Anti-Inflammatulatory Activity of Hydro-Ethanolic Bark Extracts of Piliostigma reticulatum Hochst (Caesalpiniaceae) and Analysis by HPLC-DAD and HPLC-MS of its Methanol Fraction
Abstract
Use of plants as remedies dates back to the time of the oldest civilizations. Among these active ingredients, polyphenols play an important role. Piliostigma reticulatum, a plant whose barks are rich in condensed tannin is often used as an anti-inflammatory. The purpose of this work was to correlate the anti-inflammatory activity of extracts with their chemical composition through chromatography fractionation analyses (HPLC-DAD and HPLC-MS). The barks were extracted by moderate hydroethanol decoction followed by silica gel splitting with successively ethyl acetate, methanol and water. Anti-inflammatory activity was evaluated by the method of carrageenan induced hind paw edema in Wistar rats. The chemical study was carried out by HPLC-DAD and HPLC-MS after isolation of the compounds by fractionation on Sephadex LH-20 gel. The bark hydroethanolic extract had shown ability to inhibit significantly the inflammation induced by carraghenan. Among the hydroethanolic bark extract fractions that methanol fraction had the best activity at 10 mg/kg with inhibition percentages similar to those of acetylsalicylic acid with 48.51% ± 2.26; 54.71% ± 5.13; 64.19% ± 6.70 vs. 54.69% ± 7.23; 57.83% ± 9; 65.13% ± 8.44 respectively. The HPLC-DAD analysis showed that the SF5 sub-fraction presented the best chromatogram with several peaks, three of which were high intensity. The latter would correspond to monomer, dimer and trimer of catechin according to the weights [M-H] + m / z: 291.07; 579.18; 867.27 obtained by HPLC-MS. The compounds responsible for anti-inflammatory activity would be condensed tannins. The latter would consist mainly of catechin oligomers.
Keys words: Piliostigma reticulatum, bark, anti-inflammatory activity, HPLC-DAD, HPLC-MS
Keywords:
Piliostigma reticulatum, anti-inflammatory activity, HPLC-MSDOI
https://doi.org/10.22270/jddt.v10i3.4058References
Maisuthisakul P, « Phenolic Constituents and Antioxidant Properties of some Thai Plants », in Phytochemicals - A Global Perspective of Their Role in Nutrition and Health, V. Rao, Éd. InTech, 2012.
Wargovich M. J. Anticancer properties of fruits and vegetables. HortScience. 2000; 35 (4):573–575.
Olusola A, Akomolafe S. F and Abayomi T. G. Antioxidant Potential of the Leaf Extract of Pilostigma Thonningii (Caesalpiniacea). J. Atoms and Molecules, 2013; 3(2):474–477
Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajasb F, P.C. Tripathic, Seala T, Mukherjee B. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. Journal of Ethnopharmacology, 2003; 84(2‑3):131‑138
Latté K. P. et Kolodziej H. Antioxidant Properties of Phenolic Compounds from Pelargonium reniforme. Journal of Agricultural and Food Chemistry. 2004; 52 (15):4899‑4902.
Tabart J, Kevers C, Pincemail J, Defraigne J.O et Dommes J. Comparative antioxidant capacities of phenolic compounds measured by various tests. Food Chemistry. 2009 ; 113(4):1226‑1233.
Dieng S I M, Mathieu C., Sarr A. Diatta-Badji K. et Fall A D. Condensed Tannins Content and their Influence on the Antioxidant Activity of Bark Hydroethanol Extract of Piliostigma reticulatum (Dc) Hochst and its Fractions. Pharmacognosy Journal. 2020; 12(2):361‑368.
Aderogba A. M., Okoh E. K., Okeke I. N.et Olajide M. Anti-microbial and anti-inflammatory effects of Pilostigma reticulatum leaf extract. International journal of pharmacology. 2006; 2(1):70 -74.
Yelemou B, Bationo B. A, Yameogo G, et Rasolodimby J. M. Gestion traditionnelle et usages de Piliostigma reticulatum sur le Plateau central du Burkina Faso. BOIS & FORETS DES TROPIQUES. 2007; 291(291):55‑66.
Arbonnier M., Arbres, arbustes et lianes des zones sèches d’Afrique de l’Ouest. Editions Quae; 2009.
Labourel G. et Péaud-Lenoel C. Separation par Chromatographie sur colonne de silice des glucofructosanes de la série inuline de D. P. entre 1 et 20.. Chem. zvesti ,1969; 23:765-769
Yang C., Li D. et Wan X. Combination of HSCCC and Sephadex LH-20 methods: An approach to isolation and purification of the main individual theaflavins from black tea. Journal of Chromatography B. 2008; 861(1):140‑144.
Winter C. A, Risley E. A et Nuss G. W. Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs. Proceedings of the Society for Experimental Biology and Medicine. 1962; 111(3):544‑547.
Sene M. Barboza F S, Sarr A, Outouen D T, Wele A, Bassene E and Sy G Y. Analgesic and anti-inflammatory activity of methanolic fraction of total ethereal leaf extract of Annona senegalensis Pers. (Annonaceae). Afr. J. Pharm. Pharmacol. 2017; 11(8):120‑124.
Nariman F, Eftekhar F, Habibi Z. et Falsafi T. Anti-Helicobacter pylori Activities of Six Iranian Plants. Helicobacter. 2004; 9 (2):146‑151.
Ferreira D. et Slade D. Oligomeric proanthocyanidins: naturally occurring O-heterocycles. Natural Product Reports. 2002; 19 (5):517‑541.
Bruneton J. Pharmacognosie, phytochimie, plantes médicinales (4e éd.), Tec et Doc. Paris, 2009.
Macáková K, Kolečkář2 V, Cahlíková L, Chlebek J, Hošťálková A, Kamil K, Daniel J and Lubomír Opletal. Tannins and their Influence on Health », in Recent Advances in Medicinal Chemistry. 2014; 159‑208.
Kantz K. et Singleton V. L. Isolation and Determination of Polymeric Polyphenols Using Sephadex LH-20 and Analysis of Grape Tissue Extracts. Am J Enol Vitic. 1990; 41(3):223‑228.
Chen L.G. et Wang C.C. Preparative separation of oligostilbenes from Vitis thunbergii var. taiwaniana by centrifugal partition chromatography followed by Sephadex LH-20 column chromatography. Separation and Purification Technology. 2008; 66(1):65‑70.
Kim S. M, Kang S. W, Jeon J. S, et Um B. H. A comparison of Pycnogenol® and bark extracts from Pinus thunbergii and Pinus densiflora: Extractability, antioxidant activity and proanthocyanidin composition. 2012; 6(14):2839-2849.
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