk of incident cardiovascular disease in the general population". Arterioscler Thromb Vasc Biol. 2015; 35(3): 716-724. DOI: https://doi.org/10.1161/ATVBAHA.114.304929
28. Corns CM. "Herbal remedies and clinical biochemistry". Annals of Clinical Biochemistry. 2003; 40(5): 489-507. DOI: https://doi.org/10.1258/000456303322326407
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Copyright (c) 2026 Yendubé T. Kantati , Akomagni D.C. Donko , Komlan M. Dossou-Yovo , Essotolom Badjabaissi , Sabrina C.J. Sanvee , Aboudoulatif Diallo , Kossi Metowogo , Povi Lawson-Evi , Amegnona Agbonon , Magloire K. Kodjo , Aklesso Mouzou , Kwashie Eklu-Gadegbeku
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