Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

  • Meriem Hamoudi Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Djouher Amroun Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Seddik Khennouf Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Saliha Dahamna Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Abstract

Ephedra nebrodensis, is a medicinal plant, traditionally in Algeria used for the cure of various ailments such as hepatic disorders. On this basis in present study the phytochemical screening and in vitro antioxidant activity of various fractions (n-hexane, chloroform, ethyl acetate, n-butanol and water) of ethanol extract obtained by maceration from aerial parts of E. nebrodensis were investigated. Different fractions were accessed for their yield percentage, total polyphenol content (TPC), total flavonoid content (TFC), total tannins content (TTC) and antioxidant potential (DPPH - 2,2-diphenyl-1-picrylhydrazyl; ABTS - 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), reducing power assay (FRAP - ferric reducing antioxidant power activity),and bleaching of β-carotene test . Results showed that ethyl acetate fraction (EF) represents the highest amount of total polyphenols, flavonoids and tannins with 269.669 ± 0.031 µg gallic acid equivalent/mg of DE, 44.507 ± 0.003 µg quercetin equivalents/mg of DE and 228.487 ± 1.362 µg tannic acid equivalents/mg of DE, respectively. In antioxidant assays, ethyl acetate fractions (EF) showed the strongest DPPH and ABTS antioxidant (IC50: 0.009 and 0.004 µg/mL) and FRAP potential (EC50: 0.005 ± 0.000 µg/mL), which significantly correlate to its high content of polyphenolics, flavonoids and tannins. Further, fractions were rich in phenolic compounds. The present results support the possible use of the ethyl acetate fraction from E. nebrodensis in pharmaceutical industries as a natural antioxidant.


Keywords: Ephedra nebrodensis, polyphenols, flavonoids, tannins, antioxidant capacity.
Keywords: Ephedra nebrodensis, polyphenols, flavonoids, tannins, antioxidant capacity

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Author Biographies

Meriem Hamoudi, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Djouher Amroun, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Seddik Khennouf, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Saliha Dahamna, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

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Hamoudi M, Amroun D, Khennouf S, Dahamna S. Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis. JDDT [Internet]. 5Nov.2020 [cited 28Mar.2024];10(5-s):314-9. Available from: https://www.jddtonline.info/index.php/jddt/article/view/4377