Exploring the Therapeutic Potential of Mammillaria beneckei C. Ehrenb. Stem Extracts: In-Vitro Anti-diabetic and Anti-inflammatory Activities
Abstract
Background: The present study investigates the In-Vitro Anti-inflammatory and Anti-diabetic properties of different solvent extracts of Mammillaria beneckei C. Ehrenb. Stem, this cactus species is traditionally used in folk medicine. It belongs to the family Cactaceae and the genus Mammillaria, which is known for its globular or cylindrical shape, and most importantly, its flowers.
Methods: Firstly, Phytochemical screening is required to establish the work. After performing phytochemical screening, many of the compound I have already found like- Alkaloids, Flavonoids, Glycosides, and Tannins etc., Pet. Ether, Methanol, Chloroform, and aqueous extracts were prepared using standard extraction techniques. Anti-inflammatory activity was evaluated through inhibition of albumin denaturation assays, while Anti-diabetic potential was assessed via α-amylase enzyme inhibition methods.
Result & Discussion: All extracts exhibited dose-dependent biological activities, with the methanol extract showing the highest inhibition in both anti-inflammatory and anti-diabetic assays. Phytochemical screening revealed the presence of bioactive compounds such as flavonoids, alkaloids, tannins, and saponins, which may contribute to the observed pharmacological effects.
Conclusion: These findings suggest that Mammillaria beneckei stem extracts possess significant Anti-inflammatory and Anti-diabetic potential, warranting further investigation for their use in the development of natural therapeutic agents. Further studies are warranted to explore its bioactive compounds and their mechanisms of action.
Keywords: Cactaceae, Albumin denaturation, Bioactive compounds, Phytochemical screening.
Keywords:
Cactaceae, Albumin denaturation, Bioactive compounds, Phytochemical screeningDOI
https://doi.org/10.22270/jddt.v15i6.7220References
1. Oome, S., Variable cotyledon numbers in Mammillaria beneckei (Cactaceae). 2005.
2. Harpke, D., et al., Phylogenetic evaluation of chloroplast trnL-trnF DNA sequence variation in the genus Mammillaria (Cactaceae). Schlechtendalia, 2006;14:7-16.
3. Breslin, P.B., M.F. Wojciechowski, and L.C. Majure, Molecular phylogeny of the Mammilloid clade (Cactaceae) resolves the monophyly of Mammillaria. Taxon, 2021;70(2):308-323. https://doi.org/10.1002/tax.12451
4. Cervantes, C.R., et al., Evaluating the monophyly of Mammillaria series Supertextae (Cactaceae). PhytoKeys, 2021;177:25. https://doi.org/10.3897/phytokeys.177.62915 PMid:33967580 PMCid:PMC8099837
5. Dewanjee, S., et al., Study of anti-inflammatory and antinociceptive activity of hydroalcoholic extract of Schima wallichii bark. Pharmaceutical Biology, 2009;47(5):402-407. https://doi.org/10.1080/13880200902758824
6. Das, M.J. and B.R. Sarkar, Evaluation of in vitro anti-inflammatory activity of methanolic extract of Impatiens bracteata Colebr. ex wall.
7. Akbar, S., et al., Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pacific journal of tropical biomedicine, 2014;4(5):410-415. https://doi.org/10.12980/APJTB.4.2014C1107 PMid:25182728 PMCid:PMC3985058
8. Alam, T., et al., Pharmacognostical parameters, sphytochemical screening, TPC, TFC and evaluation of in-vitro anti-oxidant activity of leaves of Cymbidium aloifolium L (SW). Research Journal of Pharmacy and Technology, 2024;17(12):5743-5749. https://doi.org/10.52711/0974-360X.2024.00874
9. Osei Akoto, C., et al., Anthelminthic, Anti‐Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem‐Bark. Journal of Chemistry, 2021;2021(1):3328073. https://doi.org/10.1155/2021/3328073
10. Akoto, C.O., et al., In vitro anthelmintic, anti-inflammatory, antioxidant activities and FTIR analysis of Sclerocarya birrea root. 2020.
11. Osei Akoto, C., et al., Anti‐Inflammatory, Antioxidant, and Anthelmintic Activities of Ocimum basilicum (Sweet Basil) Fruits. Journal of Chemistry, 2020;2020(1):2153534. https://doi.org/10.1155/2020/2153534
12. Bagchi, A., B.R. Sarkar, and P. Pal, An antidiabetic and antioxidant activity of an unexplored plant of Diapensia himalaica in Alloxan-induced diabetic rats, African Journal of Biological Sciences. 2024;6(5):1646-1658
13. Chakraborty, R., S. Roy, and V. Mandal, Assessment of traditional knowledge of the antidiabetic plants of Darjeeling and Sikkim Himalayas in the context of recent phytochemical and pharmacological advances. Journal of Integrative Medicine, 2016;14(5):336-358. https://doi.org/10.1016/S2095-4964(16)60267-4 PMid:27641606
14. Sarkar, B.R., Phytochemical Screening and Proximate exploration of Diapensia himalacia leaf extract.
15. Azab, A., A. Nassar, and A.N. Azab, Anti-inflammatory activity of natural products. Molecules, 2016;21(10):1321. https://doi.org/10.3390/molecules21101321 PMid:27706084 PMCid:PMC6274146
16. Vallianou, N.G., et al., Honey and its anti-inflammatory, anti-bacterial and anti-oxidant properties. Gen Med (Los Angel), 2014;2(132):1-5. https://doi.org/10.4172/2327-5146.1000132
17. Chirumamilla, P. and S. Taduri, Assessment of in vitro anti-inflammatory, antioxidant and antidiabetic activities of Solanum khasianum Clarke. Vegetos, 2023;36(2):575-582. https://doi.org/10.1007/s42535-022-00410-6
18. Singh, A.K., et al., Evaluation of antidiabetic activity of dietary phenolic compound chlorogenic acid in streptozotocin induced diabetic rats: Molecular docking, molecular dynamics, in silico toxicity, in vitro and in vivo studies. Computers in biology and medicine, 2021;134:104462. https://doi.org/10.1016/j.compbiomed.2021.104462 PMid:34148008
19. Singh, K.K., S.S. Singh, and C.L. Sharma, Antidiabetic and antioxidant properties of important traditional medicinal plants from Northeastern India. Int J Pharm Sci & Res, 2024;15(6):1728-36.
20. Ginwala, R., et al., Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin. Antioxidants, 2019;8(2):35. https://doi.org/10.3390/antiox8020035 PMid:30764536 PMCid:PMC6407021
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