To perform phytochemical screening and study the antioxidant potential of isolated compound from Hemidesmus indicus
Abstract
“Anantmul” is an important and widely used medicinal plant. The study aimed to determine the physicochemical composition, bioactive compounds and antioxidant activity of Hemidesmus indicus (Asclepiadaceae). The roots of the plant were collected and sequentially extracted using petroleum ether, ethyl acetate, and methanol. The preliminary phytochemical screening of extracts was carried out and found to be a good source of bioactive compounds. Biological activities of flavonoid and phenolic compounds have been discovered in several latest studies. Further phytochemical isolation was carried out, and Lupeol was isolated. Phenols are shown to be multifunctional antioxidants which will perform as singlet oxygen quenchers. Lupeol was evaluated for in-vitro antioxidant activity. It showed a correlation with antioxidant activity by DPPH (IC50 = 0.52, P 6 0.05) and H2O2 (IC50 = 0.43, P 6 0.05). The results show promising perspectives for the exploitation and use of anantmul rhizome as a constituent of anti-aging as well as anticancer diet.
Keywords: Anantmul, Lupeol, Antioxidant, DPPH
DOI
https://doi.org/10.22270/jddt.v9i2.2401References
Predes FS, Ruiz AL, Carvalho JE, Foglio MA, Dolder H. Antioxidant and in vitro antiproliferative activity of Arctium lappa root extracts. BMC Complem Altern Med. 2011; 11(25).
Gupta NS. The Ayurvedic System of Indian Medicine, New Delhi, Bharatiya Kala Prakashan. 2006; I:96-97
Sethi A, Srivastav SS, Srivastav S. Pregnane glycoside from Hemidesmus indicus. Indian J Heterocycl Chem 2006; 16:191-192.
Pandey KK, Dwivedi M, Urinary Tract Infection and its Management by Renalka. The Antiseptic. 2001; 98:295- 296.
Kumar S, Pooja M, Harika K, Haswitha E, Nagabhushanamma G, Vidyavathi N. In vitro Antioxidant Activities, Total Phenolics and Flavonoid Contents of whole plant of Hemidesmus indicus, Asian Journal of Pharmaceutical and Clinical Research. 2013; 6(2):249-251.
Kurupati VK, Nishteswar, K Phytochemical and clinical evaluation of Sariba (Hemidesmus indicus) on wound healing. International Research Journal of Pharmacy. 2012; 3(3):277-281.
Subramanian S, Abarna A, Thamizhiniyan V. Antihyperglycemic, Antioxidant and Antidyslipidemic properties Hemidesmus indicus R. Br. root extract studied in Alloxan –Induced Experimental Diabetes in Rats; IJPSR. 2012; 3(1):227-234
Mehta A, Sethiya NK, Mehta C, Shah GB. Anti-arthritis activity of roots of Hemidesmus indicus R.Br. (Anantmul) in rats, Asian Pacific Journal of Tropical Medicine. 2012, 130-135
Fimognari C, Lenzi M, Ferruzzi L, Turrini E, Scartezzini P et al., Mitochondrial Pathway Mediates the Antileukemic Effects of Hemidesmus indicus, a Promising Botanical Drug. Plos one. 2011; 6(6):e21544.
Nutan, Kumar, N , Saxena, Cytotoxic effect of Hemidesmus indicus R. Br. on HCT 116 human colon cell lines. 2019; 8(1):86-89.
Harborne JB. Phytochemical methods, London. Chapman and Hall, Ltd, 1973, 49-188.
Stahl E., Thin Layer Chromatography: A Laboratory Handbook, Second Edition, Springer, 1969, 206-258.
Chatterjee, I, Chakravarty , AK, Gomes, A. Daboia russellii and Naja kaouthia venom neutralization by lupeol acetate isolated from the root extract of Indian sarsaparilla Hemidesmus indicus R.Br. Journal of Ethnopharmacology 2006; 106:38–43.
Silva BM, Andrade PB, Valentão P, Ferreres F, Seabra RM, Ferreira MA. Quince, (Cydonia oblonga Miller) fruit (Pulp, Peel and Seed) and jam: Antioxidant activity. J Agr. Food Chem. 2004, 52:4705-4712.
Yang GJ, Yuan J. In vitro antioxidant properties of rutin, Lebensm. Wiss Technol. 2008; 41:1060-1066.
Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15:7313-52..
Jain, P. S. and Bari, S. B., ‘Isolation of Lupeol, Stigmasterol and Campesterol from Petroleum Ether Extract of Woody Stem-Bark of Wightia tinctoria’, Asian Journal of Plant Sciences, 2010; 9(3):163- 167
Paul , S., Hossen, M. S. , Tanvir, E. et al., “Antioxidant properties of Citrus macroptera fruit and its in vivo effects on the liver, kidney and pancreas in wistar rats,” International Journal of Pharmacology, 2015; 11(8):899–909.
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