Costus afer modulates the activities of glycolytic and gluconeogenic enzymes in streptozotocin induced diabetic rats
Abstract
Background: Glycolytic enzymes (Hexokinase and α-glucosidase) and gluconeogenic enzymes (Glucose-6-phosphatase and fructose-1,6-disphosphatase) control carbohydrate metabolism and hence glucose hemostasis, an important approach for the control of diabetes. The present study was carried out to evaluate the effect of hydro-ethanolic leaf extract of Costus afer on these enzymes.
Materials and methods: Streptozotocin-induced diabetic rats were administered hydro-ethanolic leaf extract of Costus afer (250, 500 and 1000 mg/kg body weight) orally for 8 weeks. Effect of the extract was monitored on the decrease in glycemia and the modulatory activities on glycolytic and gluconeogenic enzymes activities. The pancreas was also immunohistochemically examined for regeneration of the Islet cells.
Results: Hydro-ethanolic extract significantly decreased the blood glucose concentration, serum glucose-6-phosphatase and fructose-1,6-disphosphatase activities which were associated with an increase in the activity of hexokinase in the liver and kidney. immunohistochemical examination of the pancreas, revealed the preservation and regeneration of the islet of the Langerhans cells.
Conclusion: Thus, C. afer acts against hyperglycemia modulating the activities of glucose-6-phosphatase, fructose-1,6-bisphosphatase and hexokinase, and regeneration of β-cells.
Keywords:
Costus afer, gluconeogenic enzymes, glycolytic enzymes, streptozotocin, α-glucosidaseDOI
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