Combined Anticonvulsant Effect of Nifedipine and Pentazocine in Experimentally Induced Seizures by Maximal Electro Shock Method in Mice
Objective: The aim of the study was to determine the combined anticonvulsant effect of nifedipine (calcium channel blocker) and pentazocine (opioid analgesic) in experimentally induced seizures by Maximal Electro-Shock (MES) method in mice.
Methodology: The swiss albino mice weighing 20-40g of either sex were obtained from National Institute of Nutrition, Hyderabad after obtaining ethical approval. The pretreated mice are subjected to MES stimulation by electro convulsometer with alternate current at intensity required to produce tonic hind limb extension response. The animals showing positive response are divided into four groups (6 animals per group). Group I received distilled water, group II treated with nifidipine (10mg/kg/bw), group III pentazocine (30mg/kg/bw) and group IV combination of nifidipine (10mg/kg/bw) and pentazocine (30mg/kg/bw). Intraperitoneal is the route of administration. The parameters like duration of convulsions, Tonic Hind Limb Extension (THLE) and duration of recovery recorded. P<0.05 was considered as significant and P<0.001 was considered as highly significant.
Results: The duration of convulsions, duration of THLE and duration of recovery has been significantly reduced in combination treatment wih nifedipine 10mg/kg and pentazocine 30mg/kg compared to nifedipine 10mg/kg and pentazocine 30mg/kg individually.
Conclusion: The results obtained in this study provide supporting pharmacological evidence of efficacy, possible potential benefit of combining nifedipine with pentazocine in the treatment of epilepsy.
Keywords: Seizures, Maximal electroshock, Electroconvulsometer, anticonvulsant, Nifedipine, Pentazocine.
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