FORMULATION AND EVALUATION OF TROPICAMIDE IN-SITU GELS LOADED SOLID LIPID NANOPARTICLES FOR OCULAR DRUG DELIVERY
The aim of present work Formulation and Evaluation of Tropicamide In-situ Gels loaded Solid Lipid Nanoparticles for Ocular Drug Delivery. The surface morphological of SLN was carried out by TEM. The Tropicamide loaded solid lipid nanoparticles was measured the average particle size was ranges from 182.1+3.12nm to 390.1Â±2.10 nm. The zeta potential ranges from -0.17Â±1.4 mV to -3.80Â±1.5 mV. The entrapment efficiency 66.2 % to 89.2 %. Drug content was ranges from 0.112mg/ml to 0.502 mg/ml. The percentage yield ranges from was ranges from 0.112mg/ml to 0.502 mg/ml. The polydispersity index ranged from 1.011Â±0.15 to 1.327Â±0.13. These SLN enriched in Chitosan gels the pH of the formulations range from 6.8 to 6.9. The gelling strength ranged from 129 sec to 152 sec. The bioadhesive force was ranges from 10.21 Â±1.15 dynes/cm2 to 15.23 Â± 1.22 dynes/cm2. The viscosity was ranges from 2212 Â± 1.14 cps to 2420Â± 1.19 cps. The spreadability coefficient was ranges from 11.2 Â± 1.10 gms/sec to 13.3 Â± 1.21 gms/sec. The in-vitro diffusion release studies carried out at 12 hrs TSLNGF19 shows the 79.2 Â± 0.32. The ex vivo permeation studies for optimized formulation the increased drug permeation and corneal accumulation. In vitro corneal permeation profile of tropicamide loaded SLN from the chitosan gels and commercial eye drop solution (Tropicacyl) across the isolated porcine cornea. The ocular tolerance studies performed with HETCAM assay, corneal hydration study, histopathological studies. The stability studies of chitosan gels for long-term stability as per ICH guidelines (25Â°C Â± 2Â°C / 60% RH Â± 5% RH) &accelerated stability as per ICH guidelines (40Â°C Â± 2Â°C / 75% RH Â± 5% RH) there is no changes in gelling strength, bioadhesive force, viscosity, spreadability coefficient in optimized formulation.
Keywords: Chitosan, Corneal hydration studies, ex vivo permeation, in vitro diffusion studies, Solid Lipid Nanoparticles
2. Sharma UK, Verma A, Kumar S, Pandey H, Pandey A. In vitro, in vivo and pharmacokinetic assessment of amikacin sulphate laden polymeric nanoparticles meant for controlled ocular drug delivery, Applied Nanoscience, 2015; 5:143-152.
3. Campose A, Diebold Y, Alonso MJ. Chitosan nanoparticles as new ocular drug delivery systems: in vitro stability, in vivo fate, and cellular toxicity, Journal of Pharmaceutical Research 2004; 21(5): 807-9.
4. Tripathi KD. Anticholinergic drugs and drugs acting on autonomic ganglia, Essential of Medical Pharmacology, Published by Jaypee Brothers Medical Publishers (P) Ltd. 2008; 6: 110-111.
5. Mudgil M, Gupta N, Nagpal M, Pawar P. Nanotechnology: A new approach for ocular drug delivery, International Journal of Pharmacy and Pharmaceutical Sciences, 2012; 4(2):105-10.
6. Fathalla ZM, Khaled AK, Hussein AK, Alany RG, Vangala A. Formulation and corneal permeation of ketorolac tromethamine loaded chitosan nanoparticles. Journal of Drug Development and Industrial Pharmacy 2015; 1:2-9.
7. Cavalli, R., Caputo, O., Gasco, M.R., Preparation and characterization of solid lipid nanospheres containing paclitaxel. Eur. J. Pharm. Sci. 2000; 10, 305-309.
8. Tiyaboonchai, W., Tungpradit, W., Plianbangchang, P., 2007. Formulation and characterization of curcuminoids loaded solid lipid nanoparticles. Int. J. Pharm. 2007; 337, 299-306.
9. Freitas, C., Muller, R.H., 1998. Effect of light and temperature on zeta potential and physical stability in solid lipid nanoparticle dispersions. Int. J. Pharm. 168, 221-229.
10. Hippalgaonkar, K., Adelli, G.R., Hippalgaonkar, K., Repka, M.A., Majumdar, S., Indomethacin-loaded solid lipid nanoparticles for ocular delivery: development, characterization, and in vitro evaluation. J. Ocul. Pharmacol. Ther. 2013, 29, 216-228.
11. Brahamdutt, Manjusha Choudhary, Sandeep Kumar, Mohit Bhatia, Vikaas Budhwar. Formulation and in-vitro evaluation of sustained release tropicamide loaded chitosan nanoparticles for ocular drug delivery. Int. Res. J. Pharm. 2016;7(10):27-35
12. Cavalli, R., Gasco, M.R., Chetoni, P., Burgalassi, S., Saettone, M.F., 2002. Solid lipid nanoparticles as ocular delivery system for tobramycin. Int. J. Pharm. 2002; 238, 241245.
13. AcevedoMorantes, C.Y., AcevedoMorantes, M.T., SuleimanRosado, D., RamÃrezVick, J.E., Evaluation of the cytotoxic effect of camptothecin solid lipid nanoparticles on MCF7 cells. Drug Deliv. 2013; 20, 338-348.
14. Attama, A.A., Reichl, S., Muller-Goymann, C.C., Diclofenac sodium delivery to the eye: in vitro evaluation of novel solid lipid nanoparticle formulation using human cornea construct. Int. J. Pharm. 2008; 355, 307-313.
15. Jayachandra Reddy Peddappi Reddigari, Yerikala Ramesh et al. Chandrasekhar B.Kothapalli, Formulation and Evaluation of in situ gels enriched with tropicamide loaded solid lipid nanoparticles, International Journal of Research in Pharmaceutical sciences, 2018, 9(1), 216-225.
16. Balls, M., Botham, P.A., Bruner, L.H., Spielmann, H., 1995. The EC/HO international validation study on alternatives to the Draize eye irritation test. Toxicol. In Vitro, 1995, 9, 871929.
17. Zur-Muhlen, A., Schwarz, C., Mehnert, W., Solid lipid nanoparticles for controlled drug delivery-drug release and release mechanism. Eur. J. Pharm. Biopharm. 1998, 45, 149-155.
18. Basaran, E., Demirel, M., Sirmagul, B., Yazan, Y., Cyclosporine-An incorporated cationic solid lipid nanoparticles for ocular delivery. J. Microencapsul. 2010, 27, 37-47.
19. Zhu, H., Chauhan, A., Effect of viscosity on tear drainage and ocular residence time. Optom. Vis. Sci. 2008, 85, E715-E725.
20. Li, J., Guo, X., Liu, Z., Okeke, C.I., Li, N., Zhao, H., Aggrey, M.O., Pan, W., Wu, T., Preparation and evaluation of charged solid lipid nanoparticles of tetrandrine for ocular drug delivery system: pharmacokinetics, cytotoxicity and cellular uptake studies. Drug Dev. Ind. Pharm. 2014, 40, 980-987.
21. seyfoddin, A., Shaw, J., Al-Kassas, R., Solid lipid nanoparticles for ocular drug delivery. Drug Deliv. 2010, 17, 467-489.
22. Zhang, X., Lu, S., Han, J., Sun, S., Wang, L., Li, Y., Preparation, characterization and in vivo distribution of solid lipid nanoparticles loaded with syringe picroside. Pharmazie, 2011, 66, 404-407.
23. Purnima Verma & Munish Ahuja, 2016 Cubic liquid crystalline nanoparticles: optimization and evaluation for ocular delivery of tropicamide, Drug Delivery, 2016, 23:8, 3043-3054.
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