Development and Validation of a RP-HPLC Method for the Simultaneous estimation of Amoxicillin, Omeprazole and Tinidazole in fixed dose combinations

  • Basant Lal Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India
  • Manish Jaimini Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India
  • Devesh Kapoor Department of Pharmacy, Dr. Dayaram Patel Pharmacy College, Bardoli, Gujarat-394601, India

Abstract

New liquid chromatographic technique was established for the simultaneous estimation of tinidazole, omeprazole and amoxicillin in the fixed dose combination (HP-KIT by Sun Pharma). RP-HPLC elution has performed by using the Phenomenex Luna column (250 mm x 4.6 mm) having internal diameter and the packing material of size 5µm) in isocratic mobile phase of solution A: acetonitrile at a ratio of 80:20 v/v (Solution A consists of Buffer: Acetonitrile: Methanol: Triethylamine in the ratios of 68:22:10:0.01 respectively). The selected flow rate was kept as 1 ml/min and selected wavelength was 230 nm was for detection of the drugs in UV detector. As per the ICH guidelines, the method validation was carried out. Moreover, the different parameters of method such as precision, specificity, linearity, robustness and accuracy were established. The time of retention for the tinidazole, amoxicillin, and omeprazole were 4.021 2.324, and 7.332 minutes respectively. The RP-HPLC approach was robust and accurate, so it is appropriate for repetitive assay of drugs and quality control. This method is effectively used for the assessment of marketable dosage form preparation.


Keywords: RP-HPLC, Amoxicillin, Tinidazole, Omeprazole, Method Development, Method Validation.

Keywords: RP-HPLC, Amoxicillin, Tinidazole, Omeprazole, Method Development, Method Validation

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Author Biographies

Basant Lal, Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India

Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India

Manish Jaimini, Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India

Department of Pharmaceutical Sciences, Maharishi Arvind University, Mundiaramsar, Jaipur, Rajasthan-302041, India

Devesh Kapoor, Department of Pharmacy, Dr. Dayaram Patel Pharmacy College, Bardoli, Gujarat-394601, India

Department of Pharmacy, Dr. Dayaram Patel Pharmacy College, Bardoli, Gujarat-394601, India

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1.
Lal B, Jaimini M, Kapoor D. Development and Validation of a RP-HPLC Method for the Simultaneous estimation of Amoxicillin, Omeprazole and Tinidazole in fixed dose combinations. JDDT [Internet]. 15Oct.2021 [cited 1Dec.2021];11(5-S):57-2. Available from: http://www.jddtonline.info/index.php/jddt/article/view/5020