Isolation and characterization of bioactive compound anthraquinone from methanolic extract of Boerhavia diffusa linn.

  • U Kanagavalli Assistant Professor, Department of Biochemistry, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India
  • A. Mohamed Sadiq Principal, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India

Abstract

Boerhavia diffusa Linn. (Nyctaginaceae), commonly known as ‘Punarnava’ is a perennial creeping herb widely studied and has a long history of uses by the tribal people and in Ayurvedic and Unani medicines. Our previous study showed that in different extracts of Boerhavia diffusa (ethanol, methanol, petroleum ether, aqueous and hexane), methanolic extract had significant anti-oxidant activity, but the active components present in that extracts are still unclear. In this study, Boerhavia diffusa Linn was investigated for the bioactive compounds present in its methanolic extract. Separation and purification of the compounds in the most active methanol extract was done using a combination of column chromatography and thin layer chromatography. The compound was identified using gas chromatography and mass spectrophotometry. The resulted compound anthraquinone which is also called as 9, 10 anthracenedione. In addition to their known use as natural dyes, it have several biological activities like antitumor, antiinflammatory,  diuretic , antiarthritic, antifungal, antibacterial,  antimalarial and antioxidant activities . This justifies the use of this plant in traditional medicine and indicates a promising potential for the development of medicinal agents from Boerhavia diffusa.


Keywords: Antioxidant, Boerhavia diffusa Linn, anthraquinone, column chromatography, Thin layer Chromatograhy, GC-MS.

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Author Biographies

U Kanagavalli, Assistant Professor, Department of Biochemistry, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India

Assistant Professor, Department of Biochemistry, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India

A. Mohamed Sadiq, Principal, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India

Principal, Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, Vellore District, Tamilnadu, India

References

1. Sayed A etal.,Anthraquinone glycosides from Cassia roxburghii and evaluation of its free radical scavenging activity., Carbohydrate Research 2012; 360:47–51.
2. Nagy Morsy, Phytochemical analysis of biologically active constituents of medicinal plants Main Group Chemistry 2014; 13:7–21.
3. Sahu.A. Phytopharmacological Review of Boerhaavia diffusa Linn.(Punarnava) Pharmacognosy Reviews, Supplement 2008; 2(4).
4. Gaspar Diaz-Munoz etal., Anthraquinones: An Overview., Studies in Natural Products Chemistry, 313–338.
5. Vivek K. etal., Isolation and purification of plant secondary metabolites using column-chromatographic technique, Bangladesh J Pharmacol. 2016; 11:844-848.
6. Kanthal LK., etal., GC-MS analysis of bio-active compounds in methanolic extract of Lactuca runcinata, Pharmacognosy Research, 2014; 6(1):58-61.
7. Altemimi, A.W etal., Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a tlc-densitometric method. Chem. Cent. J. 2015; 9:1–15.
8. Altemimi, A et al., Employing response surface methodology for the optimization of ultrasound assisted extraction of lutein and β-carotene from spinach. Molecules 2015, 20,
6611–6625.
9. Mulinacci, N etal., Commercial and laboratory extracts from artichoke leaves: Estimation of caffeoyl esters and flavonoidic compounds content. J. Pharm. Biomed. Anal. 2004; 34:349–357.
10. Sarajlija,H etal., Preparation of flaxseed for lignan determination by gas chromatography-mass spectrometry method. Czech J. Food Sci. 2012; 30:45–52.
11. Zhang, Z etal., Role of peroxidase in anthocyanin degradation in litchi fruit pericarp. Food Chem. 2005; 90:47–52.
12. Popova, I.E etal., Determination of lignans in flaxseed using liquid chromatography with time-of-flight mass spectrometry. J. Chromatogr. A 2009; 1216:217–229.
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1.
Kanagavalli U, Sadiq AM. Isolation and characterization of bioactive compound anthraquinone from methanolic extract of Boerhavia diffusa linn. JDDT [Internet]. 1Oct.2018 [cited 29Mar.2024];8(5-s):332-7. Available from: https://www.jddtonline.info/index.php/jddt/article/view/1987
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