Antioxidant, antimicrobial and anti-inflammatory activities development of methanol extract of Cyclamen africanum B. et R., growth in Jijel - Algeria
Abstract
The genus Cyclamen L. (Primulaceae) is represented by 20 species, where inter alia Cyclamen africanum B. et R, is the only endemic species presented in Algeria. The study of their biological potentialities was evaluated through antioxidant, antimicrobial and anti-inflammatory activities. The antioxidant activity was evaluated by DPPH and the reducing power essay, the antimicrobial activity was tested with three bacterial strains and one yeast (Staphylococcus aureus ATCC25923, Klebsiella pneumonia ATCC700603, Bacillus subtilis ATCC6633 and Candida albicans ATCC1024) and anti-inflammatory activity with the Human Red Blood Cell (HRBC) membrane stabilization method. The results demonstrate a perfect antioxidant activity and an excellent anti- inflammatory power at very small concentrations.
Keywords: Cyclamen; crude extract; reducing power; antimicrobial; HRBC.
Downloads
References
[2] Emre O, Samed K, Olcay Dinc D, and Ramazan M. Huseyin Cetin. Larvicidal activity of Cyclamen (Myrsinaceae) extracts against the larvae of West Nile virus vector Culex pipiens L. (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine. 2013; 449-452.
[3] Grey-Wilson C, Cyclamen A, Guide for Gardeners. Horticulturists and Botanists. New edition. Batsford, London. 2003.
[4] Yildirim M, Akcal A, and Kaynas K, The response of Cyclamen hederifolium to water stress induced by different irrigation levels. Afr. J. Biotechnol. 2009 ; (8):1069 – 1073.
[5] Speroni E, Cervellati R, Costa S, Dall’Acqua, S, Guerra M C, Panizzolo C, Utan A. and Innocenti, G, Analgesic and antiinflammatory activity of Cyclamen repandum S et S Phytother.; Res, 2007 ; (21):684–689.
[6] Leporatti M L, Ivancheva S. Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy. J. Ethnopharmacol, 2003 ; 87:123–142.
[7] Quezel P and Santa S La nouvelle flore de l’Algerie et des regions désertiques meridionales. Editions du centre national de la recherche scientifique. Paris, France, 1963, p 1171.
[8] Neda S L, Neda M M D, Jelena M I. and Biljana N B Antioxidant properties of Galium verum L. (Rubiaceae) extracts, Cent. Eur. J. Biol. 2010; 331–337.
[9] Li WD, Wei C L, White P J. and Beta T. High-amylose corn exhibits better antioxidant activity than typical and waxy genotypes. Journal of Agricultural and Food Chemistry, 2007; 55: 291-298.
[10] Bahorun T, Gressier B, Trotin F, Brunete C, Dine T , Vasseur J, Gazin J C, Pinkas M, Luycky M and Gazin M. Oxigen species scavenging activity of phenolic extract from howthorn fresh plant organs and pharmaceutical preparation. Arzneim Forsch / Drug Res,1996; 1-6.
[11] Hanato T, Kagawa H, Yasuhara T and Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chemical & Pharmaceutical Bulletin, 1998; 2090–2097.
[12] R IBettaieb S, Bourgou I, Ben Slimen Debez I, Jabri Karoui I, Hamrouni Sellami K, Msaada , F Limam and B.Marzouk. Food Bioprocess Techno, 2011- 1007.
[13]M Oyaizu. 1986. Studies on products of browningreactions: antioxidative activities of products of browning reaction prepared from glucosaminutese, Japanese Journal of Nutrition, 1986; 307–315.
[14] SJ Huang and JL Mau. Antioxidant properties of methanolic extracts from Agaricus blazei with various doses of ɣ-irradiation. Swiss Society of Food Science and Technology, 2006; 39:707–716.
[15] NCCLS (National Committee for Clinical Laboratory Standards). Performance standards for antimicrobial susceptibility testing. Wayne Pa. 9th International Supplement, 1999; M100-S9.
[16] NCCLS (National Committee for Clinical Laboratory Standards). Performance standards for antimicrobial disk susceptibility test. Wayne Pa. 6th ed. Approved Standard, 1997; M2-A6.
[17] Alves TMA, Silva AF, Brandão M, Grandi TSM, Smânia EFA, Smânia Jr A and Zani CL. Biological screening of Brazilian medicinal plants. Memórias do Instituto Oswaldo Cruz, 2000; 95:367–373.
[18] Seema C C, Sharan S V, Srinivasa R B and Meena V . In vitro anti-inflammatory activity of Methanolic extract of Centella asiatica by HRBC Membrane stabilization. Rasayan journal of chemistry, 2011; 4 (2):457-460.
[19] Huang DJ, Lin CD, Chen HJ, Hou WC and Lin YH. Active recombinant thioredoxin h protein with antioxidant activities from sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage roots. J Agric Food Chem , 2004; 52:4720–4724.
[20] Pornpun Siramon and Yoshito Ohtani. Antioxidative and antiradical activities of Eucalyptus camaldulensis leaf oils from Thailand. J Wood Sci, 2007; 53:498–504.
[21] Chen CW, Ho CT. Antioxidant properties of polyphenols extracted from green and black tea. J Food Lipid, 1995; 2:35–46.
[22] Yen GC, Duh PD and Tsai CL. Relationship between antioxidant activity and maturity of peanut hulls. J Agric Food Chem, 1993; 41(1):67–70.
[23] Sudha G, Priya MS, Shree RI and Vadivukkarasi S. In vitro free radical scavenging activity of raw pepino fruit (Solanum muricatum). Int J Curr Pharm Res, 2011; 3(2):137–40.
[24] Aitadafouri M, Mounnnieri C, Heyman SF, Binistic C, Bon C and Godhold J .1996. 4-Alkoxybenzamides as new potent phospholipase A2 inhibitors. Biochem Pharm, 1996; 51:737–742.
[25] Ackerman NR and Beebe JB. Release of lysosomal enzymes by alveolar mononuclear cells. Nature, 1974; 247:475–477.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).