Development of liposome encapsulated curcumin for treatment of arthritis

Abstract

Liposomesof curcumin was prepared by Thin Film Hydration method (TFH) and Reverse Phase Evaporation Method (REV) methods and Freeze-thaw (F-T) cycles were carried out for liposomes prepared by both the methods, then optimized with regard to percentage drug entrapment by changing various process and formulation parameters, various compositions of optimized liposomal batches along with their PDE and mean vesicle size values are recorded. Prepared liposomes prior to size reduction were suitable for nasal delivery as then vesicle size distribution was in range of 10-20 pm that is required for nasal drug delivery. and finally optimized final batch parameter were evaluated and conclude ration of lipid and drug were-Drug: PC :CHOL (1:4:1), REV method followed by F-T cycles and Diethyl ether :water (5:1) solvent systems were used and  PDE/D[4,3] before size reduction was 97.89±0 31/ 12.8±0.2 and PDE/D[4,3] after size reduction 98.3 ± 0.21/2.5 ± 0.01 were obtained. However, further processing for size reduction of liposomes was required for lung deposition in case of pulmonary drug deliver) and hence liposomes after size reduction to 3-5 pm were used for preparation of DPI formulations.

Keywords: Liposomes, Thin Film Hydration, Reverse Phase Evaporation and PDE