Development and validation of stability indicating UPLC method for the estimation of ticagrelor in bulk and its tablet dosage form

  • J Omaima Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India
  • , Shyamala Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India
  • J V C Sharma Principal, Joginpally B R Pharmacy College, Hyderabad, T.S., India
DOI https://doi.org/10.22270/jddt.v9i1-s.2305

Abstract

The objective of the method was to develop a simple, rapid, sensitive, precise, accurate and validated Ultra Performance Liquid Chromatographic (UPLC) method for the estimation of Ticagrelor in tablet dosage form. Chromatographic separation was achieved on an acquity UPLC BDS C8 (150 x 4.6 mm, 5m) column with a mobile phase composed of Buffer 0.1% OPA (2.2 pH) and  Acetonitrile  in the ratio of 60:40   at a flow rate of 1.0 ml/min and 1 μl injection volume. The effluents were detected at a wavelength of 240 nm using TUV detector. The retention time of Ticagrelor was  found to be at 0.942 min. %RSD of the Ticagrelor was found to be 0.7  The method was validated with respect to specificity, accuracy, linearity, precision, robustness. The correlation coefficient for Ticagrelor was found to be 0.999. Recovery of Ticagrelor in formulation was found to be 99.51% . LOD, LOQ values obtained from regression equations of Ticagrelor were 0.45, 1.35 respectively.  Due to simplicity, high precision and rapidness the method can be successfully applied for   estimation of Ticagrelor in tablet dosage form.


Keywords: Ultra Performance Liquid Chromatographic, Ticagrelor, Tablet dosage form.

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Author Biographies

J Omaima, Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India

Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India

, Shyamala, Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India

Department of Pharmaceutical Analysis and Quality Assurance, Joginpally B R Pharmacy College, Hyderabad, T.S., India

J V C Sharma, Principal, Joginpally B R Pharmacy College, Hyderabad, T.S., India

Principal, Joginpally B R Pharmacy College, Hyderabad, T.S., India

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1.
Omaima J, Shyamala , Sharma JVC. Development and validation of stability indicating UPLC method for the estimation of ticagrelor in bulk and its tablet dosage form. JDDT [Internet]. 15Feb.2019 [cited 17Apr.2024];9(1-s):201-5. Available from: https://www.jddtonline.info/index.php/jddt/article/view/2305
Issue
Vol 9 No 1-s (2019): Volume 9, Issue 1-s Jan-Feb 2019
Section
Research
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