Comparative Qualitative Phytochemical Analysis of the Different Parts of Tinospora crispa: A Contribution to Sustainable Use of the Plant Species
Abstract
The plant screening for phytochemical constituents seems to have the potential to act as a source of useful drugs and cures many infections as a result of the presence of various bioactive compounds that evident to have enormous activity against array human pathogens. The objective of the study was to undertake a comparative qualitative phytochemical analysis of the different parts of Tinospora crispa (T. crispa), a traditional herb used against several diseases. The different parts of T. crispa were extracted using maceration method. The results of phytochemical screening indicated that T. crispa contains alkaloids, flavonoids, tannins, saponins, steroids and terpenoids. The TLC profiles of samples, depicted through the Rf values of resolved compound bands and the solvent system selected for the best results of TLC was Toluene: Ethyl acetate: Formic acid (7:5:1) and (5:4:1) for gallic acid and quercetin respectively. The study will provide referential information for the correct identification of the bioactive compounds and a suitable solvent system for separation of those compounds from the T. crispa. These findings suggested that T. crispa leaves and flower extract could be a potential source of drugs which in future may serve for the production of synthetically improved therapeutic agents.
Keywords: Tinospora crispa, Phytochemical screening, TLC profiles, Gallic acid, Quercetin
References
Akroum S, Satta D, Lalaoui K. Eur. J. Sci. Res, 2009; 2:289-295
Sato OY, Singyouchi H, Ohtsubo K, Kihara T, Shibata HM, International Journal of PharmaceuticalSciences and Research, 1997, 20:401-403
Joy PP, Thomas J, Samuel M, Baby PS, Aromatic and medicinal plants. Kerela agricultural university india, 1998, Pp 44-46.
Mongole AJ, Awati R, Chaturvedi A, Zanwar P, International Journal of Pharm Tech Research, 2010, 2: 2307-2312
Sneden AT, Natural Products as Medicinally Useful Agents, 2004, Pp21-23
Srivastava B, Sharma VC, Pant P, Pandey N, Jadhav A. Evaluation for substitution of stem bark with small branches of Myrica esculenta for medicinal use–A comparative phytochemical study. J Ayurv Integrat Med 2016; 7(4):218-223.
Senguttuvan J, Paulsamy S, Karthika K. Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific J Trop Biomed 2014; 4: S359-S367.
Sulaiman M.R., Zakaria Z.A., Lihan R., 2008. Antinociceptive and anti-inflammatory activities of Tinospora crispa in various animal models. Int. J. Trop. Med. 3 (3), 66–69.
Dweck A.C., Calvin J.P., Andawali (Tinospora crispa) – a review. Pers. Care Mag. 2006; 7(1):1–7.
Najib N.A.R., Furuta T., Kojima S., Takane K., Ali M.M. Antimalarial activity of extracts of Malaysian medicinal plants. J. Ethnopharmacol. 1999; 64:249–254.
Zakaria Z.A., Mat Jais A.M., Somchit M.N., Sulaiman M.R., Faizal F.O., The in vitro antibacterial activity of Tinospora crispa extracts. J. Biol. Sci. 2006; 6(2):398–401.
Kongkathip N., Dhumma-upakorn P., Kongkathip B., Chawananoraset, K., Sangchomkaeo, P., Hatthakitpanichakul, S., Study on cardiac contractility of cycloeucalenol and cyloeucalenone isolated from Tinospora crispa. J. Ethnopharmacol. 2002; 83:95–99.
Noor H., Ashcroft S.J.H., Antidiabetic effects of Tinospora crispa in rats. J. Ethnopharmacol. 1989; 27:149–161.
Noor H., Ashcroft S.J.H., Pharmacological characterisation of the antihyperglycaemic properties of Tinospora crispa extract. J. Ethnopharmacol. 1998; 62:7–13.
Umi Kalsom Y., Noor H., Flavone O-glycosides from Tinospora crispa. Fitoterapia 1995; 66(3):280
Bisset B.C., Nwaiwu M.K., Quaternary alkaloids of Tinospora spp.. Planta Med. 1984; 48(4):275–279.
Misak N.Z., Adsorption isotherms in ion exchange reactions. Further treatments and remarks on the application of the Langmuir isotherm. Colloid. Surf. A: Physicochem. Eng. Asp. 1995; 97:129–140.
Kongkathip, N., Dhumma-upakorn, P., Kongkathip, B., Chawananoraset, K., Sangchomkaeo, P., Hatthakitpanichakul, S., Study on cardiac contractility of cycloeucalenol and cyloeucalenone isolated from Tinospora crispa. J. Ethnopharmacol. 2002; 83:95–99.
Mukherjee, P. K., “Quality Control of Herbal Drugs”, 2nd Edition, Business Horizons, 2007, 2-14.
Khandelwal KR, Practical pharmacognosy technique and experiments. 23rd Ed. Nirali Prakashan; 2005.
Kokate CK. Practical pharmacognosy. 4th Ed. Vallabh Prakashan; 1994.
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