Acute and sub-acute toxicity studies of flower extract of Tridex procumbens in albino rats

Abstract

Aim- The study was designed to evaluate the Acute & Sub-acute Toxicity Studies of Flower Extract of Tridex procumbens. Material & Methods-Dried powder of flower part was extracted by using chloroform: methanol solvent by using soxhlet apparatus for 72 hrs. Preliminary phytochemical screening was performed for the presence of various phytoconstituents. The chloroform: methanol extract was evaluated by using the acute and sub-acute toxicity studies. The acute toxicity study was also evaluated by administration of extract after oral and intraperitoneal extract. Various parameters i.e. body weight, clinical signs, hematological parameters and clinical chemistry parameters were evaluated. Result- In acute toxicity studies, there were no side effects at the dose of 2000 mg/kg. The sub-acute toxicity was performed at 100, 300 and 1000 mg/kg. There were some changes in body weight, organ weights, hematological parameters and clinical chemistry parameters. However, no toxic effects were seen at his dose. Conclusion- The results showed that no death of experimental rats at an oral dose of 2000 mg/kg body weight symptomatic of that the chloroform: methanol extract of Tridex procumbens flower is virtually non-toxic after oral dose exposure presuming that its components were engrossed. The extract can then be concluded to be safe for oral use as a traditional herbal remedy for the different ailments.

Keywords: Flower extract, acute toxicity, sub-acute study, chloroform: methanol extract, Tridex procumbens