Evaluation of Phytocompounds and Chemical Elements Present In Selected Species of Seaweeds, to Sustain Future Quantitative Analysis for Bioactive Compounds
Abstract
Introduction: The specific physiological capacity of marine organisms, including seaweeds, to survive in extreme environmental conditions is associated to the production of secondary metabolites. Seaweeds are known as powerful source of a broad range of bioactive compounds. Objective: The aim of this study was to investigate the phytocompounds and chemical elements present in eight species of seaweeds which occur around Inhaca Island, Mozambique. Specifically, Halimeda cuneata, Pseudorhizoclonium africanum, Pseudocodium devriesii, Dictyota suhrii, Gracilaria salicornia, Hypnea rosea, Laurencia natalensis and Jania adhaerens. Methods: For phytocompounds and chemical elements screening, seaweeds samples were dried, ground to powder and extracted using methanol as solvent. The analyses were performed using GC-MS analysis, Energy Dispersive X-ray Fluorescence, and colorimetric protocols for phytocompound analysis. Results: A total of 82 phytocompounds were identified. Phytol and Z-8-Methyl-9-tetradecenoic were present in all samples analysed, while Cetene, 9-Octadecenoic acid (Z)-methyl ester, Desmoterol, Octadecanoic acid, and Oleic acid were the less common phytocompound identified. Campesterol, gama-Sitoesterol, Cholest-5-en-ol, 24-propylidene-(3.beta) are phytosterols only identified in green seaweeds. The concentration of chemical elements among the seaweeds species was different. However, Ca, Cl and K were presented in high concentration in some of the seaweeds analysed. Conclusion: Overall, the seaweeds analysed in this study, seems to be good candidate for further biotechnological application and deserve further investigation.
Keywords: seaweeds, methanolic extracts, phytocompounds, minerals, Inhaca Island.
Keywords:
seaweeds, methanolic extracts, phytocompounds, minerals, Inhaca IslandDOI
https://doi.org/10.22270/jddt.v10i5.4422References
Agatonovic-Kustrin S, Morton DW. Cosmeceuticals Derived from Bioactive Substances Found in Marine Algae. Oceanography, 2013; 1:106. doi:10.4172/2332-2632.1000106.
El-Din SM, El-Ahwany AMD. Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea). Journal of Taibah University for Science, 2016; 10:471-484.
Blunt JW, Copp BR, Hu WP, Munro MH, Northcote PT, Prinsep MR. Marine natural products. Natural Product Research, 2007; 24:31-86. doi:10.1039/b603047.
Pérez MJ, Falqué E, Domínguez H. Antimicrobial Action of Compounds from Marine Seaweed. Marine Drugs, 2016; 14, 52; 10.3390/md14030052.
Taskin E, Ozturk M, Taskin E, Kurt O. Antibacterial activities of some Marine algae from the Aegean sea (Turkey). African Journal of Biotechnology, 2007; 6:2746-2751.
Melpha Y, Manchu N, James JE. Phytochemical evaluation of two brown seaweeds from Muttom and Rasthacaud coasts of Tamil Nadu, India. Journal of Chemical and Pharmaceutical Research, 2014; 6:566-569.
Gullón B, Gagaoua M, Barba FJ, Gullón P, Zhang W, Lorenzo JM. Seaweeds as promising resource of bioactive compounds: Overview of novel extraction strategies and design of tailored meat products. Trends in Food Science & Technology, 2020; 100:1–18.
Cox S, Abu-Ghannam N, Gupta S. Effect of Processing Conditions On Phytochemical Constituents Of Edible Irish Seaweed Himanthalia Elongata. Journal Of Food Processing And Preservation, 2011; 36:348–363.
Marimuthu J, Antonisamy, Essakimuthu P, Narayanan J, Anantham B, Tharmaraj RJJM, Arumugam. Phytochemical characterization of brown seaweed Sargassum wightii. Asian Pacific Journal of Tropical Disease, 2012; 2:S109–S113.
Mansuya P, Aruna P, Sridhar S, Kumar J, Babu S. Antibacterial Activity and Qualitative Phytochemical Analysis of Selected Seaweeds from Gulf of Mannar Region. Journal of Experimental Sciences, 2010; 1:23-26.
Osman MEH, Aboshady AM, Elshobary ME. Production and characterization of antimicrobial active substance from some macroalgae collected from Abu- Qir bay (Alexandria) Egypt. African Journal of Biotechnology, 2013; 12:6847-6858.
Shanmugam J, Devi RK, Viswanathan S, Nallamuthu T. Antibacterial and antioxidant activity of red seaweeds from Kilakarai, Rameswaram, Tamilnadu, India. Journal of Pharmaceutical and Biomedical Sciences, 2013; 32:1386–1395.
Tanniou A, Vandanjon L, Incera M, Serrano-Leon E, Husa V. Assessment of the spatial variability of phenolic contents and associated bioactivities in the invasive alga Sargassum muticum sampled along its European range from Norway to Portugal. Journal of Applied Phycology, 2014; 2:1215–1230.
Bandeira SO. Seaweed resources of Mozambique. In: The Seaweed Resources of the World (Eds) Critchley AT; Ohno M, Japan International Cooperation Agency (JICA). 1998, 403–408.
De Clercket O, Bolton JJ, Anderson RJ, Coppejans E. Guide to the seaweeds of KwaZulu-Natal, Scripta Botanica Belgica. 2005, 33. i-283.
Coppejans E; Richmond MD; Clerck O; Rabesandratona R. Marine Macroalgae. Seaweeds. In: MD Richmond (ed) A Guide to the seashores of eastern Africa and the western Indian Ocean Islands. Sida. 1997, 70-97.
Critchley AT, Aken ME, Bandeira SO, Kalk M. A revised list of marine algae from Inhaca Island, Mozambique. South African Journal of Botany, 1994; 37:261–265.
Isaac WE. Some Marine Algae from Xai-Xai, South African Journal of Botany, 1957; 23:75-102.
Massingue AO, Bandeira SO. Distribution of Seagrasses and Common Seaweeds around Nampula Province (Northern Mozambique) with emphasis to Mozambique Island. Western Indian Ocean Journal of Marine Science, 2005; 4:175–183.
Bandeira SO, António CM, Critchley AT. A taxonomic listing, including new distribution records, for benthic, intertidal seaweeds from Mecúfi, northern Mozambique. South African Journal of Botany, 2001; 67:492-496.
Carvalho AM, Bandeira SO. Seaweed flora of Quirimbas Archipelago, northern Mozambique. In: Chapman ARO, Anderson RJ, Vreeland VJ and Davison IR (eds). Proceedings of the XVIIth International Seaweed Symposium, Cape Town, South Africa. 2003. Oxford University Press. ISBN 019 850742 9.
Critchley AT, Aken ME, Bandeira SO, Kalk M. A revised list of marine algae from Inhaca Island, Mozambique. South African Journal of Botany, 1997; 63:426-435.
Richmond MD. A guide to the Seashore of Eastern Africa and the Western Indian Ocean Islands. Wiomsa. 2010. p 464.
Abirami RG, Kowsalya S. Cosmeceuticals Derived from Bioactive Substances Found in Marine Algae. International Research Journal of Microbiology, 2012; 3:328-332.
Sitko R, Zawisza B. Quantification in X-Ray Fluorescence Spectrometry, X-Ray Spectroscopy. IntechOpen, 2012; doi: 10.5772/29367.
Noda T, Tsuda S, Mori M, Takigawa S, Matsuura-Endo C, Kim S. Determination of the phosphorus content in potato starch using an energy-dispersive X-ray fluorescence method. Food Chemistry, 2006; 95:632–637.
Deyab M, Elkatony T, Ward F. Qualitative and Quantitative Analysis of Phytochemical Studies on Brown Seaweed, Dictyota dichotoma. International Journal of Engineering Development and Research, 2016; 4:674-678.
Yogeswari S, Ramalakshmi S, Neelavathy R, Johnpaul M. Identification and Comparative Studies of Different Volatile Fractions from Monochaetia kansensis by GCMS. The Global Journal of Pharmacology, 2012; 6:65-71.
Garg K, Shrivastava B, Bhargava A. GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus. Journal of Drug Delivery and Therapeutics, 2019; 9:28-30.
Domettila C, Joselin J, Jeeva S. Phytochemical analysis on some south Indian seaweeds. Journal of Chemical and Pharmaceutical Research, 2013; 5: 275-278.
Nandagopalan V, Gritto MJ, Doss A. GC-MS analysis of bioactive components of the methanol extract of Hibiscus tiliaceus Linn. Asian Journal of Plant Science and Research, 2015; 5:6-10.
Aziz S, Jafarah N, Yusof B, Zetty Z. Phytol-containing seaweed extracts as control for Ganoderma boninense. Journal of oil palm research, 2019; 31: 238-247.
Kalsoom R, Haider MS, Chohan C. Phytochemical analysis and antifungal activity of some medicinal plants against Alternaria specie isolated from onion. Journal of Animal and Plant Sciences, 2020; 30:1–7.
Femi-Adepoju A, Fatoba PO, Adepoju A, Oluyori AP. Phytochemical analysis, antimicrobial activity and identification of phytoconstituents in Gleichenia pectinata (Willd.) C. Presl. International Journal of Biomedical and Advance Research, 2018; 9:400-406.
Egbung E, Anosike C, Utu-Baku A, Ogar I, Nna V. Phytochemical evaluation and GC-MS analysis of Hyptis verticillata cultivated in Calabar Cross River State, Nigeria. International Journal of Biological and Chemical Sciences, 2018; 11:2548-2559.
Kebelmann K, Hornung A, Karsten U, Griffiths G. Thermo-chemical behaviour and chemical product formation from Polar seaweeds during intermediate pyrolysis. Journal of Analytical and Applied Pyrolysis, 2013; 104:131-138.
Mou Y, Meng J, Fu X. Antimicrobial and antioxidant activities and effect of 1-hexadecene addition on palmarumycin C2 and C3 yields in liquid culture of endophytic fungus Berkleasmium sp. Dzf12. Molecules, 2013; 18:15587-15599.
Ogbe R. A Review of Dietary Phytosterols: Their occurences, metabolism and health benefits. Asian Journal of Plant Science and Research, 2015; 5:10 - 21.
Lopes G, Sousa C, Valentão P, Andrade PB. Sterols in Algae and Health. In Bioactive Compounds from Marine Foods (eds Hernández‐Ledesma B, Herrero M). 2013, DOI:10.1002/9781118412893.ch9.
Choi JM, Lee EO, Lee HJ. Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytotherapy Research, 2007; 21:954-959. doi:10.1002/ptr.2189.
Bakar K, Mohamad H, Latip J, Seng T; Herng M. Fatty acids compositions of Sargassum granuliferum and Dictyota dichotoma and their anti-fouling activities. Journal of Sustainability Science and Management, 2017; 12:8 – 16.
Pereira H, Barreira L, Figueiredo F. Polyunsaturated Fatty acids of marine macroalgae: potential for nutritional and pharmaceutical applications. Marine Drugs, 2012; 10:1920-1935.
Cowan MM. Plant Products as Antimicrobial Agents. Clinical Microbiology, 1999; 12:564-582.
Desai S, Desai DG, Kaur H. Saponins and their biological activities. Pharma Times, 2009; 41:13-16.
Okwu DE. Improving the Nutritive Value of Cassava Tapioca Meal with Local Spices. Journal of Nutraceuticals, Functional & Medical Foods, 2001; 3:43-50.
Jeeva S, Domettila C, Anantham B, Mahesh M. Preliminary phytochemical studies on some selected seaweeds from Gulf of Mannar, India. Asian Pacific Journal of Tropical Biomedicine, 2012; 2:S30–S33.
Ito K, Hori K. Seaweed: Chemical composition and potential food uses. Food Reviews International, 1989; 5:101-144.
Kim J, Pitts B, Stewart P, Camper A, Yoon J. Comparison of the Antimicrobial Effects of Chlorine, Silver Ion, and Tobramycin on Biofilm. Antimicrobial agents and chemotherapy, 2008; 52:1446-1453.
Published
Abstract Display: 970 
PDF Downloads: 978 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.