Formulation and Antibacterial Evaluation of a Glycerin Soap Incorporated with Cymbopogon citratus Essential Oil
Abstract
oap remains one of the most widely used hygiene products for reducing microbial contamination of the skin. In recent years, interest has increased in the development of natural antimicrobial agents as alternatives to synthetic antiseptics. The present study aimed to formulate a glycerin-based soap incorporating Cymbopogon citratus essential oil and to evaluate its physicochemical properties and antibacterial activity.
Different soap formulations were prepared using coconut oil, sodium hydroxide, glycerin, ethanol, sugar and distilled water. Increasing concentrations of Cymbopogon citratus essential oil (0.5–2.5%) were incorporated to obtain formulations S1–S5, while S0 served as the reference formulation without essential oil. The physicochemical properties of the soaps were evaluated by determining pH and foaming capacity. Antibacterial activity was assessed using an experimental contamination model involving sterile gloves inoculated with Escherichia coli, followed by washing and microbiological analysis.
The results showed that the formulated soaps exhibited pH values ranging from 9.0 to 12.1. Foaming tests demonstrated satisfactory foaming ability, with the highest foam height observed for formulation S2. Antibacterial evaluation revealed that formulations containing higher concentrations of essential oil (S4 and S5) showed complete inhibition of bacterial growth after washing, comparable to commercial antiseptic soaps.
These findings suggest that incorporation of Cymbopogon citratus essential oil into glycerin-based soap may represent a promising approach for developing natural antibacterial cleansing products. Further studies involving additional microorganisms and dermatological tolerance evaluations are recommended.
Keywords : Soap formulation; Glycerin soap; Cymbopogon citratus; Essential oil; Antibacterial activity; Natural antiseptic
Keywords:
Soap formulation, Glycerin soap, Cymbopogon citratus, Essential oil, Antibacterial activity, Natural antisepticDOI
https://doi.org/10.22270/jddt.v16i4.7656References
1. Spitz L. Soap Manufacturing Technology. AOCS Press; 2009.
2. World Health Organization. WHO Guidelines on Hand Hygiene in Health Care. WHO Press; 2009.
3. Fluhr JW, Darlenski R, Surber C. Glycerol and the skin: holistic approach to its origin and functions. Acta Derm Venereol. 2008;88:215-221. https://doi.org/10.1111/j.1365-2133.2008.08643.x PMid:18510666
4. Simmons WH, Appleton HA. The Handbook of Soap Manufacture. London; 1912.
5. Burt S. Essential oils: their antibacterial properties and potential applications. Molecules. 2004;9:253-266. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022 PMid:15246235
6. Baser KHC, Buchbauer G. Handbook of Essential Oils: Science, Technology, and Applications. CRC Press; 2010. https://doi.org/10.1201/9781420063165
7. Oussalah M et al. Antimicrobial effects of selected plant essential oils. Journal of Applied Microbiology. 2007;102:1236-1245
8. Diop M. Formulation d'un savon transparent antibactérien à base d'huile essentielle. Communication présentée au congrès SOAPGI; 2023. https://doi.org/10.57220/jatpb.v2i3.71
9. Warra AA. Physicochemical analysis of soap produced from different oils. J Chem Pharm Res. 2013;5(7):1-7.
10. Zoller U. Handbook of Detergents: Part A - Properties. Marcel Dekker; 2001.
11. Carroll KC, Pfaller MA, Landry ML, et al. Manual of Clinical Microbiology. 12th ed. Washington DC: ASM Press; 2019. https://doi.org/10.1128/9781555819842
12. World Health Organization. WHO Guidelines on Hand Hygiene in Health Care. Geneva: WHO Press; 2009.
13. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. CLSI document M100. Wayne, PA; 2021.
14. Lambers H, Piessens S, Bloem A, Pronk H, Finkel P. Natural skin surface pH is on average below 5, which is beneficial for its residentflora. International Journal of Cosmetic Science. 2006;28:359-370. https://doi.org/10.1111/j.1467-2494.2006.00344.x PMid:18489300
15. Onawunmi GO. Evaluation of the antimicrobial activity of citral. Phytotherapy Research. 1989;3(4):149-152. https://doi.org/10.1111/j.1472-765X.1989.tb00301.x
16. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of microorganisms. Journal of Applied Microbiology. 2007;102:1236-1245.
17. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils - A review. Food and Chemical Toxicology. 2008;46:446-475. https://doi.org/10.1016/j.fct.2007.09.106 PMid:17996351
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Copyright (c) 2026 Moussa Diop , Amadou Diop , Saliou Khouma, Mor Fall , Djiby Faye , Magatte Cissé , Marie Jeanne Arlette Ciss , Sidy Mouhamed Dieng
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