Evaluation of the anti-diabetic effect of the methanol leaf extract and fractions of Dennettia tripetala G. Bak (Annonaceae) in alloxan- induced diabetic mice
This study evaluated the anti-diabetic properties of the methanol extract and fractions of Dennettia tripetala leaves in alloxan diabetic rats. The crude methanol extract (ME), n-hexane (NF), ethyl acetate (EAF), butanol (BF) and water (WF) fractions, were tested for anti-diabetic activity in alloxan-induced diabetic mice. The extracts and its fractions were screened for phytochemical constituents. Glibenclamide was used as the standard anti-diabetic treatment Fasting blood glucose (FBG) levels were determined using a glucometer. Lipid profile parameters were also assessed. Histopathological examination of the pancreas was performed. The presence of glycosides, saponins, reducing sugars, fats and oil, alkaloids, carbohydrates, flavonoids, proteins and tannins were detected in ME, and the fractions. Treatment of the rats with the extract and its fractions reduced FBG levels significantly (p < 0.05) within 10 hours of acute treatment and 14 days short term treatment. The highest reduction was by the crude extract,( ME 500 mg/kg) with percentage reduction of 62.89 %. The study revealed significant (p < 0.05) reductions in serum triglyceride, serum cholesterol and LDL levels by the extracts and its fractions while a significant (p < 0.05) increase in HDL levels was evident . The effect of the extract and fractions on body weight indicated a moderate weight gain. Treatment with high dose of the extract and fractions (500 mg/kg) resulted in marked rejuvenation of the pancreatic β cells. The results of this study suggest that the leaves of Dennetia tripetala are endowed with potent anti-diabetic and antilipidemic properties.
Keywords: Dennetia tripetala, Antidiabetic, Alloxan, Lipid profile, Histopathplogy.
2. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2005: 28: 37-42.
3. Scoppola, A., Montecchi., F. R., Mezinger, G., and Lala, A. Urinary mevalonate excretion rate in type 2 diabetes: Role of metabolic control. Atherosclerosis. 2001; 156: 357-361.
4. Owu, D. U., Antai, A. B., Udofia, K. H., Obembe, A. O., Obasi, K. O. and Eteng, M. U. Vitamin C improves basal metabolic rate and lipid profile in alloxan-induced diabetes mellitus in rats. J Biosci, 2006: 31(5); 575-579.
5. Kesavulu, M. M., Giri, R., Kameswara., R. B. and Apparao., C. Lipid peroxidation and antioxidant enzyme levels in type 2 diabetic with microvascular complications. Diabetic Metab 2000; 26: 387 - 392.
6. Nayeemunnisa, A. Alloxan diabetes-induced oxidative stress and impairment of oxidative defense system in rat brain: neuroprotective effects of Cichorium intybus. Inter J Diabetes Metab. 2009; 17: 105-109.
7. Trivedi, N. A., Majumder, B., Bhatt, J. D., and Hemavathi, K. G. Effect of Shilajit on blood glucose and lipid profile in alloxan– induced diabetic rats. Indian J Pharmacol. 2004; 36: 373-76.
8. Venkatesh, S., Reddy, G. D., Reddy, B. M. and Ramesh. Antihyperglycemic activity of Carulluma attenuate. Fitoterapia. 2003; 74: 274-277.
9. Bailey, C. J. and Day, C., 1989. Traditional plant medicines as treatments for diabetes. Diabetes Care. 1989; 12: 553-564.
10. Grover, J. K., Yadav, S., and Vats, V. Medicinal Plants of India with Antidiabetic Potential. J Ethnopharmacol, 2002; 81: 81-100.
11. Hutchinson, J. and Dalziel, J. M. Flora of West Africa. Crown Agents for Oversea Government and Administrative mill bank, London, 1954. pp 50–51
12. Okiy, G. E. Indigenous Nigerian food plants. J West Afr Sci Asso 1960; 6: 117–121.
13. Oyemitan, I. A. Evaluation of Dennettia tripetala G. Baker (Annonaleae) for central nervous system activities. Ile-Ife, Nigeria: Department of pharmacology, Obafemi Awolowo University; An M. Phil thesis. 2006.
14. Achinewhu, S. G., Ogbonna, C. and Hard, A. D. Chemical composition of indigenous wild herbs, spices fruits and leafy vegetables used as food. Plant Food and Hum Nutr. 1995; 48: 341–388.
15. Lewis, K. and Ausubel F. M. Prospect for plant-derived antibacterials. Nat Biotechnol. 2006; 24(12): 1505–1507
16. Nwaogu, L. A., Alisi, C. S. and Ibegulem, C. O. Evaluation of β – carotene content and presence of some Phytochemical in Five indigenous Fruits. Plant Prod Res J. 2015; 10: 13–14.
17. Kumar, P. P., Kumaravel, S. and Lalitha, C. Screening of antioxidant activity, total phenol and GC–MS study of Vitex negundo. Afr J Biochem Res. 2010; 4(7): 191–195
18. Oyemitan, I. A., Iwalewa, E.O., Akanmu, M. A., and Olugbade, T. A. Antinociceptive and anti-inflammatory effects of essential oil of Dennettia tripetala G. Baker (Annonaceae) in rodents. Afr J Trad Compl Alter Med. 2017; 5: 355–362.
19. Ihekwereme, C. P., Chukwuson, M. A., Erhirhie, E. O. and Uche, G. O. (2016). Preliminary evaluation of the anti-emetic activity of crude methanol extract and fraction of Ocimum gratissimum. J Dev Drugs. 2016; 5 (1): 2329-6631
20. Salim, M. A., Imam, A. A., Dikko, A. A. U., Tanko, Y. and Mohammed, A. Effect of Aqueous Garlic (Ag) Extract of vitex sumplicifolia on blood glucose level, liver glycogen content, lipid profile and antioxidant vitamins in alloxan induced diabetic wistar rats. Bayero J Biomed Sci. 2016; 1(1): 179-157.
21. Ezeigbo, I. Antidiabetic potential of methanolic leaf extracts of Icacina trichantha in alloxan-induced diabetic mice. Inter J Diabetes Dev Countr. 2010; 30(3): 150-152.
22. Tietz, N. W. Clinical guide to Laboratory tests, Second edition W.B. Saunders Company, Philadelphia, USA, 2014; pp 554-556.
23. National Institute of Health Consensus Development Conference Statement (NIHCDCS). Triglyceride, High Density Lipoprotein and Coronary Heart Disease. Washington D.C. 1992
24. Friedewald, W. T., Levy, R. I. and Fredrickson, D. S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative centrifuge. Clin Chem. 2017; 18:499-500.
25. Slaoui, M. and Fiette L. Histopathology procedures: From tissue sampling to histopathological evaluation. Methods Mol Biol. 2014; 691: 69-82.
26. Godwin, A. (2015). Histochemical uses of haematoxylin - A Review. J Phys Chem Solids. 2015; 1:24-34.
27. Krishnakumar, K., Augusti, K. T. and Vijayammal, P. L. Hypoglycemic and anti-oxidant activity of Salacia oblonga wall extract in streptozotozin induced diabetic rats. Indian J Physiol Pharmacol. 2015; 43(3): 510 – 514.
28. Agila K. N. and Kavitha, R. Antidiabetic, antihyperlipidaemic and antioxidant activity of Oxalis corniculata in alloxan induced diabetic mice. Journal of Natural Sciences Research. 2012; 2(7): 9-17.
29. Akah, P. A., Okoli C .O., Ibiam, A. F., Ezike A. C. and Okoye T. C. Evaluation of antidiabeticpotentials of Phyllanthus niruri in alloxan diabetic rats. Afr J Biotechnol.2010; 9: 248-259.
30. Mbaka, G. O., Ogbonnia, S. O., Oyeniran, K. J. and Awopetu, P. I. Effect of Raphiahookeri seed extract on blood glucose, glycosylated haemoglobin and lipid profile of alloxan induced diabetic rats. Bri J Med Medic Res. 2015; 2(4): 621-635.
31. Arika, W. M., Abdirahman, Y. A., Mawia, M. A., Wambua, K. F., Nyamai, D. M., Ogola, P. E., Kiboi, N. G., Nyandoro, H. O., Agyirifo, D. S., Ngugi M. P. and Njagi, E. N. M. In Vivo antidiabetic activity of the aqueous leaf extract of Croton macrostachyus in alloxan induced diabetic mice. Pharmaceut Analyt Acta. 2015 ;2153-2435.
32. Piero, N. M., Kimuni, N. S., Ngeranwa, J. J. N., Orinda., G. O. and Njagi, J. M. Antidiabetic and Safety of Lantana rhodesiensis in Alloxan Induced Diabetic Rats. J Clin Biochem Nutr. 2015; 40: 163-173.
33. Babu, P. V., Liu, D. and Gilbert, E. R. Recent advances in understanding the anti-diabetic actions of dietary flavonoids. J Nutrl Biochem. 2013; 24: 1777-1789.
34. Zheng, T., Shu, G., Yang Z, Mo, S., and Zhao, S. Y. Antidiabetic effect of total saponins from Entada phaseoloides (L.) Merr. in type 2 diabetic rats. J Ethnopharmacol. 2012; 13.
35. Kaleem, M., Kirmani, D., Asif M., Ahmed, Q. and Bano, B. (2015). Biochemical effect of Nigella sativa L. seeds in diabetic rats. Indian J Expt Bio. 2015; 44:745-748.
36. Nwajo, H. U. Efficacy of aqueous leaf extract of Vernonia amygdalina on plasma lipoproteins and oxidative status in diabetic rat model. Nig J Physiol Sci. 2015; 20: 39-42.
37. Aja, P. M., Ibekwe, V. I., Ekpono, E. U., Ugwu, P. C. and Okechukwu, P. C. Effect of ethanol extract of Cajanus cajan leaf on plasma lipid level in albino rats. Inter J Cur Res Acad Rev. 2015
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