Pharmacognostical, Phytochemical and In-Vitro Anthelmintic Activity of Cassia roxburghii Seed and Boerhaavia diffusa Root against Pheritima posthuma Model
Abstract
Plants have served human beings as a natural source for treatments and therapies from ancient times, amongst them medicinal herbs have gain attention because of its wide use and less side effects. In the recent years plant research has increased throughout the world and a huge amount of evidences have been collected to show immense potential of medicinal plants used in various traditional systems. The objective of this study was to investigate pharmacognostical, phytochemical features and anthelmintic action of Cassia roxburghii (seeds) and Boerhaavia diffusa (roots). The different pharmacognostical parameters were evaluated as per standard protocols with some modifications. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. Phytochemical analysis revealed the presence of alkaloids, glycosides, flavonoids, steroids, saponins, triterpenoits and carbohydrate. Three different doses (10, 25, & 50 mg/ml) of each extracts were studied, which involves the determination of time of paralysis and time of death of worm. All the extracts showed a dose dependent increase in the anthelmintic action. Out of all the extracts, the methanol extracts showed highest activity in both plants followed by ethyl and aqueous extracts. At the concentration of 50 mg/ml the methanol showed a remarkable anthelmintic activity which was even greater than the standard drug (Albendazole) at the same concentration.
Keywords: Cassia roxburghii, Boerhaavia diffusa, Pheretima posthuma, Anthelmintic action, Paralysis, Albendazole.
Keywords:
Cassia roxburghii, Boerhaavia diffusa, Pheretima posthuma, Anthelmintic action, Paralysis, AlbendazoleDOI
https://doi.org/10.22270/jddt.v12i6-S.5711References
Bundy DA. Immunoepidemiology of intestinal helminthic infection I: The global burden of intestinal nematode disease. Trans Royal Soc Trop Med Hyg. 1994; 8:259-261. https://doi.org/10.1016/0035-9203(94)90069-8
Tagbota S, Townson S. Antiparasitic properties of medicinal and other naturally occurring products, Adv Parasitol. 2001; 50:199-205. https://doi.org/10.1016/S0065-308X(01)50032-9
Girhepunje K, Arulkumaran, Pal R, Maski N, Thirumoorthy N. A novel binding agent for pharmaceutical formulation from Cassia roxburghii seeds. Int J Pharm Pharm Sci. 2009; 1:1-5.
Waller PJ., International approaches to the concept of integrated control of nematode parasites of livestock. Int J Parasitol. 1999; 29:155-64. https://doi.org/10.1016/S0020-7519(98)00178-7
Kosalge SB, Fursule RA. Investigation of in vitro anthelmintic activity of Thespesia lampas (Cav.). Asian Journal of Pharmaceutical and Clinical Research. 2009; 2(2):69-71.
Satyavati GV, Raina MK, Sharma M. Medicinal Plants of India. Vol. I, pp: 201-06. Indian Council of Medical Research, New Delhi.1976.
Lewis WH, Elvin- Lewis MPH. Medicinal Botany Plants Affecting Man's Health. John Wiley and Sons, New York.1977.
Maurya SD, Jain D, Porwal O, Dhakar RC, Jayakumararaj R, A Review on Role of Herbal Antioxidants in Periodontitis, Asian Journal of Dental and Health Sciences 2021; 1(1):14-18 https://doi.org/10.22270/ajdhs.v1i1.1
Chopra RN, Nayyar SL, Chopra IC Glossary of Indian Medicinal Plants, 160 pp. Council of Scientific and Industrial Research, New Delhi. 1956.
Akhtar MS, Iqbal Z, Khan MN, Lateef M. Anthelmintic activity of medicinal plants with particular reference to their use in animals in Indo-Pakistan subcontinent. Small Rumin. Res. 2000; 38:99-107. https://doi.org/10.1016/S0921-4488(00)00163-2
Iqbal Z. Nadeem QK, Khan MN, Akhtar MS, Waraich FN. In vitro anthelmintic activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Journal of Agriculture and Biology. 2001; 3(4):454-457.
Hudai Mazumderb M, Mohammad M, Bustanji Y, Tayyem R, Youse F. Ethno pharmacological survey of medicinal plants in Jordan, Mujib Nature reserve and surrounding area. J. Ethnopharmacology. 2008; 120:63-71. https://doi.org/10.1016/j.jep.2008.07.031
Hennebelle T, Weniger B, Joseph H, Sahpaz S, Bailleul F. Senna alata. Fitoterapia 2009; 80:385-393 https://doi.org/10.1016/j.fitote.2009.05.008
http://www. Flowersofindia.net/catalog/slides/Red%20 Cassia.html
Pandita N, Vaidya A. Pharmacognistic and Phytochemical studies of Cassia seed Extract Mumbai 2015.
Rendle AB. The Classification of Flowering Plants: Dicotyledons. Vol.2, Cambridge University Press, London, UK, 1925.
Chopra GL, Angiosperms: Systematics and Life Cycle. S. Nagin & Co., Jalandhar, Punjab, India, 1969, pp. 361-365.
Singh A. Boerhaavia diffusa: An over-exploited plant of medicinal importance in eastern Uttar Pradesh. Current Science 2007; 93(4):446.
Correa MP. Dicionario de Plantas U teis do Brazil e das Exo' ticas Cultivadas. IBDF, Ministe'rio da Agricultura, Rio de Janeiro, 1984; IV:159.
Chauhan M, Pillai APG. Ayurvedic Medicinal Flora of Gujarat state with distribution atlases. 2000; 1:38-39.
Chaudhary G, Dantu PK. Morphology, Phytochemical and Pharmacological, studies on Boerhaavia diffusa" Department of Botany, Faculty of Science, Dayalbagh Educational institute (Deemed University), Dayalbagh Agra India. 2011, vol.5.
Khadagwanshi D, Trivedi S, Patel P, Jha M. Effect of Phaseolus vulgaris on E. coli induced peritonitis and bacteraemia in mice. Journal of Drug Delivery and Therapeutics. 2019; 9(4-s):310-4. https://doi.org/10.22270/jddt.v9i4-s.3323
Dutta R, Sharma MK, Jha M. Pharmacological evaluation of antiasthmatic activity of fumaria officinalis extracts. Plant Archives. 2020; 20(2):4308-15.
Nayak A, Garg M, Jain S, Khan MA, Jain DK, Khan N. Anti-Urolithiatic and invitro-invivo anti-oxidant effects of methanolic extract of thunbergia laurifolia on ethylene glycol-induced kidney calculi in rats. Sch Acad J Pharm. 2019; 8(3):94-104.
Aziz N, Wal P, Wal A, Saxena MS. Evaluation of a Polyherbal Powder for Treatment of Diabetes Mellitus. Indian Journal of Pharmaceutical Sciences. 2019; 81(6):1070-1077. https://doi.org/10.36468/pharmaceutical-sciences.605
Roopashree TS, Dang R, Rani SRH, Narendra C. Antibacterial activity of anti-psoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied Research in Natural Products. 2008; 1(3):20-28.
Ajaiyeoba, EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extracts. Pharmaceutical biology. 2001; 39(3):217-220. https://doi.org/10.1076/phbi.39.3.217.5936
Published
Abstract Display: 456 
PDF Downloads: 490 PDF Downloads: 57 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.