Method Development and Validation for Simultaneous Estimation of L-Glutathione and Vitamin-C in Effervescent Tablet by RP-HPLC
A simple, linear, precise, accurate and sensitive RP-HPLC method has been developed and validated for estimation of L-Glutathione and Vitamin-C in effervescent tablet. Isocratic elution at a flow rate of 1 ml/min tried on C18 column (ODS) 250 mm × 4.6 mm, 5µM on using a mobile phase consisting mixture of Acetonitrile:Methanol (6:4 v/v). The retention time of L-Glutathione was 7.208 minutes and Vitamin-C 3.825 minutes. The eluent was detected at 255 nm. Linearity was observed in the concentration range of 30-70μg/ml for L-Glutathione and 80-120μg/ml for Vitamin-C. The method is validated as per ICH guidelines. The proposed method can be successfully applied for estimation of L-Glutathione and Vitamin-C in effervescent tablet.
Keywords: L-Glutathione, Vitamin-C, RP-HPLC Method, Mobile phase, validation.
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