Physicochemical Characterization and Thin Layer Chromatographic Fingerprinting of Drakshadi Ghrita: A Foundational Study for Standardization
Abstract
Background: Drakshadi Ghrita is a classical Ayurvedic medicated ghee formulation traditionally indicated in eye care and as a rejuvenative. Despite its clinical use, published analytical data remain scarce, hindering quality assurance and regulatory acceptance.
Objective: To establish a comprehensive physicochemical and chromatographic profile of a marketed Drakshadi Ghrita sample and correlate identified biomarkers with clinical ophthalmic applications.
Methods: A 100 mL sample was analysed following Ayurvedic Pharmacopoeia of India protocols for moisture content, saponification value, iodine value, acid value, Reichert-Meissl value, specific gravity, refractive index, rancidity (Kries test), and thin-layer chromatographic fingerprinting.
Results: The sample exhibited moisture 0.16% w/w, saponification value 223.82, iodine value 37.36, acid value 0.78, Reichert-Meissl value 25.93, specific gravity 0.9211 at 25°C, and refractive index 1.4610 at 25°C. Kries test was negative. TLC revealed four major spots at Rf 0.25, 0.28, 0.79, and 0.96 after anisaldehyde-sulphuric acid derivatisation, corresponding to flavonoids, glycyrrhizin, resveratrol, and sterols respectively.
Conclusion: The sample demonstrated physicochemical parameters consistent with pure bovine ghee base and good manufacturing quality. The TLC fingerprint provides a reproducible identity marker, and the identified biomarkers possess significant clinical relevance in managing anterior and posterior segment eye disorders.
Keywords: Drakshadi Ghrita; medicated ghee; physicochemical analysis; Reichert-Meissl value; thin-layer chromatography; ocular biomarkers
Keywords:
Drakshadi Ghrita, medicated ghee, physicochemical analysis, Reichert-Meissl value, thin-layer chromatography, ocular biomarkersDOI
https://doi.org/10.22270/jddt.v16i4.7675References
[1] Srikantha Murthy KR. Sarngadhara Samhita. Varanasi: Chaukhambha Orientalia; 2001. p. 212-5.
[2] Sharma PV. Dravyaguna Vijnana. Vol. 2. Varanasi: Chaukhambha Bharati Academy; 2003. p. 567-70.
[3] Nassiri-Asl M, Hosseinzadeh H. Review of the pharmacological effects of Vitis vinifera and its bioactive compounds. Phytother Res. 2009;23(9):1197-204. https://doi.org/10.1002/ptr.2761 PMid:19140172
[4] Mukherjee PK. Quality Control and Evaluation of Herbal Drugs. Amsterdam: Elsevier; 2019. p. 421-6. https://doi.org/10.1016/B978-0-12-813374-3.00010-7 PMid:31178657 PMCid:PMC6528832
[5] Ayurvedic Pharmacopoeia Committee. The Ayurvedic Pharmacopoeia of India, Part II, Vol. IV. New Delhi: Ministry of AYUSH; 2017. p. 144-71.
[6] Ayurvedic Pharmacopoeia Committee. The Ayurvedic Pharmacopoeia of India, Part I, Vol. VI. New Delhi: Ministry of AYUSH; 2008. p. 287-9.
[7] Gordon MH. The development of oxidative rancidity in foods. In: Steele R, editor. Understanding and Measuring the Shelf-Life of Food. Cambridge: Woodhead Publishing; 2004. p. 156-75.
[8] Firestone D. Physical and Chemical Characteristics of Oils, Fats, and Waxes. 3rd ed. Washington DC: AOCS Press; 2013. p. 98-102.
[9] Aparnathi K, Sharma R. Indian ghee: an overview. Indian Dairyman. 2017;69(5):62-7.
[10] BIS. Indian Standard for Ghee (IS 3508:2018). New Delhi: Bureau of Indian Standards; 2018. p. 8-11.
[11] Gandhi K, Sharma R, Gautam PB, Mann B. Chemical quality of ghee prepared from different milk types. Indian J Dairy Sci. 2019;72(4):382-7.
[12] Kumar A, Tripathi S, Hans N, Tewari R. Adulteration of ghee: a review. Int J Chem Stud. 2020;8(5):2241-5.
[13] Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer; 1996. p. 305-10. https://doi.org/10.1007/978-3-642-00574-9
[14] Kubota S, Ozawa Y, Kurihara T, et al. Roles of AMP-activated protein kinase in diabetes-induced retinal inflammation. Invest Ophthalmol Vis Sci. 2011;52(12):9147-52. https://doi.org/10.1167/iovs.11-8041 PMid:22058332
[15] Lindsey JD, Duong-Polk KX, Hammond D, et al. Protection of injured retinal ganglion cells by resveratrol in a rat model of glaucoma. Invest Ophthalmol Vis Sci. 2015;56(7):4281-90.
[16] Isbrucker RA, Burdock GA. Risk and safety assessment on the consumption of Licorice root. Regul Toxicol Pharmacol. 2006;46(3):167-92. https://doi.org/10.1016/j.yrtph.2006.06.002 PMid:16884839
[17] Higuchi R, Kawakita T, Yoshida Y, et al. The anti-inflammatory effect of glycyrrhizin in a mouse model of dry eye disease. J Ocul Pharmacol Ther. 2012;28(4):388-94.
[18] Canani RB, Costanzo MD, Leone L, et al. Potential beneficial effects of butyrate in intestinal and extraintestinal diseases. World J Gastroenterol. 2011;17(12):1519-28. https://doi.org/10.3748/wjg.v17.i12.1519 PMid:21472114 PMCid:PMC3070119
[19] Leonardi A, Castegnaro A, Valerio AL, Lazzarini D. Epidemiology of allergic conjunctivitis. Curr Opin Allergy Clin Immunol. 2015;15(5):482-8. https://doi.org/10.1097/ACI.0000000000000204 PMid:26258920
[20] Sharma R, Amin H, Galib R, Prajapati PK. Therapeutic potentials of Netra Tarpana: a review. Ayu. 2014;35(2):127-32
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Copyright (c) 2026 Sagarika S Lal , Harish Kumar Singhal , Manisha Goyal , Rajeev Soni
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