Evaluation of Antioxidant activities of Aqueous extract from Bryophyllum pinnatum on Carbon tetrachloride-induced albino rat tissues
Abstract
Oxidative injury or cellular damage arising from free radicals or reactive oxygen species (ROS) has been implicated in the pathogenesis of many human diseases. These ROS are generated through normal metabolism of drugs, environmental chemicals and other xenobiotics as well as endogenous chemicals. Accumulated evidence suggests that ROS can be scavenged through chemoprevention utilizing natural antioxidant compounds present in foods and medicinal plants. The aim of this study is to evaluate antioxidant activities of aqueous extract of Bryophyllum pinnatum using carbon tetrachloride-induced albino rats as model. An in-vivo antioxidant activity was carried out on the CCl4 induced rats by administration of aqueous extract of Bryophyllum pinnatum at doses of 50, 100 and 200mg/Kg bw for 14 consecutive days, and one group was treated with Silymarin at a dose of 50 mg/Kg as a standard drug. The antioxidant activities were estimated by evaluating its effects on antioxidant parameters in the tissues of albino rats (Serum, Liver and Heart). The activity of catalase (CAT), Superoxide dismutase (SOD), Glutathione peroxidase (GPx) and the levels of MDA and GSH were evaluated in tissue homogenates. Results showed that the rats fed with the higher concentrations of Bryophyllum pinnatum had significant increase (p<0.05) in glutathione content (GSH), GPx, SOD and catalase activities relative to normal and standard control. However, the level of MDA was decreased (p<0.05) for the treated groups (100 and 200mg/kg). These results suggest that aqueous extracts of Bryophyllum pinnatum has active substances contributing to varied degrees of potent antioxidant activity and could serve as important source of antioxidants in food, cosmetics and pharmaceutical industries.
Keywords: Oxidative injury, Bryophyllum pinnatum, antioxidant, Diseases, Tissues
Keywords:
Oxidative injury, Bryophyllum pinnatum, antioxidant, Diseases, TissuesDOI
https://doi.org/10.22270/jddt.v11i4.4877References
Birben EU, Murat C, Sackesen SE, & Kalayci O, "Oxidative Stress and antioxidant defense". World Allergy Organization Journal, 2012; 5:9-19. https://doi.org/10.1097/WOX.0b013e3182439613
LoboVA, Patil AP, & Chandra N, (2010). "Free radicals, antioxidants and functional foods: impact on human health", Pharmacognosy Reviews, 2010; 4:118-126. https://doi.org/10.4103/0973-7847.70902
Sisein EA, "Biochemistry of Free Radicals and Antioxidants" Sch Acad J Biosci, 2014; 2:110-118.
Jurkovič S, Osredkar J, Marc J, "Molecular impact of glutathione peroxidases in antioxidant processes" Biochemia Medica , 2008; 18 :162-174. https://doi.org/10.11613/BM.2008.016
Kurutas EB, "The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state" Nutrition Journal, 2016; 15:1-71. https://doi.org/10.1186/s12937-016-0186-5
Ramalakshmi K, RahathKI, Rao LJM, "Antioxidant potential of low-grade coffee beans" Journal of Food Science, 2007; 41:96-103 https://doi.org/10.1016/j.foodres.2007.10.003
Lans CA, "Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus" J. Ethnobiol. Ethnomed, 2006; 2:1-11. DOI: https://doi.org/10.1186/1746-4269-2-45
Bailey CJ and Day, "Traditional plant medicines as treatments for diabetes" Diabetes Care, 1989; 12: 553-564. https://doi.org/10.2337/diacare.12.8.553
Gornall AG, Bardawill CJ & David MM, "Determination of serum proteins by means of the biuret reaction" J. Biol. Chem., 1949; 177:751-66. https://doi.org/10.1016/S0021-9258(18)57021-6
Adam-vizi V, Seregi M, "Receptor dependent stimulatory effect of noradrenaline on Na+/K+ ATPase in rat brain homogenate: Role of lipid peroxidation" Biochem.Pharmacol., 1982; 31:2231-2236 https://doi.org/10.1016/0006-2952(82)90106-X
Ellman G, "Tissue sulfhydryl groups" Arch Biochem Biophys, 1959; 82: 70 -77. https://doi.org/10.1016/0003-9861(59)90090-6
Misra HP, Fridovich, I "The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase" Journal of Biological Chemistry, 1972; 247:3170-3175. https://doi.org/10.1016/S0021-9258(19)45228-9
Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972; 47:389-394. https://doi.org/10.1016/0003-2697(72)90132-7
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, and Hoekstra WG, "Selenium: Biochemical Role as a Component of Glutathione Peroxidase" Science, 1973; 179(4073):588-590. https://doi.org/10.1126/science.179.4073.588
Al-Jaber NA, Awaad AS, & Moses JE, "Review on some antioxidant plants growing in Arab world" Journal of Saudi Chemical Society. 2011; 15:293-307. https://doi.org/10.1016/j.jscs.2011.07.004
Magdi N, Ashour Dawoud F, Habib Rafaat A, Hanna Mahmoud A, El-Dabaa T, "Beneficial Effects of Curcumin and Ruta Chalepensis on the Antioxidant System and Inflammation in Hypercholesteromic Rats" Australian Journal of Basic and Applied Sciences , 2011; 5:2562-2567.
Manno M, Rezzadore M, Grossi M, Sbrana C, "Potentiation of occupational carbon tetrachloride toxicity by ethanol abuse" Hum Exp Toxicol., 1996; 15(4):294-300. PMID: 8845218. https://doi.org/10.1177/096032719601500404
Borrelli, GM & Trono D, "Molecular Approaches to Genetically Improve the Accumulation of Health-Promoting Secondary Metabolites in Staple Crops-A Case Study: The Lipoxygenase-B1 Genes and Regulation of the Carotenoid Content in Pasta Products" International Journal of Molecular Sciences, 2016; 17:1-33. https://doi.org/10.3390/ijms17071177
Hamid AA, Aiyelaagbe O O, "Antioxidants: Its medicinal and pharmacological applications" AJPAC 2010; 4(8):142-151.
Nwaneri-Chidozie VO, Idoko, VO, Salemcity AJ, "Assessment of Antioxidant Activity of Ethanol and n-Hexane Seed Extracts of Annona muricatain Rats" JJBS 2016; 9:4-15
Arivazhagan S, Balasenthil S, Nagini S, "Garlic and neem leaf extracts enhance hepatic glutathione and glutathione dependent enzymes during N-methyl- N nitrosoguanidine (MNNG)- induced gastric carcinogenesis" Phytotherapy Research, 2000; 1: 291-293. https://doi.org/10.1002/1099-1573(200006)14:4<291::AID-PTR570>3.0.CO;2-Y
Oyedemi SO, Bradley G, Afolayan AJ "In -vitro and In-vivo antioxidant activities of aqueous extract of Strychnos henningsii Gilg" AJPP, 2010; 4(2):70-78.
Oloyede GK, Onocha PA, Soyinka J, "Phytochemical screening, antimicrobial and antioxidant activities of four Nigerian medicinal plants" Ann Biol Res, 20101(2):114-120.
Sozmen EY, Sozmen B, Delen Y, Onat T,"Protective effect of Raphanus sativus extract against copper sulphate toxicity in Ctenopharyngodon idella fish" Arch Med Res, 2001; 32:283-287. https://doi.org/10.1016/S0188-4409(01)00285-5
Published
Abstract Display: 708 
PDF Downloads: 745 PDF Downloads: 212 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.