Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur

  • Soulaf MEHLOUS Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Fatima BENCHIKH Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Hassiba BENABDALLAH Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Karima LOUCIF Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria
  • Chahrazed KAOUDOUNE Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria
  • Hocine LAOUER Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria
  • Smain AMIRA Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria
DOI https://doi.org/10.22270/jddt.v10i4.4166

Abstract

This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants.


Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.

Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids

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Author Biographies

Soulaf MEHLOUS, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Fatima BENCHIKH, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Hassiba BENABDALLAH, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Karima LOUCIF, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Animal Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif-1, 19000, Algeria

Chahrazed KAOUDOUNE, Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria

Laboratory of Environmental Biosurveillance, Faculty of Sciences, Department of Biology, BadjiMokhtar University, 23000-Annaba, Algeria

Hocine LAOUER, Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Smain AMIRA, Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

Laboratory of Valorisation of Natural Biological Resources, Department of Biology, University of Setif1, Algeria

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MEHLOUS S, BENCHIKH F, BENABDALLAH H, LOUCIF K, KAOUDOUNE C, LAOUER H, AMIRA S. Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur. JDDT [Internet]. 15Jul.2020 [cited 5May2024];10(4):188-90. Available from: https://www.jddtonline.info/index.php/jddt/article/view/4166
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Vol 10 No 4 (2020): Volume 10, Issue 4, July-August 2020
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Research
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