PROTECTIVE EFFECT OF AQUEOUS EXTRACT POMEGRANATE PEEL AGAINST STERIGMATOCYSTIN TOXICITY IN RAT

Abstract

Introduction and Aim: Sterigmatocystin (Stg) a mycotoxin with mutagenic and carcinogenic properties is commonly found as the contaminant in grains and animal feeds. Pomegranate peel is a rich source of antioxidants, flavonoids and other phenolic compounds. So the aim of the current study was to evaluate the protective effects of aqueous extract of red Pomegranate peel against Stg toxicity in liver, kidney, intestine and lung as well as final body weight using male rats.

Methods: Forty eight Sprague-Dawley male rats were divided into six groups (8rats/group) including the control group that fed on a standard diet and water without any treatment, group 2 fed on standard diet plus aqueous extract of RPP (250 mg/rat/day), group 3 fed on standard diet plus aqueous extract of RPP (500 mg/ rat/day), group 4 fed on a standard diet and orally Stg. dissolved at a dose (18µg/rat/day), group 5 fed on a standard diet and Stg  plus aqueous extract of RPP (250mg/day) and group 6 fed on a standard diet and Stg. plus aqueous extract of RPP (500mg/day). At the end of the experimental period, blood samples were collected for serum biochemical analyses. After collecting the blood samples all animals were scarified and dissected samples of liver, kidney, intestine and lung were collected for histological examination.

Results: The total phenols and total flavonoids, compounds in aqueous extract of RPP were 1.38 mg/ml and 680.28 mg/ml, respectively. However, the antioxidant activity amounted to 68.0% in the determination of radical DPPH scavenging activity. On the other hand, results indicated that rat orally Stg plus aqueous extract of RPP with low dose and high doses showed a significant improvement in final body weight compared with group administrated of Stg alone. While, the effect of aqueous extract of RPP on kidney and liver function of rats, the results indicated that the rat orally Stg alone caused significant increased in urea, creatinine and uric acid compared with the control group. The aqueous extract of RPP alone at the two tested doses did not induce any significant changes in the biochemical parameters or the histological picture. The combined treatment showed significant improvements in all tested parameters and histological pictures in the liver tissues. Moreover, this improvement was more pronounced in the group received the high dose of aqueous extract of RPP.

Conclusion: From results it can be concluded that u the aqueous extract of RPP has a potent antioxidant activity and a protective effect against Stg toxicity and this protection was dose dependent.

Keywords: Sterigmatocystin, Red pomegranate peels (RPP), aqueous extract, liver and kidney.