Evaluation of Anti-Bacterial Activity of Turnip Green Leaves Extracts
Abstract
The study aimed to assess the antimicrobial potential of aqueous and methanolic extracts from Brassica rapa L. leaves. Phytochemical analysis revealed the presence of various compounds including carbohydrates, fats, proteins, vitamins, minerals, electrolytes, sugars, soluble and insoluble fiber, and amino acids. Both extracts were evaluated for antibacterial activity against six microorganisms, including gram-positive and gram-negative bacteria, compared with the standard antibiotic Gentamycin. Methanolic extract exhibited higher antibacterial efficacy against gram-positive bacteria compared to the aqueous extract.
Although specific active components were not isolated, the plant extracts contained potential antibacterial agents such as soluble fiber, minerals, amino acids, isothiocyanate glycosides, and essential oils. Additionally, compounds like 3,3'-diindolylmethane, sulforaphane, selenium, indole-3-carbinol, and vitamins like C, E, A, and K were identified. The methanolic extract, rich in sulfur and nitrogen-containing compounds, showed promising antibacterial activity against gram-positive bacteria.
In conclusion, Brassica rapa L. leaves could serve as a valuable source for developing safe antibacterial agents. The observed spectrum of activity suggests the potential for further exploration in drug development.
Keywords: Brassica rapa L., antibacterial activities, agar disc diffusion method, agar well diffusion method, Gentamycin, phytochemical analysis, Turnip green
Keywords:
Brassica rapa L., antibacterial activities, agar disc diffusion method, agar well diffusion method, phytochemical analysis, Turnip greenDOI
https://doi.org/10.22270/jddt.v14i12.6554References
1. Shetty K, Wahlqvist ML. Phytonutrient sources from vegetables and fruits. In: Phytochemicals. Springer, Boston, MA (2004).
2. Sharifi-Rad J, Mnayer D, Morais-Braga MF, et al. Evaluating the antimicrobial potential of green synthesized nanoparticles for sustainable applications in food safety. Food Control (2018)., 89, 245-253.
3. Silva-Beltrán NP, Ruiz-Cruz S, Cira-Chávez LA, et al. Plant extracts: From encapsulation to application. In: Plant Extracts: Encapsulation, Preservation and Application. John Wiley & Sons, Ltd. (2017)
4. Dejanovic GM, Asllanaj E, Gamba M, et al. Phytochemical characterization of turnip greens (Brassica rapa ssp. rapa): A systematic review. PLoS One. 2021;16(2 February):1-16. https://doi.org/10.1371/journal.pone.0247032
PMid:33596258 PMCid:PMC7888597
5. Cao Q, Wang G, Peng Y. A Critical Review on Phytochemical Profile and Biological Effects of Turnip (Brassica rapa L.). Front Nutr. 2021;8(July):1-6. https://doi.org/10.3389/fnut.2021.721733
PMid:34395503 PMCid:PMC8360391
6. Bonilla J, Atarés L, Vargas M, Chiralt A. Edible films and coatings to prevent the detrimental effect of oxygen on food quality: Possibilities and limitations. Journal of Food Engineering, (2012) 110(2), 208-213. https://doi.org/10.1016/j.jfoodeng.2011.05.034
7. Handa SS. An Overview of Extraction Techniques for Medicinal and Aromatic Plants. Extraction Technologies For Medicinal And Aromatic Plants, United Nations Industrial Development Organization and The International Centre For Science and High Technology: Italy,. Published online 2008; p 11.
8. Farnsworth, N.R. Biological approaches to the screening and evaluation of natural products. In: Rasoanaivo P, Ratsimamanga-Urverg S (Eds) Biological Evaluation of Plants with Reference to the Malagasy Flora, Madagascar, (1993): pp. 35-43.
9. Romero, C.D., Chopin, S.F., Buck, G., Martinez, E., Garcia, M., Bixby, L. Antibacterial properties of common herbal remedies of the southwest. J. Ethnopharmacol. (2005):99: 253-257. https://doi.org/10.1016/j.jep.2005.02.028
PMid:15894135
10. Lin, J., Opoku, A.R., Geheeb-Keller, M., Hutchings, A.D., Terblanche, S.E., Jager, A.K., van Staden, J. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and Antimicrobial activities. J Ethnopharmacol. (1999): 68: 267-2. https://doi.org/10.1016/S0378-8741(99)00130-0
PMid:10624887
11. Yao, J., Moellering, R. Antibacterial agents. In: Manual of Clinical Microbiology, Murray P, Baron E, Pfaller M, Tenover F, Yolken R (Eds), ASM, Washington DC, (1995): pp.1281-1290.
12. Cetin, T.E., Gurler, N. Bakterilerin antibiyotiklere duyaarlilik deneyinin yapilmasi. Kukem Dergisi, (1989):12: 2-5.
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Copyright (c) 2024 Jagwinder Kaur, Meenakshi Malhotra , Ajeet Pal Singh , Amar Pal Singh

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