Development and Characterization of Morin Loaded Phytosomes for its Anti-Oxidant Activity
The herbal plants contain huge number of active constituents, which shows excellent biological activity in-vitro but less in-vivo because of their poor lipid solubility, improper molecules size, degradation in gastric fluid and destruction of active components in gut by bacteria. So that poor absorption and bioavailability may occur. Phytosomal technique can be applied to herbal actives for the enhancement of bioavailability of herbal extracts and phytoconstituents. Phytosomes are prepared by patented process where standardized plant extract are bound to phospholipid. The aim of the present study was to developed and characterized the phytosomes of Morin to overcome the limitation of absorption and bioavailability. Phytosomes of Morin was formulated by thin film hydration method by using in different ratio of lipid with phytoconstituents. The prepared phytosomes were characterized for their drug content, drug entrapment, particle size, in-vitro release study and evaluated its in vitro antioxidant activity. From the present study, the result showed that spherical shape phytosomes having particle size 661.2nm. The poly-dispersity index and zeta potential was found to be 0.383 and -13.76 mv respectively. Result obtained from in-vitro release studies shows a sustained release of morin from formulation over a period of 12 hrs. From the present study it was concluded that morin loaded phytosmes shows better absorption and more effective antioxidants than the parent compound.
Keyword: Phytosomes, phospholipids, Morin, in-vitro Antioxidant activity
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