Pharmacognostic, Physicochemical, Phytochemical Evaluation and Thin Layer Chromatography of Momordica charantia Linn. Fruits

Authors

Abstract

Momordica charantia Linn, well known as bitter gourd belongs to Family Cucurbitaceae. The aim of the present study is to obtained detailed pharmacognostic, physicochemical characters and phytochemical screening of the fruits. The various pharmacognostic parameters were determined viz macroscopic, microscopic including transverse section of the fruit, powder microscopic characteristics, fluorescence analysis, physicochemical properties (Total Ash 7.3 ± 0.11 , acid-insoluble ash 1.40 ± 0.05, water soluble ash 3.16 ± 0.35,alcohol soluble extractive value 8.90 ± 0.11, water soluble extractive value 25.93 ± 0.2, moisture content 17.93 ± 0.08 ) and phytochemical screening of fruits ethanolic extracts reveals the  presence of alkaloids, terpenoids, flavonoids, saponins, tannins & reducing sugars. Rf value was found to be 0.20, 0.27, 0.32, 0.48, 0.54, 0.59, 0.72, 0.85 & 0.93. The current study reveals the standardised parameters for pharmacognostics, physicochemical parameters, phytochemical characteristics, and chromatographic studies of the fruits of Mordica charantia.

Keywords: Fluorescence analysis, Physicochemical, Thin layer chromatography

Keywords:

Fluorescence analysis, Physicochemical, Thin layer chromatography

DOI

https://doi.org/10.22270/jddt.v16i3.7595

Author Biographies

Brij Raj Singh, Department of Pharmacognosy, Malti Memorial Trust CSM Group of Institutions, Faculty of B.Pharmacy, 8th Mile stone Rewa Road, Prayagraj 212111, U.P., India

Department of Pharmacognosy, Malti Memorial Trust CSM Group of Institutions, Faculty of B.Pharmacy, 8th Mile stone Rewa Road, Prayagraj 212111, U.P., India

Amita Verma, Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Bioorganic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, 211007, India

Rajib Kr. Singh, Shakti College of Pharmacy, Balrampur, U.P., India

Professor, Shakti College of Pharmacy, Balrampur, U.P., India

Amit Kumar Singh, United Institute of Pharmacy , UCER, Naini, Prayagraj

Assistant Professor, United Institute of Pharmacy, Naini, Prayagraj, U.P.-211010

References

1. Bharathi, L.K., & John, K.J.," Momordica genus in Asia - An Overview" Springer Nature, 2013. https://doi.org/10.1007/978-81-322-1032-0

2. Grover JK, Yadav SP, "Pharmacological actions and potential uses of Momordica charantia: a review" J Ethnopharmacol, 2004; Jul; 93(1):123-32. https://doi.org/10.1016/j.jep.2004.03.035 PMid:15182917

3. Raman A, Lau C, "Anti-diabetic properties and phytochemistry of Momordica charantia L. (Cucurbitaceae)" Phytomedicine, 1996; Mar; 2(4):349-62. https://doi.org/10.1016/S0944-7113(96)80080-8 PMid:23194773

4. Bailey, C.J., Day, C, Leather dale, B.A., "Traditional treatments for diabetes from Asia & the West Indies" Pract. Diabetes, 1986; 3:190-192. https://doi.org/10.1002/pdi.1960030406

5. Dans AM, Villarruz MV, Jimeno CA, Javelosa MA, Chua J, Bautista R, Velez GG," The effect of Momordica charantia capsule preparation on glycemic control in type 2 diabetes mellitus needs further studies" J Clin Epidemiol, 2007; Jun; 60(6):554-9. https://doi.org/10.1016/j.jclinepi.2006.07.009 PMid:17493509

6. Trease, G.E. and Evans, W.C. Pharmacognosy. 15th ed. London: Saunders Publishers; 2002.

7. Khandelwal. Practical Pharmacognosy.1st ed. Delhi: Nirali Publications; 1995.

8. World Health Organization.Quality control Methods for Medicinal Plant Materials. Delhi: A.I.T.B.S.Publishers; 1998.

9. Indian Pharmacopoeia.Vol. II: 1996, Appendix 3.23, A47.

10. Harborne JB. Phytochemical Methods. 2nd ed; London: Chapman and Hall; 1984 https://doi.org/10.1007/978-94-009-5570-7

11. Vogel AI. A text book of Macro and semi micro qualitatative inorganic analysis. London: Longman Green & Co. Ltd.; 1953.P. 489 -563.

12. Turner RA. Screening Methods in Pharmacology. New York: Academic press; 1965.P. 100-116. https://doi.org/10.1016/B978-1-4832-3266-9.50012-8

13. Stahl Engon. Apparatus and General Techniques in TLC. Thin layer chromatography edited by Egon Stahl. London: George Allen & Unwin Ltd; 1969. P. 52-86. https://doi.org/10.1007/978-3-642-88488-7_3

14. Wagner H, Bladet S, Zgainski EM. Plant Drug Analysis, A TLC Atlas.1st ed. New York; Springer Verlag Berlin Heidelberg; 1994.

15. Wallis TE. Textbook of Pharmacognosy. 5th ed. New Delhi: CBS Publishers & Distributors; 1985.

Published

2026-03-15
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How to Cite

1.
Singh BR, Verma A, Singh RK, Singh AK. Pharmacognostic, Physicochemical, Phytochemical Evaluation and Thin Layer Chromatography of Momordica charantia Linn. Fruits. J. Drug Delivery Ther. [Internet]. 2026 Mar. 15 [cited 2026 Apr. 18];16(3):14-8. Available from: https://www.jddtonline.info/index.php/jddt/article/view/7595

How to Cite

1.
Singh BR, Verma A, Singh RK, Singh AK. Pharmacognostic, Physicochemical, Phytochemical Evaluation and Thin Layer Chromatography of Momordica charantia Linn. Fruits. J. Drug Delivery Ther. [Internet]. 2026 Mar. 15 [cited 2026 Apr. 18];16(3):14-8. Available from: https://www.jddtonline.info/index.php/jddt/article/view/7595

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