Wound healing prospective of pongamia glabra, piper nigrum and momordica charantia on albino rats using anemic burn wound model
Abstract
Objective: The objective of present work was to evaluate wound healing potential of pongamia glabra, piper nigrum and momordica charantia using as herbal ointment formulation on albino rats using burn wound model. All the plant has the rich source of alkaloid, glycoside, tannins, carbohydrates, saponins, flavonoids, proteins and amino acids content and were used as anti-diabetic, anti-inflammatory, antitumor, anti-malarial and having wound healing potential. Materials and Methods: Extract of all three plants has been separated by the Soxhlet extraction. Herbal ointment formulation has been prepared by mixing the extract of momordica charantia, pongamia glabra and piper nigrum with the wool fat and paraffin. Burn wound model has been utilized for the evaluation of wound healing potential. Histopathological evaluation has been also carried-out for the physical verification wound healing potential. Results and Conclusion: The studies on burn wound healing model reveals that all twelve groups showed decreased wound area on the time and there was no mortality observed in the course of study. Discussion: These studies have indicated that herbal ointment formulation of pongamia glabra, piper nigrum and momordica charantia has been utilized for wound healing and it is safer for topical application. No toxicity and mortality have been observed during the experimental tenure.
Keywords: Wound healing, momordica charantia, pongamia glabra, piper nigrum, burn wound model
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2. Sharma MC, Joshi C. Plants used in skin diseases of animal. Natural product radiance. 2004; 3(4):293-299.
3. Krishnan SK, Pavan KG. Evaluation of antimicrobial activity of roots of Pongamia glabra vent. International Journal of Pharmaceutical and Chemical Sciences. 2013; 2(1):303-304.
4. Sunila ES, Kuttan G. Immunomodulatory and antitumor activity of Piper longum Linn. and piperine. Journal of Ethnopharmacology. 2004; 90(2-3):339-346.
5. Jayasooriva AP, Sakono P. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets, Journal of ethanopharmacology.2000; 72(1-2):331-336.
6. Chia YY, Yap Wai-Sum. In-vitro Antimicrobial activity of hexane: petroleum ether extracts from fruits of Momordica charantia L. International Journal of Pharmaceutical and Biological Archives. 2011; 2(3):868-873.
7. Rawat P, Gill NS, Arora R. Study of momordica charantia seeds for their antioxidant Potential. International Journal of Natural Product Science. 2012; 1:223-227.
8. Khan AW, Kotta S, Ansari SH, Sharma RK, Kumar A, Ali J. Formulation development, optimization and evaluation of aloe vera gel for wound healing. Pharmacognosy Magzine. 2013; 1:S6-S10.
9. Sawant SE, Tajane MD. Formulation and evaluation of herbal ointment containing Neem and Turmeric extract. Journal of Scientific and Innovative Research. 2016; 5(4):149-151.
10. Walker HL, Mason AD. A standard animal burn. Journal of Trauma - Injury, Infection and Critical Care. 1968; 8(6):1049–1051.
11. Dorsett-Martin WA. Rat models of skin wound healing: a review. Wound Repair and Regeneration. 2004; 12(6):591–599, 2004.
12. Ferreira LM, Hochman B, Barbosa MVJ. Experimental models in research. Acta Cirurgica Brasileira. 2005; 20(2):28-34.
13. Venter NG, Monte-Alto-Costa A, Marques RG. A new model for the standardization of experimental burn wounds. Burns 2015; 41(3):542–547.
14. Porumb V, Trandabăț AF, Terinte C, Căruntu ID, Porumb-Andrese E, Dimofte MG. Pieptu D. Design and Testing of an Experimental Steam-Induced Burn Model in Rats. BioMed Research International. 2017; 2(2):710-715.
15. Nakae H, Inaba H. Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model. Journal of Trauma. 2000; 49(3):511-514.
16. Pawar RS, Chaurasiya PK, Rajak H, Singour PK, Toppo FA, Jain A. Wound healing activity of Sida cordifolia Linn. in rats. Indian Journal of Pharmacology. 2013; 45:474-8
17. Guo HF, Ali RM, Hamid RA, Zaini AA, Khaza'ai H. A new model for studying deep partial-thickness burns in rats. International Journal of Burns and Trauma. 2017; 7(6):107-114.
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