Investigation of the therapeutic effect of in vitro Ceratonia siliqua application on DNA damage caused by human sperm cryopreservation
Abstract
Objectives: The main aim of this study was to investigate the effect of Ceratonia siliqua L (carob) plant added to the culture medium to reduce the negative effect on sperm parameters as a result of cryodamage occurring in sperm during human sperm cryopreservation.
Materials and methods: Semen samples obtained with consent from 50 infertile men aged 25-40 years were subjected to spermiogram test. Spermatozoa were divided into normospermia and asthenospermia. Semen was washed with gradient method to separate spermatozoa according to motility and vitality with the least risk of cotamination. The groups were divided into 6 groups. normospermic group with gradient method, normozoospermic group with gradient method and freeze-thawed, normozoospermic group with gradient method and freeze-thawed and Ceratonia siliqua L., normozoospermic group with only gradient method, asthenozoospermic group with gradient method, asthenozoospermic group with gradient method and freeze-thawed, asthenozoospermic group with gradient method and freeze-thawed and added Ceratonia siliqua L. Semen samples were frozen at -196 °C with liquid nitrogen and stored in a nitrogen tank. Then the samples were thawed at 37 °C and vitality test was performed with eosin Y stain in all groups.
Results: In order to investigate the effect of freeze-thawing on sperm parameters, only semen samples washed by gradient method were compared with the frozen and thawed group without the addition of Ceratonia siliqua L. Cryopreservation had a significant (p˂0.05) negative effect on all sperm parameters. In the investigation of the effect of Ceratonia siliqua L. on cryodamage, sperm vitality parameter was compared in the groups in which Ceratonia siliqua L. was added to the incubation medium in vitro. Ceratonia siliqua L. concentration was adjusted as 0.01%. A positive difference was observed between the groups to which Ceratonia siliqua L. was added compared to the groups to which Ceratonia siliqua L. was not added.
Conclusion: According to the results of this study, the negative effects of sperm freezing on sperm vitality are clearly demonstrated. Considering the positive differences in the groups in which Ceratonia siliqua L. was added in vitro, we think that Ceratonia siliqua L. will contribute to male infertility.
Keywords:
sperm, freezing, vitality, Ceratonia siliqua LDOI
https://doi.org/10.22270/jddt.v14i12.6906References
1. Singh, R., Hamada, A. J., Bukavina, L., & Agarwal, A. Physical deformities relevant to male infertility. Nature reviews. Urology, 2012; 9(3):156-174. https://doi.org/10.1038/nrurol.2012.11 PMid:22349654
2. Tous, J., Romero, A., Battle, I., The carob tree: botany, horticulture, and genetic resources. Horticultural Reviews, 2013; 41:385-456. https://doi.org/10.1002/9781118707418.ch08
3. Kumazawa, S., Taniguchi, M., Suzuki, Y., Shimura, M., Kwon, M., Nakayama, T. Antioxidant activity of polyphenols in carob. Journal of Agriculture and Food Chemistry, 2002; 50:373- 377. https://doi.org/10.1021/jf010938r PMid:11782210
4. Owen, R.W., Haubner, R., Hull, W.E., Erben, G., Spiegelhalder, B., Bartsch, H. Isolation and elucidation of the major individual polyphenols in carob fibre. Food and Chemical Toxicology,2003; 41:1727-1738. https://doi.org/10.1016/S0278-6915(03)00200-X PMid:14563398
5. Ahmed, M.M., Biochemical studies on nephroprotective effect of carob (Ceratonia siliqua L.) growing in Egypt. Nature and Science,2010, 8(3), 41- 47.
6. WHO, 2010. Laboratory manual for the examination of human semen and sperm cervical mucus interaction Cambridge University Press p.
7. Yusuf, M.K., Ibegbu, A.O., Agbon, A.N., Evang, A., Determination of Median Lethal Dose of Ethanolic Extract of Sesamum indicum seeds in adult Wistar Rats., African Journal of Cellular Pathology, 2017; 9:73-76. https://doi.org/10.5897/AJCPath2018.0011
8. Altun R, Özden A. Complementary and alternative medicine.; Güncel Gastroenteroloji 2004;8(3):231-235.
9. Zini A, Fischer MA, Nam RK, Jarvı K. Use of alternatve and hormonal therapies in male infertility.; Urology 2004;63 (1):141-143. https://doi.org/10.1016/j.urology.2003.07.018 PMid:14751367
10. Fraser L, Dziekońska A, Strzeżek R, Strzeżek J, Dialysis of boar semen prior to freezing-thawing: Its effects on post-thaw sperm characteristics. Theriogenology, 2007; 67(5):994-1003. https://doi.org/10.1016/j.theriogenology.2006.12.002 PMid:17218000
11. Condorelli, R.A., La Vignera, S., Di Bari, F., Unfer, V., Calogero, A.E., Effects of myoinositol on sperm mitochondrial function in vitro. Eur Rev Med Pharmacol Sci, 2011; 15(2):129-134.
12. Condorelli, R.A., La Vignera, S., Bellanca, S., Vicari, E., Calogero, A.E., Myoinositol: does it improve sperm mitochondrial function and sperm motility?. J Urol, 2012; 79(6):1290-1295. https://doi.org/10.1016/j.urology.2012.03.005 PMid:22656408
13. Türk, S., Mändar, R., Mahlapuu, R., Viitak, A., Punab, M., Kullisaar, T., Male infertility: decreased levels of selenium, zinc and antioxidants. J Trace Elem Med Biol, 2014; 28(2):179-185. https://doi.org/10.1016/j.jtemb.2013.12.005 PMid:24462254
14. Mendiola, J., Torres-Cantero, A.M., Vioque, J., Moreno-Grau, J.M., Ten, J., Roca, M. A low intake of antioxidant nutrients is associated with poor semen quality in patients attending fertility clinics. Fertil Steril, 2010; 93(4):1128-1133. https://doi.org/10.1016/j.fertnstert.2008.10.075 PMid:19147135
Published
Abstract Display: 174 
PDF Downloads: 101 PDF Downloads: 10 How to Cite
Issue
Section
Copyright (c) 2024 Dilara Akıncı , Murat Akkuş , Zeynep Türe , Gül Ebru Aydeniz Acar , Mehmet Ölmez , Esma Yıldırım , Eda Yıldızhan , Mehmet Emin Akıncı
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.