Synthesis, Characterization and Evaluation of Anticancer Activity of Nanoresveratrol in B16 Melanoma Cell Line
Resveratrol, a member of the stilbene family, is a naturally occurring polyphenolic compound in grapes and other botanicals. It carries a potential to prevent or treat several chronic diseases, including cancers. Despite various promising health benefits, including chemopreventive and chemotherapeutic benefits against cancer, clinical translation of resveratrol is hampered due to its poor bioavailability, solubility and stability. Here we present the study to prepare nano-formulation of resveratrol for improving the resveratrol profile. Calcium phosphate loaded nanoresveratrol has been synthesized, characterized and its anti-proliferative effects were examined in a B16 murine melanoma cell line. The anti-cancer effects of nanoresveratrol were directly compared with free resveratrol and chemotherapy drug 5-FU. Nanoresveratrol induced apoptosis in B16 melanoma more efficiently as compared to resveratrol alone and 5 FU. A nuclear staining profile following Hoechst staining of B16 cells is indicative of apoptosis and shows that the anti-cancer effect of nanoresveratrol is due to apoptosis induction. Since antioxidant activity is a hallmark of resveratrol, we also measured the biochemical antioxidant activities of superoxide dismutase (SOD) and catalase antioxidant enzymes. We found that, concurrent with the anti-cancer activity, the antioxidant activity of nanoresveratrol increased. Collectively, these findings clearly show that nanoresveratrol is superior to free resveratrol in suppressing cell growth in B16 murine melanoma cell line, and that the anti-cancer activity is primarily through apoptosis induction.
Keywords: Resveratrol, Nanoresveratrol, Formulation, B16 cell line, Cancer, Melanoma, Chemopreventive, Chemotherapeutic
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).