ANTIDIABETIC ACTIVITY OF CALOTROPIS GIGANTEA WHITE FLOWER EXTRACTS IN ALLOXAN INDUCED DIABETIC RATS
Abstract
Calotropis gigantea white (Asclepiadaceae) has been documented as a traditional treatment of diabetes. The aim of this study was to evaluate the antidiabetic activity of chloroform and ethyl acetate extracts from the flower in normal and alloxan-induced diabetic rats. Blood samples were obtained and blood glucose levels were analyzed employing a glucometer at different time intervals on 1st, 4th, 8th and 12th day of the treatment with C. gigantea white extracts compare to glibenclamide (10 mg / kg). From the results, it is revealed that the test chloroform, and ethyl acetate extracts at a dose level 500 mg / kg, showed a significant reduction in blood sugar level day in a progressive manner comparable to glibenclamide. Biochemical parameters, including hemoglobin, urea, creatinine, serum cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were also assessed. Treatment of diabetic rats with the two extracts of this plant restored the elevated biochemical parameters significantly (p<0.05) to the normal level. It also proves the traditional claim with regard to C. gigantea white for its anti-diabetic activity.
Keywords: Calotropis gigantea white flowers, antidiabetic activity, alloxan, lipid profile.
Keywords:
Drug Delivery, Therapeutics, phytomedicineDOI
https://doi.org/10.22270/jddt.v7i3.1447References
Jarald E, Joshi SB, Jain DC. “Diabetes and Herbal Medicinesâ€, Iran. J. Pharmaco Therapeut., 2008; 7(1): 97-106.
DeFronzo RA. “Pharmacologic therapy for type 2 diabetes mellitusâ€, Ann. Intern. Med., 1999; 131: 281–303.
Wolff SP. “Diabetes mellitus and free radicals. Free radicals, transition metals and oxidative stress in the aetiology of diabetes mellitus and complicationsâ€, British Med. Bull., 1993; 49: 642- 652.
Ahmed ABA, Rao AS, Rao MV. “In vitro callus and in vivo leaf extract of Gymnema sylvestre stimulate β-cells regeneration and anti-diabetic activity in Wistar ratsâ€, Phytomedicine, 2010; 17: 1033-1039.
Grover JK, Yadav S, Vats V. “Medicinal plants of India with anti-diabetic potentialâ€, J. of Ethanopharmacol., 2002; 81: 81-100.
Singh U, Wadhwani AM, Johri BM. “Dictionary of economic plants of Indiaâ€, In: Jaiswal PL, editor. Indian Council of Agricultural Research New Delhi, 1996; 83:38-39.
Varier PS. “Indian Medicinal Plantsâ€, Orient Longman Pvt. Ltd New Delhi, 2003; 1: 341-43.
Al-Yahiya MA, Al-Meshal IA, Mossa JS, Al-Badr AA and Tarig M. “Saudi plants: A phytochemical and biological approach Riyadhâ€, King Saud university press, 1990; 31- 34.
Fawcett JK, Scott JE. “A rapid and precise method for the determination of ureaâ€, J. Clin. Pathol., 1960; 13:156–159,.
Roeschlau P, Bernt E, Gruber W. “Enzymatic determination of total cholesterol in serumâ€, Zeitschrift fur Klinische Chemie und Klinische Biochemie, 1974; 12: 226.
Allain CC, Poon LS, Chan CS, Richmond W, Fu PC. “Enzymatic determination of total serum cholesterolâ€, Clin. Chem., 1974; 20: 470 – 475.
Friedewald WT, Levy RI, Fredrickson DS. “Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifugeâ€, Clin. Chem., 1972; 18: 499-502.
Bowers LD. “Kinetic serum creatinine assays I. The role of various factors in determining specificityâ€, Clin. Chem., 1980; 26: 551-554.
Wilson BW. “Automatic estimation of urea using urease and alkaline phenolâ€, Clin. Chem., 1966; 12: 360-368.
Trinder P. “Determination of glucose using glucose oxidase with an alternative oxygen acceptorâ€, Ann. Clin. Biochem., 1969; 6: 24-27.
Kavishankar GB, Lakshmidevi N, Mahadeva Murthy S, Prakash HS, Niranjana SR. “Diabetes and medicinal plants-A reviewâ€, Int. J. Pharm. Biomed. Sci., 2011; 2(3): 65-80,.
Swanston-Flat SK, Day C, Bailey CJ, Flatt PR. “Traditional plant treatments for diabetes: studies in normal and streptozotocin diabetic miceâ€, Diabetologia, 1990; 33:462-464.
Chattopadhyay RR. “Possible mechanism of antihyperglycemic effect of Azadirachta indica leaf extractsâ€, J. Ethanopharmacol., 1999; 67: 373-376.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. “Protein measurement with the Folin phenol reagentâ€, J. of Biol. Chem., 1951; 193: 265-275.
Alarcon AFJ, Calzada BF, Hernandez GE, Ruiz AC. “Acute and chronic hypoglycemic effect of Ibervillea sonarae root extracts – IIâ€, J. Ethnopharmacol., 2005; 97: 447- 452.
Singh NS, Geetha M, Amudha P, Chakraborty A. “Evaluation of anti- diabetic activity of methanol extract of Flacourtia jangomas (Lour) in streptozotocin induced diabetic ratsâ€. Int. J. Pharm. Bio. Sci., 2010; 1(3):1-11.
Nanu R Rathod, Havagiray R Chitme, Raghuveer I, Ramesh C. “Hypoglycemic Effect of Calotropis gigantea Linn. Leaves and Flowers in Streptozotocin-Induced Diabetic Ratsâ€, Oman Medical Journal, 2011; 26(2): 104 -108.
Shopna R, Prabakaran K, Ilayaraja S Manivannan R. “Antioxidant, Antimicrobial activities and GC-MS analysis of Calotropis gigantea white flowersâ€, J. of Phytopharma., 2014; 3(6): 405-409.
Bakirel T, Bakirel U, KeleÅŸ OU, Ulgen SG, Yardibi H. “In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbitsâ€, J. Ethnopharmacol., 2008; 116: 64–73.
Reher. G., Slijepcevic M, Krans L. “Hypoglycemic activity of triterpenes and tannins from Sarcopterium spinosum and two sanguisorba speciesâ€, Planta Med., 1991; 57:57-58.
Lee JH, Park JW, Kim JS, Park BH, Rho HW. “Protective Effect of Amomi Semen Extract on Alloxan-induced pancreatic ß-Cell Damageâ€, Phytotherapy. Res., 2008; 22: 86-90.
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