Evaluation of Efficacy of Cucumis melo in Gentamycin and CPD Induced Urolithiasis on Rats
Evaluation of the efficacy of methanolic extract of Cucumis melo in urolithiasis induced by gentamycin and calculi producing diet on Wistar rats. Gentamycin (40 mg/kg, subcutaneously) and calculi-producing diet (CPD) was fed to induce urolithiasis on Wistar rats. The effect of oral administration of methanolic extract of Cucumis melo seed on calcium oxalate urolithiasis has been studied and is compared with the effect of oral administration of Cystone as standard on Wistar rats. Gentamycin and CPD feeding resulted in hyperoxaluria and calcium oxalate deposition as well as increased renal excretion of calcium and oxalate. Supplementation with methanolic extract of Cucumis melo seed reduced the elevated urinary oxalate, showing a regulatory action on endogenous oxalate synthesis. The results indicate that the seed of Cucumis melo is endowed with antiurolithiatic activity.
Keywords: C. melo, Hyperoxaluria, calcium oxalate deposition, cystone, hyperoxaluria, analysis of variance
2. Harvey J.A., Hill K.D., Pak C.Y. Similarity of urinary risk factors among calculus-forming patients in five regions of the United States. J. Lithotr. Calculus Dis., 1990; 2:124-132.
3. Herring L.C. Observations on the analysis of ten thousand urinary calculi. J. Urol., 1962; 88:545-562.
4. Daudon M., Donsimoni R., Hennequin C., Lacour B., Moel G., Donsimoni R. Sex- and age-related composition of 10617 calculi analyzed by infrared spectroscopy. Urol. Res., 2005; 23:319-326.
5. Mandel N.S., Mandel G.S. Urinary tract calculus disease in the United States veterans population. I. Geographic frequency of occurrence. J. Urol., 1989; 142:1513-1515.
6. Patel D.M., Patel A.B., Trivedi R.D., Parmar V.J., Bangoriya U.V. Evaluation of the effect of hydroalcoholic extract of Cassia occidentalis leaves in neutrophil adhesion test in rats. Journal of Drug Delivery and Therapeutics. 2019; 9(4s):1218-1221. DOI 10.22270/jddt.v9i4-s.3940
7. Saita A., Bonaccorsi A., Motta M. Calculus composition: where do we stand? Urol. Int. 2007; 79(1):16-19.
8. Al-Mamari S.A. Complications of urolithiasis, Urolithiasis in Clinical Practice. Springer Publication, 2017; 121-129.
9. Patel A., Patel D., Patel D., Sheth A., Shah U.J. Method Development and Validation for Simultaneous Estimation of Benidipine Hydrochloride and Metoprolol Succinate in Tablet. Journal of Drug Delivery and Therapeutics. 2019; 9(6-s):28-33. DOI 10.22270/jddt.v9i6-s.3692
10. Patel D.M., Trivedi R., Patel H. Formulation and Evaluation of Bi-Layer Tablets of Ketorolac Tromethamine. Journal of Drug Delivery and Therapeutics. 2021; 11(1):36-41. DOI 10.22270/jddt.v11i1.4487
11. Bangoriya U.V., Tank H.M., Patel D.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in clonidine induced mast cell degradation in Rats. JPSBR. 2016; 6 (1):120-123.
12. Patel D.M., Tank H.M., Bangoriya U.V., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in carrageenan induced paw edema in Rats. JPSBR. 2016; 6 (1):157-161.
13. Bangoriya U.V., Tank H.M., Patel D.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in carrageenan induced paw edema in rats. Pharma Science Monitor: An International Journal Of Pharmaceutical Sciences. 2015; 6 (3):65-72.
14. Bangoriya U.V., Tank H.M., Patel D.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis in histamine induced bronchospasm on guinea pigs. Pharma Science Monitor: An International Journal of Pharmaceutical Sciences. 2015; 6 (4):118-124.
15. Patel D.M., Bangoriya U.V., Tank H.M., Tank P.H. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in clonidine induced mast cell degradation in Rats. JPSBR. 2017; 7 (5):346-349.
16. Patel D.M., Patel A.B., Trivedi R.D., Parmar V.J., Bangoriya U.V. Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis leaves in neutrophil adhesion test in rats. Journal of Drug Delivery and Therapeutics. 2019; 9(4s):1218-1221. DOI 10.22270/jddt.v9i4-s.3940
17. Patel D.M., Lumbhani A.N. Evaluation of the efficacy of methanolic extract of Foeniculum vulgare in urolithiasis on Wistar rats. Int J Pharmacy Res and Tech, 2013; 3(2):12-15.
18. Medeiros D.M., Mustafa M.A. Proximate composition, mineral content and fatty acids of cat fish (Ictalurus punctatus rafinesque) for different seasons and cooking methods. J. Food Sci., 1985; 50:585-588.
19. Hodgkinson A., Williams, A. An improved colorimetric procedure for urine oxalate. Clinica Chimica Acta, 1972; 36:127-132.
20. Patel A.B., Patel D.R., Patel D.M., Patel K.S., Patel P. Stability indicating HPLC method development and validation of Enrofloxacin and Bromhexine HCl in combined dosage form. Aegaeum Journal. 2020; 8 (5):443-457.
21. Khan S.R., Hackett R.L. Membrane induced calcium oxalate crystal nucleation. Urol Res, 1988; 16:185.
22. Nakarani P.P., Patel, D.M. Development and Validation of Stability Indicating HPTLC Method for Estimation of Acebrophylline in Their Dosage Form. IJPRT. 2013; 3 (1):1-4.
23. Patel D.M., Patel D., Patel A., Sheth A., Shah U.J. Method Development and Validation for Simultaneous Estimation of Benidipine Hydrochloride and Metoprolol Succinate in Tablet. Journal of Drug Delivery and Therapeutics. 2019; 9(6-s):28-33. DOI 10.22270/jddt.v9i6-s.3692
24. Chahwala S.B., Harpur E.S. Gentamicin–induced hypercalciuria in the rat. Acta Pharmacol Toxicol, 1983; 53:358.
25. Baumann M.J. Calculus prevention: why so little progress? Urol Res, 1998; 26:77-81.
26. Baskar R., Malini M.M., Varalakshmi P., Balakrishna K., Rao, R.B. Effect of Lupeol isolated from Crataeva nurvala stem bark against free radical induced toxicity in experimental urolithiasis. Fitoterapia, 1996; 2:121-125.
27. Robertson W.G., Peacock M. The course of idiopathic calcium disease: hypercalciuria or hyperoxaluaria. Nephron, 1980; 26:105-110.
28. Borghi, L, Meschi T., Guerra A., Novanni A. Randomized prospective study of a nonthiazide diuretic, indapamide, in preventing calcium calculi recurrences. J Cardiovasc Pharmacol, 1993; 22:78-86.
29. Marshall R.W., Robertson W.G., Nonograms for the estimation of the saturation of urine with calcium oxalate, calcium phosphate, magnesium ammonium phosphate, uric acid, sodium acid urate. Clin Chim Acta, 1976; 72:253-257.
30. Pak C.Y.C., A cautious use of cellulose phosphate in the management of calcium nephrolithiasis. Invest Urol, 1981; 19:187-190.
31. Jha A.N., Patel D.M. Role of Cannabinoid receptors in psychological disorder. Borneo Journal of Pharmacology. 2020; 3 (4):199-208.
32. Grases F., March J.G., Ramis M., Costa-Bauza A. The influence of Zea mays on urinary risk factors for kidney calculi in rats. Phytother Res, 1993; 7:146-149.
33. Silva H.J.R.L., Anusari L.B.W., Ratnasooriya W.D., Pathirana R.N., Widanagamage R.D. In vitro antibacterial activity and the minimum inhibitory concentration of aqueous seeds extract of Cucumis melo L. grown in Sri Lanka on common urinary tract infective bacteria. Journal of Pharmacognosy and Phytochemistry, 2018; 7(3):686-690.
34. Grases F., Ramis M., Costa-Bauza A., March J.G. Effect of Herniaria hirsuta and Agropyron repens on calcium oxalate urolithiasis in rats. J Ethnopharmacol, 1995; 45:211-214.
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