Pharmacognostical standardization and preliminary phytochemical explorations on Salvia hispanica L. seeds
Salvia hispanica L. famous as ‘chia’ is an annual herbaceous plant belonging to family Labiatae, is a richest source of alpha linolenic fatty acid. A detailed examination on chia seeds was performed. The microscopic investigationsshowed the presence of lignified fibres, epidermis cells, aleurone grains, fatty oil globules and lignified sclerides etc. Physicochemical assessment of chia seeds revealed foreign organic matter (0.76%), loss on drying (8.09%), pH (4.39), moisture content (8.96%), swelling index (8.36), foaming index (<100), total ash (7.09%), water soluble ash (1.09%), acid insoluble ash (0.48%), sulphated ash (0.94%),relative density (0.892), refractive index (1.462), acid value (1.862), saponification value (194.29), peroxide value (4.680), acetyl value (169.28) and iodine value (74.62). Elemental analysis was performed using atomic absorption spectroscopy various heavy metals (As, Hg, Pb, Cd) and other elements (Cu, Zn, Fe and Mg) all were within limits. Chia seeds crude material as well as its extract showed no microbial contamination. High performance thin layer chromatographyfingerprinting profile of chia seeds fixed oil showed the presence of alpha linolenic acid. Work on standardization assumes imperative consequence. However,wide-ranging literature survey on this plant revealed that, more research work is required to update the standardization parameters. Therefore, findings of this study will facilitate quality control and presence of the various phytoconstituents in plant seed.
Keywords: Salvia hispanica, Standardization, Pharmacognostical, Physicochemical, HPTLC fingerprints.
2. Amponsah IK, Mensah AY, Otoo A, Mensah MLK, Jonathan J. Pharmacognostic standardisation of Hillerialatifolia(Lam.) H. Walt. (Phytolaccaceae), Asian Pacific Journal of Tropical Biomedicine, 2014; 4(12):941-946.
3. Sharma N, Singh S, Singh SK. Pharmacognostical standardization and preliminary phytochemical investigations on Acacia auriculiformis A. Cunn. Ex. Benth stem bark. Journal of Medicinal Plants. 2017; 5(1):398-402.
4. Ullah R, Nadeem M, Khalique A, Imran M, Mehmood S, Javid A, Hussain J. Nutritional and therapeutic perspectives of Chia (Salvia hispanica L.): a review. Journal of food science and technology. 2016; 53(4):1750-1758.
5. Segura-Campos MR, Ciau-Solís N, Rosado-Rubio G, Chel-Guerrero L, Betancur-Ancona D. Physicochemical characterization of chia (Salvia hispanica) seed oil from Yucatán, Méxic,. Agricultural Sciences, 2014; 5(03):220.
6. Scapin G, Schmidt MM, Prestes RC, Rosa CS. Phenolics compounds, flavonoids and antioxidant activity of chia seed extracts (Salvia hispanica) obtained by different extraction conditions, International Food Research Journal, 2016; 23:2341-2346.
7. Dugganaboyana G, Palanisamy C, Kiran K, Sridhar M, Velliyur K. Dietary evaluation, antioxidant and cytotoxic activity of crude extract from chia seeds (Salvia hispanica L.) against human prostate cancer cell line (PC-3), International Journal of Pharmacognosy and Phytochemical Research, 2016; 8:1358-1362.
8. Gazem RA, Puneeth HR, Madhu CS, Sharada AC. Physicochemical Properties and in Vitro Anti-Inflammatory Effects of Indian Chia (Salvia hispanica L.) Seed Oil, Journal of Pharmacy and Biological Science, 2016; 11:1-8.
9. DivyapriyaGK, Veeresh DJ, Yavagal PC. Evaluation of antibacterial efficacy of chia (Salvia hispanica) seeds extract against porphyromonasgingivalis, fusobacterium nucleatum and aggregatibacteractinomycetemcomitans-an in-vitro study, International Journal of Ayurveda and Pharma Research, 2016; 4:22-26.
10. de Souza AP, Nascimento LM, de Lima VC, de Carvalho FM, dos Santos EA, de Araújo Morais AH. Anti-tryptic activity in seed and food product of chia (Salvia hispanica L.), DEMETRA: Food, Nutrition & Health, 2017; 12:319-331.
11. Gazem RA, Puneeth HR, Sharada AC. Hypolipidemic, hypoglycemic and antiproliferateproperties of chia seed oil and its blends with selected vegetable oils-An in-vitro study, IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB), 2016; 2: 33-39.
12. The Ayurvedic Pharmacopoeia of India. New Delhi: Department of yoga and Naturopathy, Unani and Siddha, Ministry of Health and Family Welfare, Govt. of India. 1996; 1st edition: 137.
13. Indian Pharmacopoeia. Ghaziabad, India: The Indian Pharmacopoeia Commission, Government of India. 2010; 6th edition: 82-171.
14. AOAC. AOAC official methods of analysis. Association of Official Analytical Chemists. Arlington: Virginia. 1990: 15th edition: 84-85.
15. Hseu ZY. Evaluating heavy metal contents in nine composts using four digestion methods. Bioresource Technology. 2004; 5:53-59.
16. Sajitha MK, Thoppil JE. Phytochemical evaluation and in vitro antioxidant studies of selected species of Gomphostemma Wall. ex Benth. from Western Ghats. Journal of Drug Delivery and Therapeutics. 2018; 8:32-37.
17. Baired RM, Hodge NA, Denyer SP. Hand book of microbial quality control. 2000: 22-36.
18. WHO guidelines quality control methods for medicinal plant materials. WHO Geneva, New Delhi: A.I.T.B.S. Publishers and Distributors; 1998: 60-70.
19. Ichihara KI, Fukubayashi Y. Preparation of fatty acid methyl esters for gas-liquid chromatography, Journal of lipid research, 2010; 51(3):635-640.
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