Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats

  • Dhaval M M Patel SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India
  • Advaita B Patel SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India
  • Bhumi R Patel SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India
  • Riddhi D Trivedi SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India
  • Deepa R Patel Kalol Institute of Pharmacy, Kalol, Gandhinagar, Gujarat, India

Abstract

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis seeds on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis seedss significantly decreased neutrophil adhesion. The results indicate that the seed of Cassia occidentalis is endowed with protected neutrophil adhesion.


Keywords: Cassia occidentalis, Neutrophil, analysis of variance

Keywords: Cassia occidentalis, Neutrophil, analysis of variance

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Author Biographies

Dhaval M M Patel, SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

Advaita B Patel, SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

Bhumi R Patel, SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

Riddhi D Trivedi, SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

SAL Institute of Pharmacy, Opp-Science city, Ahmedabad, Gujarat, India

Deepa R Patel, Kalol Institute of Pharmacy, Kalol, Gandhinagar, Gujarat, India

Kalol Institute of Pharmacy, Kalol, Gandhinagar, Gujarat, India

References

1. http://google/WHO, 1998.
2. Kay AB. Asthma and inflammation. J Allergy Clin immunol. 1991; 87:893-910.
3. Bethesda. NHLBI: National Asthma Education and Prevention Program, Expert Panel report 2, Guidelines for the diagnosis and management of Asthma. NIH Publication. 1997; 97:4051.
4. Barar FSK. Drugs acting on respiratory system. In: Essentials of Pharmacotherapeutics. 3rd edition. 2003; 544-552.
5. Fireman P. Understanding asthma Pathophysiology. Allergy Asthma Proc. 2003; 24 (2):79-83.
6. http:// www.google/ WHO fact sheet, 2000.
7. Walker R, Edwards C. Clinical pharmacy and therapeutics. 2nd edition. 1999: 351-365.
8. Bangoriya U.V., Tank H.M., Patel D.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in clonidine induced mast cell degradation in Rats. JPSBR. 2016; 6 (1):120-123.
9. Patel D.M., Tank H.M., Bangoriya U.V., Tank, P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in carrageenan induced paw edema in Rats. JPSBR. 2016; 6 (1):157-161.
10. Bangoriya U.V., Tank H.M., Patel D.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in carrageenan induced paw edema in rats. Pharma Science Monitor: An International Journal Of Pharmaceutical Sciences. 2015; 6 (3):65-72.
11. Bangoriya U.V., Tank H.M., Patel D.M., Tank, P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in histamine induced bronchospasm on guinea pigs. Pharma Science Monitor: An International Journal Of Pharmaceutical Sciences. 2015; 6 (4):118-124.
12. Patel D.M., Bangoriya U.V., Tank H.M., Tank, P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in clonidine induced mast cell degradation in Rats. JPSBR. 2017; 7 (5):346-349.
13. Patel D.M., Patel A.B., Trivedi R.D., Parmar V.J., Bangoriya U.V. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis leaves in neutrophil adhesion test in rats. JDDT. 2019; 9 (4s):1218-1221.
14. Balsitis M, Morrell K, Mahida Y, Hawkey CJ. Cell adhesion molecule expression by colonic mucosa in ulcerative colitis. Eur J Gastroenterol Hepatol. 1994; 6:351-358.
15. Dippold W, Wittig B, Schwaeble W, Mayet W, Buschenfelde M. Expression of intercellular adhesion molecule 1 (ICAM-1, CD54) in colonic epithelial cells. Gut. 1993; 34:1593-1597.
16. Susan CF, Qutayba H. Images in allergy and immunology: neutrophils in asthma. Journal of Allergy and Clinical Immunology. 2007; 119:1282-1286.
17. Madara JL. Migration of neutrophils through epithelial monolayers. Trends in Cell Biology. 1994; 4:4-7.
18. Nathan CF. Neutrophil activation on biological surfaces: massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes. J Clin Invest. 1987; 80:1550-1560.
19. Opal SM, Palardy JE, Marra MN, Fisher CJ, McKelligon BM, Scott RW. Relative concentrations of endotoxin binding proteins in body fluids during infection. Lancet. 1994; 344:429-431.
20. Soussi-Gounni A, Koussih L, Nutku E, Lamkhioued B, Nicolaides NC, Levitt RC. Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: role in IL-8 release. Journal of Immunology. 2001; 166:2768-2774.
21. Soussi-Gounni A, Lamkhioued B, Koussih L, Ra C, Renzi PM, Hamid Q. Human neutrophils express the high-affinity receptor for immunoglobulin E (Fc epsilon RI): role in asthma. FASEB J. 2001; 15:940-949.
22. Adams DH, Shaw S. Leucocyte-endothelial interactions and regulation of leucocyte migration. Lancet. 1994; 343:831-836.
23. Patel D.M., Trivedi R., Patel H. Formulation and Evaluation of Bi-Layer Tablets of Ketorolac Tromethamine. Journal of Drug Delivery and Therapeutics. 2021; 11(1):36-41. DOI 10.22270/jddt.v11i1.4487
24. Nakarani P.P., Patel D.M. Development and Validation of Stability Indicating HPTLC Method for Estimation of Acebrophylline in Their Dosage Form. IJPRT. 2013; 3 (1):1-4.
25. Patel A., Patel D., Patel D., Sheth A., Shah U.J. Method Development and Validation for Simultaneous Estimation of Benidipine Hydrochloride and Metoprolol Succinate in Tablet. Journal of Drug Delivery and Therapeutics. 2019; 9(6-s):28-33. DOI 10.22270/jddt.v9i6-s.3692
26. Prescott SM, Zimmerman GA, McIntyre TM. Platelet activating factor. J Biol Chem. 1982; 1990 (17):381-384.
27. Dianzani C, Brunelleschi S, Viano I, Fantozzi R. Adenosine modulation of primed human neutrophils. European Journal of Pharmacology. 1994; 263: 223-226.
28. Cronstein BN, Levin RI, Philips M, Hirschhorn R, Abramson SB, Weissman G. Neutrophil adherence to endothelium is enhanced via adenosine Al receptors and inhibited via adenosine A2 receptors. Journal of Immunology. 1992; 148:2201-2206.
29. Meenan J, Mevissen M, Monajemi H, Radema SA, Soule HR, Moyle M, Tytgat GNJ, van Deventer SJH. Mechanisms underlying neutrophil adhesion to apical epithelial membranes. Gut. 1996; 38:201-205.
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Patel DM, Patel A, Patel BR, Trivedi RD, Patel D. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats. JDDT [Internet]. 26Feb.2021 [cited 18Apr.2021];11(1-s):117-20. Available from: http://www.jddtonline.info/index.php/jddt/article/view/4569