Phytochemical, GC-MS, FTIR and Amino acid profile of methanol extract of Tetrapleura tetraptera fruit
Abstract
The methanolic fruit extract of Tetrapleura tetraptera was analyzed for the presence of phytocompounds, their bioactivity, the functional groups involved in this activity, and its amino acid profile using standard procedures. Phytochemicals such as tannins, phenols, flavonoids, and alkaloids were identified as being highly present. Gas chromatographic-mass spectrometric (GC-MS) analysis identified 16 bioactive compounds, with 2-thiopheneethanol (58.77%) being the most abundant. Curcumin, with the most diverse pharmacological role, and other bioactive compounds such as cedren-13-ol, 8 (1.56%), N-benzyl stearamide (4.46%), a prominent fatty acid amide hydrolase (FAAH) inhibitor; pthalic acid, butyl undecyl ester (1.49%); and phenol, 2, 6-bis (1,1 dimethyl ethyl) (1.46%), were also present. Fourier transform infrared (FTIR) analysis confirmed the presence of alkanes, esters, benzene rings, aliphatic, sulfonic acid, and methylene chains. Also, the amino acid analysis of the T. tetraptera revealed that the fruit contains 18 amino acids. Leucine (4.20%), phenylalanine (3.37%), and valine (3.25%) were the most abundant essential amino acids identified, with glutamic (7.20%) and aspartic acid (5.61%) having the highest concentrations as non-essential amino acids. This therefore indicates that T. tetraptera fruit could be used as a pharmacological or therapeutic agent as well as a dietary condiment, particularly at this time when there is a demand for novel protein sources.
Keywords: Tetrapleura tetraptera, phytochemicals, GC-MS, FTIR, amino acid, curcumin
Keywords:
Tetrapleura tetraptera, phytochemicals, GC-MS, FTIR, amino acid, curcuminDOI
https://doi.org/10.22270/jddt.v13i2.5739References
Mohan N, Jassal PS and Singh RP. Comparative In vitro and In vivo study of antioxidants and phytochemical content in Bacopa monnieri. Rcent Research in Science and Technology. 2011; 3(9):78-83
Ngozi AI. Phytomedicines and Nutraceuticals: Alternative Therapeutics for Sickle Cell Anemia", The Scientific World Journal, 2013, 12pp. https://doi.org/10.1155/2013/269659
Akerele O. Importance of medicinal plants: WHO’s programme. In: Natural Resources and Human Health: Plants of medicinal and nutritional value. Elsevier, Amserdam, Netherlands, 1992; 63-77
Keke CO, Nsofor WN, Kumabia FKR, Iloabuchi GC, Ejiofor JC, Osuagwu OL. GCMS and FTIR analysis of ethanol and methanol leave extract of Urena lobata (Caesar weed) for bioactive phytochemical constituents. JDDT 2023; 13(1):99-115. https://doi.org/10.22270/jddt.v13i1.5722
Enema OJ, Adesina SK, Umoh UF, and Eseyin OA. Gas chromatography-mass spectroscopy (GC-MS) studies of fixed oil of leaf of Tetrapleura tetraptera Taub. (Mimosaceae). Journal of Pharmacognosy and Phytochemistry. 2019; 8(6):1237-124.
Oriolowo OB, John OB, Mohammed UB and Joshua D. Amino acid profile of catfish, crayfish and larva of edible dung beetle. Ife Journal of Science. 2020. 22(1). https://doi.org/10.4314/ijs.v22i1.2
Oni JO, Akomaye FA, Uman EJ and Damian AO. Amino acid profiles and bioactive compounds of four inedible mushrooms from Oban Division Cross River National Park (CRNP), Nigeria. European Journal of Biology and Biotechnology. 2021; 2(2):11-18 https://doi.org/10.24018/ejbio.2021.2.2.157
Adesina SK, Iwalewa EO, and Johnny II. Tetrapleura tetraptera Taub- Ethnopharmacology, Chemistry, Medicinal and Nutritional Values-A Review. British Journal of Pharmaceutical Research. 2016; 12(3):1-22. https://doi:10.9734/BJPR/2016/26554
Nwoba EG. Proximate and phytochemical composition of the pulp of Tetrapleura tetraptera fruits consumed in Abakaliki, Nigeria. Int.J.Eng.Res. Technol. 2015, 4:10-20. https://doi:10.17577/IJERTV4IS060622
Kemigisha E, Owusu EO, Elusiyan CA, Omujal F, Tweheyo M, and Bosu PP. Tetrapleura tetraptera in Ghana, Nigeria and Uganda: households’ uses and local market. Forests, Trees and Livelihoods. 2018. http://hdl.handle.net/123456789/745
N’zebo NJ, Ahi AP, Dje KM, Kabran AF and Kouamé LP. Chemical Composition and mineral bioavailability of Tetrapleura tetraptera (Schumach & Thonn.) Taub. Fruit Pulp Consumed as Spice in South-eastern Côte d’Ivoire. Turkish Journal of Agriculture - Food Science and Technology. 2019; 7(11):1817-1824. https://doi.org/10.24925/turjaf.v7i11.1817-1824.2662
Ogbunugafor H, Ugochukwu C, and Kyrian-Ogbonna E., The role of spices in nutrition and health: A review of three popular spices in Southern Nigeria. Food Quality and Safety. 2017; 1(3):1-15. https://doi.org/10.1093/fqsafe/fyx020
Ncube NS, Afolayan AJ, and Okoh, AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology. 2008; 7(12):1797-1806. https://doi.org/10.5897/AJB07.613
Adegoke AA, IberI PA, Akinpelu DA , Aiyegoro OA and Mboto CI. Studies on phytochemical screening and antimicrobial potentials of Phyllanthus amarus against multiple antibiotic resistant bacteria. Int. J. Appl. Res. Nat. Prod.2010; 3(3):6-12. https://doi:10.163.pcbsj/2022.16.‐1‐57
Trease GE and Evans WC. Pharmacognosy. 15th Ed. Saunders Publishers, London. 2002. pp 214-393
Adeyeye EI and Afolabi EO. Amino acid composition of three different types of land snails consumed in Nigeria. Food Chemistry. 2004; 85:534-539. http://dx.doi.org/10.1016%2FS0308-8146(03)00247-4
Maria MY, Justo P, Julio G, Javier V, Francisco M. and Manuel A. Determination of tryptophan by high-performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection. Food Chemistry 2004. 85(2):317-320. https://doi.org/10.1016/j.foodchem.2003.07.026
Adnan Md, Oh, KK, Azad MOK, Shin MH, Wang M-H and Cho DH. Kenaf (Hibiscus cannabinus L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties. Life 2020; (10):223. https://doi.org/10.3390/life10100223
Alasmari M, Bӧhlke M, Kelley C, Maher T, Pino-Figueroa A. Inhibition of fatty acid amide hydrolase (FAAH) by macamides. Molecular neurobiology. 2018; 56(3):1770-81. https://doi:10.1007/s12035-018-1115-8
Begum F, Mohankumar R, Jeevan M and Ramani K. GC–MS Analysis of Bio-active Molecules Derived from Paracoccuspantotrophus FMR19 and the Antimicrobial Activity Against Bacterial Pathogens and MDROs. Indian J Microbiol. 2016; 56(4):426-432. https://doi:10.1007/s12088-016-0609-1
Peng HV, Lai CC, Lin CC and Chou ST. Effects of Vetiveria zizanioides essential oil on melanogenesis in melanoma cells: down regulation of tyrosinase expression and suppression of oxidative stress. The Scientific World Journal. 2014. 213013. https://doi:10.1155/2014/213013
Perrone D, Ardito F, Giannatempo G, Dioguard M, Troiano G, Russo LL, De Lillo A, Laino L and Muzio LL. Biological and therapeutic activities, and anticancer properties of curcumin Review. Experimental and Therapeutic Medicine. 2015; 1615-1623. https://doi:10.3892/etm.2015.2749
Egorova KS, Gordeev EG and Ananikov VP. Biological Activity of Ionic Liquids and Their Application in Pharmaceutics and Medicine. Chem. Rev. 2017; (117)10:7132–7189. https://doi:10.1021/acs.chemrev.6b00562 .
Al-Gara’awi N, Abu-Serag NA, Saheed KAA, Bahadly ZKA. Analysis of bioactive phytochemical compound of (Cyperusalternifolius L.) By using gas chromatography –mass spectrometry. ICCEPS IOP Conf. Series: Materials Science and Engineering. 2019; 571. IOP Publishing https://doi:10.1088/1757-899X/571/1/0120471
Phillips S, Rao MRK, Prabhu K, Priya M, Kalaivani S, Ravi A and Dinakar, S. Preliminary GC-MS analysis of an Ayurvedic medicine “KulathadiKashayam” Journal of Chemical and Pharmaceutical Research. 2015; 7(9):393-400.
Costantino L, Parenti C, Di Bella M, Zanoli P, Baraldi M. Anti-inflammatory activity of newly synthesized 2, 6-bis-(1, 1-dimethylethyl) phenol derivatives. Pharmacological research. 1993; 27(4):349-58. https://doi:10.1006/phrs.1993.1034 .
Larbie C, Mills-Robertson FC, Opoku RA, Kabiri NC and Abrokwah RO. Tetrapleura tetraptera of Ghanaian Origin: Phytochemistry, Antioxidant and Antimicrobial Activity of Extracts of Plant Parts. Journal of Pharmaceutical Research International. 2020; 32(35):78-96. https://doi.org/10.9734/jpri/2020/v32i3530981
Ezeonu S and Ejikeme C. Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods. Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods. 2016. 9. https://doi:10.1155/2016/5601327 .
Deplano A, Cipriano M, Moraca F, Novellino E, Catalanotti B, Fowler CJ, Onnis V. Benzylamides and piperazinoarylamides of ibuprofen as fatty acid amide hydrolase inhibitors. Journal of enzyme inhibition and medicinal chemistry. 2019; 34(1):562-76. https://doi.org/10.1080/14756366.2018.1532418
Raja Rajeswari N, RamaLakshmi S, Muthuchelian K. GC-MS analysis of bioactive components from the ethanolic leaf extract of Canthiumdicoccum (Gaertn.) Teijsm&Binn. J Chem Pharm Res. 2011; 3(3):792-8.
Tepe B, Akpulat HA, Sokmen M. Evaluation of the chemical composition and antioxidant activity of the essential oils of Peucedanum longifolium (Waldst. & Kit.) and P. palimbioides (Boiss.). Records of natural products. 2011; 5(2):108. https://doi.org/10.1016/j.jfoodeng.2004.04.015
Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases.The international journal of biochemistry & cell biology. 2009; 41(1):40-59. https://doi:10.1016/j.biocel.2008.06.010
Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cellular and molecular life sciences. 2008; 65(11):1631-52. https://doi:10.1007/s00018-008-7452-4 .
Tu CX, Lin M, Lu SS, Qi XY, Zhang RX, Zhang YY. Curcumin inhibits melanogenesis in human melanocytes. Phytotherapy Research. 2012; 26(2):174-179. https://doi:10.1002/ptr.3517 .
Hu AP, Liu YL, Shi LK. Widespread occurrence of phthalic acid esters in raw oilseeds in China used for edible vegetable oil production. Food Additives & Contaminants: Part A. 2016; 33(9):1421-7. https://doi:10.1080/19440049.2016.1222631
Rajamanikyam M, Vadlapudi V, Parvathaneni SP, Koude D, Sripadi P, Misra S, Amanchy R, Upadhyayula SM. Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest. EXCLI journal. 2017; 16, 375. http://dx.doi.org/10.17179/excli2017-145
Aboobaker Z, Viljoen A, Chen W, Crous PW, Maharaj VJ, Van Vuuren S. Endophytic fungi isolated from Pelargonium sidoides DC: antimicrobial interaction and isolation of a bioactive compound. South African Journal of Botany. 2019; 122:535-542. https://doi.org/10.1016/j.sajb.2019.01.011
Amade P, Mallea M, Bouaicha N. Isolation, structural identification and biological activity of two metabolites produced by Penicillium olsonii Bainier and Sartory. The Journal of Antibiotics. 1994; 47(2):01-08. https://doi:10.7164/antibiotics.47.201
Dr. Duke’s. Phytochemical and Ethnobotanical Databases,Phytochemical and Ethnobotanical, Databases.www.ars-gov/cgi-bin/duke/. 2020.
Al–Khafaji SA. Determination of The Anti-Fungal Effort of Morganella Morganii and Determination of Its Chemical Composition by Means of Gas Chromatography Method-Mass Spectrometry Gas Chromatography-Mass Spectrometry. Journal of University of Babylon for Pure and Applied Sciences. 2018; 26(5):295-308.
Hamza LF, Kamal SA, Hameed IH. Determination of metabolites products by Penicillium expansum and evaluating antimicobial activity. Journal of Pharmacognosy and Phytotherapy. 2015; 7(9):194-220. https://doi.org/10.5897/JPP2015.0360
Mensah-Agyei, G. O, Enitan, S.S, Adetiloro, E.O, Ezeamagu, C.O. Identification of Bioactive Compounds in the acetone extract of Daedalea elegans using Gas Chromatography–Mass Spectrometry: A review. Int. J. Med. Phar. Drug Re. 2021:1. http://dx.doi.org/10.22161/ijmpd.5.5.1
Oguoma OI, Ezeifeka GO, Adeleye SA, Oranusi S and Amadi ES. Antimicrobial activity, proximate and amino acid analysis of Tetrapleuratetraptera. Nigerian Journal of Microbiology. 2015; 27(1): 2709-2718.
Abdou Bouba A, Ponka R, Augustin G, Njintang Yanou N, Abul-Hamd El-Sayed M, Montet D, Scher J and Mbofung CM. Amino acid and fatty acid profile of twenty wild plants used as spices in Cameroon. American Journal of Food Science and Technology, 2016; 4 (2):29-37. http://dx.doi.org/10.12691/ajfst-4-2-1
Odukoya JO, Odukoya JO, Mmutlane EM and Ndinteh DT. Phytochemicals and Amino Acids Profiles of Selected sub-Saharan African Medicinal Plants’ Parts Used for Cardiovascular Diseases’ Treatment. Pharmaceutics. 2021; 13(9):1367. https://doi.org/10.3390/pharmaceutics13091367
Moran-Palacio E, Tortoledo O, Yañez G, Zamora-Alvarez L, Stephens-Camacho N, Soñanez-Organis J, Ochoa-Lopez L and Rosas-Rodríguez J. Determination of Amino Acids in Medicinal Plants from Southern Sonora, Mexico. Tropical Journal of Pharmaceutical Research. 2014; 13:601-606. https://doi:10.4314/tjpr.v13i4.17
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