Subcronic Toxicity Effect of Ethanol Extract of Bitter Melon Fruit (Momordica charantia L.) on Kidney Function and Histopathological Changes in Wistar Rat

  • Welly Ratwita Departement of Pharmacology Faculty of Medicine Jenderal Achmad Yani University, INDONESIA

Abstract

Bitter melon is a fruit that was famous for its bitter taste and contains chemical compounds that are proven to have effects such as antidiabetic, anti-inflammatory, antipyretic, and antioxidant. One of the prerequisites of a plant to be developed into a drug that is by testing the subchronic toxicity. This study goal to determine the presence of toxic effects that are not detected in the acute toxicity test. A total of 40 wistar rats were divided into group control and 3 treatment groups. Each group consisted of 5 male rats and female rats. Group 1 (control) is only given food (pellets) and drinking water. Group 2, 3 and 4 groups were given ethanol extract of bitter melon pulp (Momordica charantia L.) with multilevels doses 250 mg/kgbw, 500 mg/kgbw, and 1000 mg/kgbw. The observations made for 28 days included the number of death animals, relative organ weight (ROW), ureum, creatinine levels and histopatological changes in kidney. There were no deaths in the entire group, decreasing ureum (p=0,022), creatinine levels (p=0,033) in male group. While in female group ureum decrease not significantly (0.878) and creatinine (0.845). There were no significant changes in ROW (p=0,370 and p=0,394), but there were changes in microscopic structure. In conclusion ethanol extract of bitter melon fruit (Momordica charantia L.) at a  of 250 mg/kgbw, 500 mg/kgbw, and 1000 mg/kgbw does not showed any toxic effects on kidney function and relative organ weight. But microscopically, the administration of ethanol extract of bitter melon fruit caused a toxic effect on the kidney tubules in both male and female groups.


Keywords: Momordica charantia L., sub chronic toxicity, ureum, creatinine, histopathological change

Keywords: Momordica charantia L., sub chronic toxicity, ureum, creatinine, histopathological change

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Author Biography

Welly Ratwita, Departement of Pharmacology Faculty of Medicine Jenderal Achmad Yani University, INDONESIA

Departement of Pharmacology Faculty of Medicine Jenderal Achmad Yani University, INDONESIA

References

1. Ministry of Trade of the Republic of Indonesia. Traditional herbal medicine. Export News 2014; 005:1–20.
2. Sutoyo. Indonesia's biodiversity. Science World 2010; 10:101–106.
3. Jia S, Shen M, Zhang F, Xie J. Recent advances in Momordica charantia : Functional components and biological activities. International Journal of Molecular Sciences 2017; 18:2555. https://doi.org/10.3390/ijms18122555
4. Afifah UN. Antidiabetic activity test of 96% ethanol extract of bitter melon (Momordica charantia L.) against alloxan-induced wistar male rats. Surakarta: Faculty of Pharmacy, Muhammadiyah Surakarta University. 2017.
5. Parawansah, Wahyuni, Mahmudah Z. Test of antipyretic and anti-inflammatory effects of ethanol extract of bitter melon (Momordica charantia L.) on male mice. Kendari: Faculty of pharmacy, Universitas Halu Oleo. 2016.
6. Regulation of the Head of the Food and Drug Supervisory Agency of the Republic of Indonesia No 7. Guidelines for in vivo non-clinical toxicity tests. PerKBPOM. Jakarta. 2014.
7. Guyton AC, Hall JE. Guyton and Hall. Nedical physiology textbook. Ed 12. Singapore: Elsevier; 2011. p 325-45.
8. Food and Drug Supervisory Agency. Guidelines for In Vivo Nonclinical Toxicity Testing. 2014(June): p 1-2.
9. OECD Guidelines for the Testing of Chemicals: Acute Oral Toxicity-Fixed Dose Procedure, OECD/OCDE 420. Adopted: 17th December 2001
10. American Veterinary Medical Association. Guidelines for the euthanasia of animals: 2013 Edition. AVMA. 2013.
11. Medical Laboratory Technology Teaching Materials. 2017. Cytohistotechnology. Jakarta: Ministry of Health of the Republic of Indonesia. p:139-58.
12. Siagian M, Jusuf AA, Handini M. Effect of monosodium glutamate exposure on rat kidney function and histology and its changes after cessation of exposure. Journal Indonesia Medical Association. 2014; 64(7).
13. Priyanto. Toxic effect of a substance. In: Sunaryo H, editor. Toxicology (Mechanisms, Antidote Therapy, and Risk Assessment). Depok. Leskonfi. 2009. hal. 37-47.
14. Anilakumar KR, Kumar GP, Ilaiyaraja N. Nutritional, pharmacological and medicinal properties of Momordica charantia L. Int. J. Nutr. Food Sci. 2015; 4(1):75-83 https://doi.org/10.11648/j.ijnfs.20150401.21
15. Praptiwi, Nurkanto A, Wulansari D, Agusta A. Acute oral toxicity of two compounds bisantraquinone (+)-2,2'-epicytocycrine A and (+)-1,1'-bislunatine. Biology News. 2015; 14(1):11-18.
16. Sugihartini N, Fajri NA. Histopathological Description of Liver and Kidneys of Balb/c Mice after Administration of Green Tea Extract Cream (Camellia sinensis L.). Yogyakarta: Faculty of Pharmacy, Ahmad Dahlan University. 2016.
17. Almunawati, Budiman H, Aliza D. Histopathology of the kidneys of white rats (Rattus norvegicus) injected with formalin. Banda Aceh: Faculty of Veterinary Medicine, Syiah Kuala University. 2017.
18. Nasikha FI, Subchronic Toxicity Test of Ethanol Extract of Matoa Leaves (Pometia Pinnata) With Bun, Creatinine and Kidney Histopathology Parameters in Wistar Strain Rats. Surakarta: Faculty of Pharmacy, Setia Budi University. 2018
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Ratwita W. Subcronic Toxicity Effect of Ethanol Extract of Bitter Melon Fruit (Momordica charantia L.) on Kidney Function and Histopathological Changes in Wistar Rat. JDDT [Internet]. 21Nov.2021 [cited 29Mar.2024];11(6):143-9. Available from: https://www.jddtonline.info/index.php/jddt/article/view/5168