A PRECISE RP-HPLC METHOD DEVELOPMENT FOR THE SIMULTANEOUS ESTIMATION OF DIAZEPAM AND PROPRANOLOL HYDROCHLORIDE IN TABLET DOSAGE FORM

Abstract

A simple, precise, and accurate RP – HPLC method was developed and validated for the simultaneous estimation of Diazepam and Propranolol Hydrochloride in bulk and tablet dosage form. Isocratic elution at a flow rate of 1.0 mL/min was employed on BDS Hypersil C18 (250 X 4.6 I.D., 5µm particle size) at ambient temperature. The mobile phase consisted of mixed buffer (0.02M potassium dihydrogen ortho phosphate and 0.003M dipotassium hydrogen phosphate, pH adjusted to 3.0) and acetonitrile (40:60v/v). The UV detection wavelength was 222nm and 20µL of sample was injected. The retention times of Diazepam and Propranolol Hydrochloride were 2.031min and 5.597min respectively. The linearity was obtained in the range of 2 – 12 µg/mL for diazepam and 16 – 96 µg/mL for propranolol hydrochloride. The mean % recovery of diazepam and propranolol hydrochloride was found to be 99.92, 99.94 respectively. The % RSD for precision and accuracy of the method was found to be less than 1%. The method was validated as per the ICH guidelines. The method developed was found to be precise and accurate for the simultaneous estimation of diazepam and propranolol hydrochloride in tablet dosage forms