study Study of the Hepatoprotective Activity of Polyherbal Formulation on Alcohol Induced Hepatotoxicity
Many traditional systems of medicines employ herbal drugs for the hepatoprotection. Aim of the study was designed to evaluate the hepatoprotective potential of polyherbal formulation against alcohol induced hepatotoxicity in wistar albino rats. Group I animals were treated with 1% CMC for 18 days. Group II, III and IV animals were treated with 1% CMC, polyherbal formulation 180mg/kg/day and silymarin 100mg/kg/day respectively for 18 days and then orally administration with ethanol 3.76 g/kg/day simultaneously for 18 days. After 24 hours of last dosing, the blood was obtained through retro-orbital plexus under light anaesthesia and the animals were sacrificed. Hepatoprotective potential was assessed by various biochemical parameters such as AST, ALT, ALP, LDH, bilirubin, cholesterol, TG and thiopentone sodium induced sleep time. Group III rats showed significant (p<0.01) decrease in AST, ALT, ALP, LDH, bilirubin, cholesterol, TG, liver weight(wt.) and relative liver wt. levels while significant (p<0.01) increase in TP levels as compared to group II rats. Hepatoprotective potential of polyherbal formulation 180mg/kg/day was comparable to that of standard drug silymarin 100mg/kg/day. Results of the study were well supported by histopathological observations. This study confirms that polyherbal formulation possesses hepatoprotective potential comparable to that of standard drug silymarin as it exhibited comparable protective potential against PCM induced hepatotoxicity in albino rats.
Keywords: Polyherbal formulation, Hepatoprotective potential, Alcohol, Hepatotoxicity, Silymarin
2. JC. Kurian, Plants that heals, oriental watchman publishing house, Pune, 2007; 1,53.
3. Joseph T. Dipiro, et al Pharmacoyherapy – A Pathophysiological Approach, 6th Edn, McGraw –Hill Medical Publishing Division PP 729
4. Osawa BH, Kavakishi S. Namiki M. In Kuroda Y, Shanakal DM, Waters MD, editors. Antimutagenesis and anticarcinogenesis mechanism II, 1990; 139-153.
5. L.Pari and K.Karthikesan, Protective effect of caffeic acid against alcohol induced biochemical changes in rats, Fundamental and clinical pharmacology, 2007; 21(4): 355-361.
6. R. Nordmann, C. Ribiere, and H. Rouach, Implication of free radical mechanisms in ethanol-induced cellular injury, Free Radical Biology and Medicine, 1992; 12(3): 219–240.
7. Suzuki Y, Cherian MG, Effect of ethanol on brain metallothionein in transgenic mice. Alcoholism, Clinical and Experimental Result 2000; 24(3): 315-321.
8. Vipul G, Nilesh P, Venkat N, Nandakumar, Gouda TS, hepatoprotective acivity of alcoholic and aqueous extracts of leaves of Tylophora indica (Linn.) in rats. Indian Journal of Pharmacology 2007; 39(1): 43-47.
9. Sadrzadeh SM, Nanji AA, Meydani M. Effect of chronic ethanol feeding on plasma in normal vitamin E- deficient rats. Relashionship to lipid peroxidation, Biochemical Pharmacology, 1994; 47(11): 2005-2010.
10. Babu BH, Shylesh BS, Padikkala J, Antioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia. 2001; 72: 272-277.
11. Ogbera AO, Dasa O, Adeyeye F, Jewo PI, Complementary and alternative medicine use in diabetes mellitus, West Africa Journal of Medicine, 2010; 29(3): 158-162.
12. Kshirsagar AD, Aggrawal AS, Suralkar UR, Hepatoprotective medicinal plants of Ayurveda- A review, Asian journal of Pharmaceutical and Clinical Research. 2010; 4: 1-8.
13. Anbarasu c, Rajkapoor B, Bhat KS, John G, Arul A, Satish K, Protective effect of Pisonia aculeate on thioacetamide induced hepatotoxicity in rats, Asian Pacific Journal of Tropical Biomedicine 2012; 2(7): 511-5.
14. Kumar KJS, Chu FH, Hsie HW, Liao JW, Li WH, Lin JCC, Anthroquinonol from ethanolic extract of mycelium of Antrodia cinnamomea protects hepatic cells from ethanol induced oxidative stress through Nrf-2 activation. Journal of Ethanopharmacology, 2011; 136: 168-77.
15. Okokon JE, Simeon JO, Umoh EE, Hepatoprotective activity of the extract of Homalium letestui stem against paracetamol induced liver injury. Avicenna Journal of Phytomedicine, 2017; 7(1): 27-36.
16. Elberry AA, Shagufta TM, Jaudah AA, Essam AA, Osama MA, Salah AG, Et al. Anti-inflammatory and anti-proliferative activities of date palm pollen (Phoenix dactylifera) on experimantaly induced atypical prostatic hyperplasia in rats, Journal of Inflammation, 2011; 8(40): 1-13.
17. Majid S, Foo S, Luyt B, Xue Z, Yin – Leng T, Chang Y, Intan A Mokhtar, Adopting evidence- based practice in clinical decision making: nurses, perceptions, knowledge, and barriers, Journal of Medical Library Association, 2011; 99(3): 229-36.
18. Leo MA, Arai M, Sato M, Lieber CS, Hepatotoxicity of Vitamin A and ethanol in the rat, Gastroenterology, 1982; 82: 194-205.
19. Ashakumary L, Vijayammal PL, Additive effect of alcohol and nicotine on lipid metabolism in rats. Indian Journal of Experimental Biology, 1993; 31: 270-4.
20. Vilstrup H, Tygstrup N, Glucoregulation in acute liver failure, European Journal of Clinical Investigation, 1985; 16: 193-197.
21. Blancho CD, Gentil S, Alterations of glucose metabolism in chronic liver disease. Diabetes Research and Clinical Practice 1990; 8: 29-36.
22. Rao SK and Mishra S, Anti-inflammatory and hepatoprotective activities of Sida rhombifolia Linn Indian Journal Pharmacology, 1997; 29: 110-116.
23. Saraswat B, Visen PK, PAtnaik GK, Dhawan BN. Anticholestatic effect of Picroliv, active hepatoprotective principle of Picrorhiza kurrooa against CCL4 induced cholestasis, Indian Journal of Experimental Biology, 1993; 31: 316-318.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).