Potentiation of antimalarial activity in Artemisia annua cultivated in the presence of fodder peanuts and beneficial microorganisms
Abstract
Production of Artemisia annua chemical active ingredients remains too expensive and unprofitable. Its antimalarial active molecules vary a lot with environmental diversity and are low concentrated in the plant. Research into ways of optimizing their content, extraction and concentration is a current challenge. In this study, we tried to promote an inexpensive and more profitable cultivation procedure to improve Artemisia antimalarial biomass and therapeutic molecules. Seeds of A. annua were grown on a non-sterilized soil substrate using non-randomized bloc design. Impact of Arachis pintoï (Ara), special organic matter (MOS), Biochar (Bio), mycorrhizal fungi (CMA) and endophyte bacteria (End) on the parameters of biomass yield in A. annua was evaluated as well as contents of some phytochemical active biomolecules, antioxidant capacity and in vitro PfLDH-based antiplasmodial activity of A. annua. Increased biomass yield in A. annua was higher with Arachis pintoï (Ara) combined to special organic matter. Artemisia annua in “Ara+CMA+End” system was more efficient compare to Artemisia annua without biofertilizer. We had respectively, 86.88±3.38 and 65.77±3.08 mgEqAG/ g of DM for plyphonols; 79.18±5.84 and 67.82±4.38 mgEqAA/ g of DM for antioxidant capacity; regarding antiplasmodial activity we had for 50% Inhibitory Concentration (IC50) in µg/mL: 31±2.05 and 60.68± 13.07 for PfDd2 (multidrug-resistant); 34.14 ± 2.71 and 47.69 ± 6.34 for Pf3D7 (sensitive). Integrated soil fertility management (ISFM) involving a forage legume and microbial fertilizers may be the best option to provide the highest bioactive molecules content and diversity. ISFM improve Artemisia annua biomass production for better active therapeutic ingredients.
Keywords: Microbial biofertilizers, Arachis pintoï, malaria active biomolecules, Artemisia annua
Keywords:
Microbial biofertilizers, Arachis pintoï, malaria active biomolecules, Artemisia annuaDOI
https://doi.org/10.22270/jddt.v16i4.7698References
1. Antony Ellman. Cultivation of Artemisia annua in Africa and Asia. Outlooks on Pest Management. 2012; 1(2):84-88. https://doi.org/10.1564/21apr08
2. Maximilian Johannes Huter, Axel Schmidt, Fabian Mestmäcker, Maximilian Sixt and Jochen Strube. Systematic and Model-Assisted Process Design for the Extraction and Purification of Artemisinin from Artemisia annua L.-Part IV: Crystallization. Processes 2018; 6: 181. https://doi.org/10.3390/pr6100181
3. Khalid, M., Hassani, D., Bilal, M., Asad, F et Huang, D. Influence du bio-engrais contenant des champignons bénéfiques et des bactéries rhizosphériques sur les composés favorisant la santé et l'activité antioxydante de Spinacia oleracea L. Botanical. Étalon 2017 ; 58 : 35. https://doi.org/10.1186/s40529-017-0189-3 PMid:28815474 PMCid:PMC5559411
4. Ha Dinh, T., Husson, O., Chabanne, O., Lienhard, P and Séguy, L. (2002). Arachis pintoï a living vegetal covering orchards. Poster. In : Des approches innovantes au service du développement sur l'agriculture, les services aux producteurs et les politiques agricoles ? Colloque International des approches innovantes au service du développement agricole. Séminaire national, Hanoï, Vietnam. Maison des Éditions de l'Agriculture, 11:50 - 58.
5. Kamseu, J. P., Kuete, F. M., Njemen, N. D. E., Chime, N. L. L., Ga'amgne, O. F. E., Lakeu, M. I., Njionji, T. A et Kamdem, T. A. G. (2021). Effet de la co-inoculation des champignons mycorhiziens et Rhizobium sur les performances agronomiques de six variétés de soja (Glycine max L.) à l'Ouest Cameroun : Cas de l'arrondissement de Dschang. International Journal of Progressive Sciences and Technology. 14 : 97-110.
6. Nwaga, D., Jansa, J., Abossolo, A. M and Frossard, E. (2010). The potential of soil beneficial microorganisms for slash-and-burn agriculture in the humid forest zone of subsaharan AfricaIn: soil biology and agriculture in the tropics. Springer Heidelberg Dordrecht. London, New York, 80-107. https://doi.org/10.1007/978-3-642-05076-3_5
7. Obe, B. M. T. (2023). Utilisation de microorganismes bénéfiques et de la matière organique pour stimuler la croissance et la synthèse de molécules fonctionnelles chez l'armoise africaine (Artemisia afra), Mémoire de Master, Université de Yaoundé I/Cameroun, 85.
8. Bulda, O. V., Rassadina, V. V., Alekseichuk, H. N et Laman, N. A. (2008). Spectrophotométrie dosage des carotènes, xanthophylles et chlorophylles dans les extraits de graines de plantes. Russe. Journal Physiologie des plantes. 55(4) : 544-551.
9. F. Kouoh Elombo, C. E. Yando, R. V. Djuikwoa, H. Abdul, R. S. Touole, E. Fokou and P. Moundipa ; Fewou. GRAPTOPHYLLUM PICTUM RESTORES IN THREE DAYS HAEMATOLOGIC PARAMETERS AFTER PHENYLHYDRAZINE-INDUCED ANEMIA OF INFLAMMATION IN WISTAR RATS. IJPSR 2026; Vol. 17(6): 1000-08.
10. Ridel Mbiandou Njami ²² , Ferdinand Kouoh Elombo, Sylvain Nsangou Pechangou, Bradley Bolling, Frederic Nico Njayou and Paul Fewou Moundipa. A concentrated phenolic compounds extract from Khaya grandifoliola CDC exhibits anti-oxidant and anti-TNFα activities. Journal of Pharmacognosy and Phytochemistry 2024; 13(3): 06-12. https://doi.org/10.22271/phyto.2024.v13.i3a.14936
11. Ellman, G. L. (1959). Tissue Sulfhydryl Groups. Archives of Biochemistry and Biophysics 82, 70-77. https://doi.org/10.1016/0003-9861(59)90090-6 PMid:13650640
12. Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976;193(4254):673-675. https://doi.org/10.1126/science.781840 PMid:781840
13. Tako Djimefo Alex Kevin, Yamssi Cedric, Noumedem Anangmo Christelle Nadia, Ngouyamsa Nsapkain Aboubakar Sidiki, Mounvera Abdel Azizi, Gamago Nkadeu Guy-Armand, Tientcheu Noutong Jemimah Sandra, Mbohou Nchetnkou Christian, Essangui Same Estelle Géraldine, Tankoua-Tchounda Roméo, Vincent Khan Payne, Lehmann Léopold Gustave. Antiplasmodial, Antioxidant, and Cytotoxic Activity of Bridelia micrantha a Cameroonian Medicinal Plant Used for the Treatment of Malaria. Biomed Res Int. 2023 Apr 11;2023:1219432. https://doi.org/10.1155/2023/1219432 PMid:37082191 PMCid:PMC10113053
14. Lambros C., Vanderberg J. P. Synchronization of Plasmodium falciparum erythrocytic stages in culture. The Journal of Parasitology . 1979;65(3):p. 418. https://doi.org/10.2307/3280287 PMid:383936
15. Smilkstein M., Sriwilaijaroen N., Kelly J. X., Wilairat P., Riscoe M. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrobial Agents and Chemotherapy. 2004;48(5):1803-1806. https://doi.org/10.1128/AAC.48.5.1803-1806.2004 PMid:15105138 PMCid:PMC400546
16. Wang, J.; Diao, R.; Wu, Z.; Wan, S.; Yang, S.; Li, X. Transcriptomic and Metabolomic Analyses Reveal the Roles of Flavonoids and Auxin on Peanut Nodulation. Int. J. Mol. Sci. 2023, 24, 10152. https://doi.org/10.3390/ijms241210152 PMid:37373299 PMCid:PMC10299696
17. 14 Wade, A., Bahdjolbe, M., Hawaou, A., Moukala, S.L. and Nwaga, D. Development of Beneficial-Microbial-Based Biofertilizers for Future Generation of Agriculture (Bio-Agriculture) and Their Global Health Impacts in Cameroon. Advances in Microbiology. 2025, 15, 232-252. https://doi.org/10.4236/aim.2025.154017
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Copyright (c) 2026 Ferdinand Kouoh Elombo , JJ Alladoum Kadounia , M Mbiandjeu Tchoumke , J O Kuamou Nzoutap , D Nwaga
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