Formulation and Evaluation of Novel Herbal Formulations Incorporated with Amla Extract for Improved Stability
The objective of the present study was to formulate effervescent and fast dispersible granules by incorporating the fruit extract of Emblica officinalis as an alternate of liquid herbal juices available in market. Amla juice was extracted manually and then subjected to preliminary phytochemical screening which indicates the presence of alkaloids, glycosides, flavonoids, carbohydrates, phenolic compounds, proteins and phytosterols. Lyophilized amla powder was used to formulate effervescent and fast dispersible granules which were further optimized on the basis of concentration of superdisintegrants and effervescent producing agents like croscarmellose sodium, sodium starch glycolate, sodium bicarbonate and citric acid. Powdered formulations were then evaluated on basis of their flow properties like angle of repose, bulk density, tapped density, carr’s index, hausner’s ratio, effervescent cessation time and disintegration time. Among all the effervescent formulations F2 was found to be optimum as it was having least disintegration time of 22 seconds and showed excellent flow properties. In case of the fast dispersible formulations the optimum strength were shown by formulations F9 having croscarmellose with least disintegration time of 52 seconds. Total phenolic content of fresh amla juice were found to be 8.94 mg GAE/100 gm and estimation of ascorbic acid and gallic acid in lyophilized amla powder and developed formulations was carried out by HPTLC. In vitro antioxidant activity of lyophilized amla powder was evaluated by DPPH radical scavenging assay. IC50 value of lyophilized amla powder was found to be 32 ± 0.25 ug/ml calculated in comparison to standard ascorbic acid possessing IC50 value of 25.80 ± 0.2 ug/ml. Results of present study reveals that developed formulations may serve as alternate product with better quality, consistency and stability in comparison to available herbal liquid formulations.
Keywords: Anti-oxidant, Dispersible granules, Effervescent granules, Emblica officinalis.
2. Jain SK, Khurdiya D. Vitamin C enrichment of fruit juice based ready-to-serve beverages through blending of indian gooseberry (Emblica officinalis Gaertn.) Juice. Plant Foods for Human Nutrition, 2004;59, 63-66.3. Bajracharya MB. Ayurvedic Medicinal Plants and General Treatment, Piyusavarsi Ausadhalaya Kathmandu, Nepal. 1979; 230.
4. Thakur R, Puri HS, Husain A. Major medicinal plants of India. Lucknow: Central Institute of Medicinal and Aromatic Plants. 1989; 585.
5. Ghosal S, Tripathi VK, Chauhan S. Active constituents of Emblica officinalis: Part 1- The chemistry and antioxidant effects of two hydrolysable tannins, Emblicanin A and B. Indian Jour of Chemistry. 1996; 35B, 941–948.
6. Devalaraja S, Jain S, Yadav H. Exotic fruits as therapeutic complements for diabetes, obesity and metabolic syndrome. Food Res Inter, 2011; 44, 1856-1865.
7. Jeena KJ, Joy KL, Kuttan R. Effect of Phyllanthus Emblica Phyllanthus amarus and Picrorhiza kurroa on nitrosodiethylamine induced hepatocarcinogenesis. Cancer Lett,1999; 136:11–16.
8. Nireesha GR, Divya L, Sowmya C, Venkateshan N, Niranjan M, Lavakumar V. Lyophilization and freeze drying - An review, International Jour of Novel Trend in Pharm Sci. 2013; 3 (4) 87-98.
9. Kokate CK. Practical Pharmacognosy. 4thed. New Delhi: Vallabh Prakashan1994;pp.4.
10. Harborne JB. Phytochemical methods - A guide to modern technique of plant analysis, 3rded. UK: Champan and hall 1998; pp. 1-5.
11. Sandhya S, Gowthami G, Vinod KR, Sravanthi VE, Saikumar P, Rao KV, Banji D. Formulation and evaluation of herbal effervescent granules incorporated with Limnophila indica extract for bacillary dysentery, Ann of bio res, 2012; 3 (1):63-72.
12. Khalifa K, Ali M, Hassan SM, Abdalkreem M, Osman HM. Design formulation and evaluation of Senna effervescent tablets. Jour of For Pro & Ind, 2012; 1(2), 21- 25.
13 Lieberman HA, Lachman JB. Pharmaceutical dosage forms: tablets, Vol.1and 2, New york :Marcel dekker inc 1993; pp. 285.
14 Ansel HC, Allen V. Pharmaceutical dosage forms and Drug Delivery Systems, 6th ed. New Delhi: B. I. Waverly 1999; pp. 469-471.
15. James W. Pharmaceutical Preformulation: The physicochemical properties of drug substances: In: Aulton ME. Pharmaceutics the science of dosage form design, 2nd edition, Spain: Churchill Livingstone 2006; pp. 113-138.
16 Peter D, Gibson M. Oral solid dosage forms: Pharmaceutical preformulation and formulation. A practical guide from candidate drug selection to c ommercial dosage form, 2nd ed. New York: CRC Press 2008; pp.379-432.
17. Kulkarni U. Design and development of fast disintegrating tablets containing Amla powder by vacuum drying technique. Inter Jour of Pharm Res, 2001; 3, 2124-2134.
18.Kulkarni U, Manthale D, Patil BS. Design and development of fast disintegrating tablets containing Ashwagandha by sublimation technique. Inter Jour of Pharm Res, 2011; 64, 82-94.
19. Shanaz B, Arunachalam G, Jayaveera KN, Ashoka VL, Premakumari KB. Estimation of total phenolic content and in vitro antioxidant activity of Barleria Montana. Der Pharm Lett, 2011; 3 (4):178-182
20. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac Jour Trop Biomed. 2013; 3(8): 623–627.
21. Archana D, Rajasekaran A, Arivukkarasu R. HPTLC Method for estimation of Gallic acid and Rutin in Haritak An Ayurvedic Formulation. Inter Jour of Pharm Tech Res.2011; 3(2), 986-999.
22. Leela V, Saraswathy A. Quantification of Pharmacologically active markers Gallic acid, quercetin and lupeol from Acacia leucophloea wild flowers by HPTLC method . International Res Jour of Pharm. 2013; 4(2):146-150
23 Sajeeth CI, Manna PK, Manavalan R, Jolly CI. Quantitative estimation of gallic acid, rutin and quercetin in certain herbal plants by hptlc method. Der Chem Sin. 2010; 1 (2): 80-85
24. Jamuna, S, Paulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Jour of Appl Pharm Sci 2012; 2 (07):149-154
25. Baldi A, Hussain W, Tailor Y. Evaluation of in-vitro cultured cells of Withania somnifera for antioxidant activity. Curr trend in Biotech and Pharm, 2010; 4, 589-595.
26. Baldi A, Choudhary N. In vitro antioxidant and hepatoprotective potential of Chenopodium album extract. International Journal of Green Pharmacy, 2013; 7(1), 50-56.
27. Baldi A, Evaluation of in vitro antioxidant activity of Eclipta alba. Acta Horticulturae. 972, International Society for Horticultural Science 2013; pp.27-30.
28. Baldi A, Pandit MK, Ranka P. Amelioration of in-vivo Antioxidant Activity by Banana Extracts. International Journal of Pharmaceutical & Biological Archive, 2012;3(1), 157-161.
29. Baldi A, Hussain W, Tailor Y. Evaluation of in-vitro Cultured Cells of Withania somnifera for Antioxidant Activity. Current Trends in Biotechnology and Pharmacy, 2010; 4, 589- 595.
30. Singh PA, Brindavanam NB, Kimothi GP, Aeri V. Evaluation of in vivo anti-inflammatory and analgesic activity of Dillenia indica f. elongata (Miq.) Miq. and Shorea robusta stem bark extracts. Asian Pacific Journal of Tropical Disease, 2016; 6(1), 75-81.
31. Singh PA, Brindavanam NB, Kimothi GP, Verma R, Aeri V. A validated HPLC method for the determination of betulin in the stem bark of Tectona grandis Linn. International Journal of Pharmaceutical Sciences and Research, 2016; 7(2)719-723.
32. Singh PA, Vidhu A. Physico-chemical parameters and HPTLC fingerprinting profile of Dillenia indica Miq. f. Elongata (Miq.) and Tectona grandis Linn. with reference to betulin. International Journal of pharma and bio sciences, 2016;7(1)217-221
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