Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities
Abstract
Aim: The present study is aimed to evaluate in-vitro anti-inflammatory and anti-bacterial activity of phenolic ethereal oils like Tulsi (Ocimum tenuiflorum) and Clove (Syzygium aromaticum).
Materials and methods: A total of 500 g of fresh leaves and dried flower buds of Tulsi and Clove were subjected to hydro-distillation method for 6 h using Clevenger’s apparatus. The isolated ethereal oils were used for testing the in-vitro anti-inflammatory activity by using albumin denaturation assay, proteinase inhibitory activity at a concentration of 20, 40, 60, 80 and 100 µl/ml and anti-bacterial activity against two gram positive microorganisms (Bacillus subtilis and Staphylococcus aureus) and two gram negative microorganisms (Salmonella typhi and Escherichia coli) at concentrations 50 µl/ml, 100 µl/ml and 200 µl/ml by adopting cup plate method.
Results: The isolated ethereal oils exhibited significant in-vitro anti-inflammatory effect and also inhibited the growth of both Gram positive and Gram negative microorganisms at 50 µl/ml, 100 µl/ml and 200 µl/ml concentrations.
Conclusion: The findings of this study showed that the effectiveness of Phenolic ethereal oils isolated from Clove and Tulsi. Clove (Syzygium aromaticum) showed enhanced anti-inflammatory and anti-bacterial activity compared to Tulsi (Ocimum tenuiflorum). The present study provides evidence that Ocimum tenuiflorum and Syzygium aromaticum; Phenolic ethereal oils contain medicinally important bioactive components justifying its traditional use.
Keywords: Phenolic ethereal oils, anti-inflammatory activity, anti-bacterial activity, Ociumm tenuiflorum, Syzygium aromaticum.
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References
2. Mohamed E, Ibrahim M, Hend E. Introduction of Ocimum tenuiflorum plant to the Egyptan Cultivation. International Journal of PharmaTeach Research 2016; 9:18-24.
3. Joshi RK, Hoti SL. Chemical composition of the essential oil of Ocimum tenuiflorum L. from North West Karnataka, India. Plant Science Today 2014; 1:99-102.
4. Diego F, Cortes R, Claudia R, Fernandes S. Clove: a precious spice. Asian pac J Trop Biomed 2014; 4:90-6.
5. Dagli N, Dagli R, Mahmoud RS, Baroudi K. Essential oils their therapeutic properties, and implication in dentistry: A review. J Int Soc Prev Community Dent 2015; 5:335-40.
6. Muchalal M, Crouzet J. Volatile components of clove essential oil (Eugenia caryophyllus spreng) Neutral fraction. Agric Biol Chem 1985; 49:1583-9.
7. Sangita C, Priyanka C, Protapaditya D, Sanjib B. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine 2012; 178-80.
8. Oyedepo O, Femurewa AJ. Anti‐protease and membrane stabilizing activities of extracts of Fagrazanthoxiloides, Olaxsubscorpioides and Tetrapleura tetraptera. Int J of Pharmacong 1995; 33:65‐9.
9. Nyiligira E, Viljoen VM, Vuuren SF. Essential oil composition and in vitro antimicrobial and anti-inflammatory activity of South African Vitex species. South African Journal of Botany 2004; 70:611–7.
10. Ashraf A, Mostafa A, Khalid S, Turki M. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences 2018; 25:361-6.
11. Narender Boggula. Phytochemical evaluation and in vitro anti bacterial activity of dried seeds of Abrus precatorius. International journal of pharmaceutical sciences review and research 2017; 44(1):101-7.
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