EVALUATION OF HYPOGLYCAEMIC, HYPOLIPIDAEMIC AND NON TOXIC EFFECT OF HYDRO-METHANOLIC EXTRACTS OF ZIZIPHUS MAURITIANA, ZIZIPHUS SPINA CHRISTI FRUIT AND GLIBENCLAMIDE ON ALLOXAN INDUCED DIABETIC RATS
Abstract
The use of plant products in the management of diabetes has gained ground in pharmacotherapy. It becomes imperative to evaluate the antidiabetic effects of fruit extracts of Ziziphus mauritiana (HMZM), Ziziphus spina christi (HMZS) and Glibenclamide on blood glucose, total protein, albumin and lipid profile in alloxan induced diabetic rats. 68 albino rats weighing 70 - 130g were used in this study. 26 rats were used for acute toxicities of Ziziphus mauritiana and Ziziphus spina christi. 42 rats of 7 groups of 6 rats each were used to test antidiabetic effects of Ziziphus mauritiana and Ziziphus spina christi plant extracts. Group 1 served as the negative control, groups 2-7 were intraperitoneally administered 360mg/kg of alloxan in normal saline respectively. Group 2 served as positive control, groups 3 and 4; 5 and 6 were respectively administered daily dose of 200 and 400mg/kg of HMZM and HMZS, group 7 was administered 0.21mg/kg of glibenclamide. Results showed Ziziphus mauritiana and Ziziphus spina christi non toxic at dose of 5000mg/kg. 48 hours after alloxan administration, blood glucose levels were found to be significantly higher (P<0.05) in groups 2-7 compared to group 1, thus confirming induction of diabetes. In groups 3-7, on 7th and 14th days of administration of extracts and Glibenclamide, there was a significantly lower (p<0.05) serum glucose, triacylglycerol, High density Lipoprotein, Low density lipoprotein and significantly higher (P > 0.05) serum level of total protein and albumin compared to group 2. The results obtained showed a significantly lower (P < 0.05) serum level of glucose. The effects of HMZMand HMZS fruit extracts on blood glucose, total protein, albumin and lipid profile was dose dependent. Conclusively, this study has demonstrated the antidiabetic effects of HMZM and HMZS with HMZS having a more pronounced effect on Blood glucose and lipid profile.
Keywords: Antidiabetic, Blood glucose, lipid profile, Glibenclamide, Ziziphus mauritiana, Ziziphus spina Christi.
DOI
https://doi.org/10.22270/jddt.v8i3.1711References
Ann Pietrangelo and Peggy Pletcher (2016). What Are the Different Types of Diabetes? Healthline News Letters. Retrieved 01/04/2018.
Cooke DW, Plotnick L. Type 1 diabetes mellitus in pediatrics. Pediatr Rev. 2008; 29(11):374–84.
Shoback D. Greenspan's basic & clinical endocrinology (9th Ed.). New York: McGraw-Hill Medical, edited by David G. Gardner. ISBN 0-07-162243-8. 2011; 342-350.
Go A. S. et al. Heart disease and stroke statistics–2013 update: a report from the American Heart Association. Circulation 127, e6–e245, doi: 10.1161/CIR.0b013e31828124ad (2013).
Fredrick L. Dunn (1990). Hyperlipidemia in diabetes mellitus. Wiley online library; Diabetes Metabolism Research and Review. Volume 6 Issue 1. Pages 47 – 61.
Clinical Practice Guidelines Expert Committee (CPGEC) (2013). Etiologic Classification of Diabetes Mellitus, 2013 Clinical Practice Guidelines for the Prevention and Management of Diabetes in Canada (Canadian Journal of Diabetes, vol. 37, p. S197), Canadian Diabetes Association.
Leslie D. and Valeri C. (2003) “LADA (Latent Autoimmune Diabetes in Adults)â€, Diabetes Voice, Vol. 48, p.14-16, International Diabetes Federation.
Manash P. Borgohain, Mangala Lahkar, Sahabuddin Ahmed, Liakat Chowdhury, Saurabh Kumar, Rajat Pant and Abhinav Choubey, Small Molecule Inhibiting Nuclear Factorâ€kB Ameliorates Oxidative Stress and Suppresses Renal Inflammation in Early Stage of Alloxanâ€Induced Diabetic Nephropathy in Rat, Basic & Clinical Pharmacology & Toxicology, 2017; 120(5):(442-449.
Ira J. Goldberg. Diabetic Dyslipidemia: Causes and Consequences. The Journal of Clinical Endocrinology & Metabolism, 2001; 86(3): 965–971.
Alssema M, Dekker JM, Kuivenhoven JA, Nijpels G, Teerlink T, Scheffer PG, Diamant M, C. Stehouwer CDA, Bouter LM,Heine RJ, Elevated cholesteryl ester transfer protein concentration is associated with an increased risk for cardiovascular disease in women, but not in men, with Type 2 diabetes: the Hoorn Study, Diabetic Medicine, 2007; 24(2):117-123.
Ginsberg HN. Diabetic dyslipidemia: basic mechanisms underlying the common hypertriglyceridemia and low HDL cholesterol levels. Diabetes. 1996; 45(Suppl 3):S27–S30.
Bai D. et al. Hyperglycemia and hyperlipidemia blunts the Insulin-Inpp5f negative feedback loop in the diabetic heart. Sci. Rep. 2016; 6, 22068; doi: 10.1038/srep22068.
Idoko A., Ikpe V. P. O, Rita O. N, Nelson N. O, Effiong JU, Alhassan A. J, Muhammad I. U, Abubakar N, Abubakar S. M Ugwudike P.O. Hypoglycemic and lipid lowering effect of aqueous fresh Leaf extract of Chromolaena odorata (linn) in albino wistar rats fed different concentrations of cholesterol enriched diet. Universal Journal of Pharmaceutical Research. 2018; 3(1):37-42.
Bhargava A and Rana A.C. Anti diabetic activity of Ziziphus mauritiana Lam. In streptozotocin induced Diabetic Rats and its comparison with some standard flavonoids. International Journal of Pharmaceutical Erudition 2014; 4(1):10-15.
Adzu, B., Amos, S., Amizan, M.B. and Gamaniel, K. Evaluation of the antidiarrhoeal effects of Ziziphus spina christi stem bark in rats. Acta. Trop. 2003; 87(2):245-250.
Hala M.H., Eman M.E., Aataa A.S. Antihyperglycemic,antihyperlipidemic and antioxidant effects of Ziziphus spina christi and Ziziphus jujube in alloxan induced diabetic rats. International Journal of Pharmacology 2006; 2(5):563-570.
Eurich M., Blackburn F.A., Majumdar D.F., Tsuyuki S.R., Varney R.T., Johnson J.A. "Benefits and harms of antidiabetic agents in patients with diabetes and heart failure: systematic review". BMJ (Clinical research ed.) 2007; 335(7618): p 497.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 2001; 50:536–546.
Lorke D. A new approach to practical acute toxicity testing. Arch. Toxicol. 1983; 54: 275-287.
Barham D and Trinder P. Colorimetric method for the determination of serum glucose. Clinical Pathology Analyst. 1972; 97:42.
Tietz N.W. (1995). Clinical Guide to Laboratory Tests, 3rd edition. WB Saunders Cmpany. Philadephia. PA Pp. 518-519.
Trinder P. Cholesterol enzymatic end point manual. Ann. Clin. Biochem. 1969; 6:24-27.
Friedewald W.T., Levy R.I, Fredrickson D.S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem; 1972; 18:499-502.
Graph Pad Instat3 Software; 2000. Available:www.graphpad.com (Graph Pad Instat3, 2000)
Hodge A. and Sterner B. (2005) Toxicity Classes. In: Canadian Center for Occupational Health and Safety. Retrieved from http://www.ccohs.ca/oshanswers/chemicals/id50.htm.
Jahodar L. Plants with hypoglycemic effects. Ceskoslovenska Farmacie, 1993; 42(6):251-259.
Lenzen S. The Mechanisms of Alloxan- and Streptozotocin-induced Diabetes. Diabetologia, 2008; 51(2):216–226.
Rabia N., Mir A.K., Kiran Y.K., Mushtaq A., Paras M., Barkat A., Mazhar M., and Muhammad, Z. A Review on Ziziphus as Antidiabetic. Journal of Applied Pharmaceutical Science; 2012; 2(3):177-179.
Baynes J.W. Role of oxidative stress in development of complications in diabetes. Diabetes 1991; 40:405-412.
Mohamadin A.M., Mariee A.D., El-Hefnawy H.M., and Fath E.M. Hypoglycemic activity of green tea extract in streptozotocin induced diabetic rats. Arab Journal of Laboratory Medicine, 2003; 29:397-400.
Tessier D. Glibenclamide vs gliclazide in type 2 diabetes of the elderly. Diabet Med. 1994; 11(10):974-980.
Sharma S.B., Hasir A., Prabhu K.H., Murthy, P.S., and Dev, G. Hypoglycemic and hypolipidemic effects of ethanolic extract of seeds of Eugenia jambolona in alloxan-induced diabetic rabbits. J. Ethnopharmacol. 2003; 85:201-206.
Al-Shamaony L., Al-Khaznaji S.M and Tway H.A.A. Hypoglycaemic effect of Artemisia herba alba II: Effect of valuable extract on some blood parameters, in diabetic animals. J. Ethnopharmacol. 194; 43:167-171.
Idoko A, Ikpe VPO, Nelson NO, Effiong JU, Alhassan AJ, Muhammad IU, Abubakar N, Abubakar SM. Effects of Lime Juice and Honey on Lipid Profile of Cholesterol Enriched Diet Fed Rat Model. Annual Research & Review in Biology; 2017; 20(3):1-10.
Zhao H.L., Sim J.S., Shim S.H and Ha Y.W. Antiobese and Hyperlipidaemic effects of platycodin saponins in diet induced obese rats. Evidences for lipase inhibition and calorie intake restriction. Int. J. Obese, 2005; 29:983-990.
Yugarami, T., Tan, B.K.H., The, M and Das, N.P. Effects of polyphenolic natural Products on the lipid profile of rats fed high fat diets. Lipids, 1992; 27:181-186.
Cisse A., Ndiaye A., Lopez-Sall P., Seck F. and Faye B. Antidiabetic Activity of Ziziphusmauritiana. Dakar Medical, 2000; (45):105-107.
Felig P., Wahren J., Sherwin, R and Palaiologos G. Amino acid and protein metabolism in diabetes mellitus. Arch. Intern. Med. 1977; 137:507-513.
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