FORMULATION AND EVALUATION OF TROPICAMIDE IN-SITU GELS LOADED SOLID LIPID NANOPARTICLES FOR OCULAR DRUG DELIVERY

  • Yerikala Ramesh Research Scholar, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India
  • Jayachandra Reddy Peddappi Reddigari Principal & Professor, Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Chadalawada Nagar, Renigunta Road, Tirupathi - 517506, Andhra Pradesh, India.
  • Chandrasekhar B. Kothapalli Director Foreign Affairs and Alumni Matters, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India

Abstract

The aim of present work Formulation and Evaluation of Tropicamide In-situ Gels loaded Solid Lipid Nanoparticles for Ocular Drug Delivery. The surface morphological of SLN was carried out by TEM. The Tropicamide loaded solid lipid nanoparticles was measured the average particle size was ranges from 182.1+3.12nm to 390.1±2.10 nm. The zeta potential ranges from -0.17±1.4 mV to -3.80±1.5 mV. The entrapment efficiency 66.2 % to 89.2 %. Drug content was ranges from 0.112mg/ml to 0.502 mg/ml. The percentage yield ranges from was ranges from 0.112mg/ml to 0.502 mg/ml. The polydispersity index ranged from 1.011±0.15 to 1.327±0.13. These SLN enriched in Chitosan gels the pH of the formulations range from 6.8 to 6.9. The gelling strength ranged from 129 sec to 152 sec. The bioadhesive force was ranges from 10.21 ±1.15 dynes/cm2 to 15.23 ± 1.22 dynes/cm2. The viscosity was ranges from 2212 ± 1.14 cps to 2420± 1.19 cps. The spreadability coefficient was ranges from 11.2 ± 1.10 gms/sec to 13.3 ± 1.21 gms/sec. The in-vitro diffusion release studies carried out at 12 hrs TSLNGF19 shows the 79.2 ± 0.32. The ex vivo permeation studies for optimized formulation the increased drug permeation and corneal accumulation. In vitro corneal permeation profile of tropicamide loaded SLN from the chitosan gels and commercial eye drop solution (Tropicacyl) across the isolated porcine cornea. The ocular tolerance studies performed with HETCAM assay, corneal hydration study, histopathological studies. The stability studies of chitosan gels for long-term stability as per ICH guidelines (25°C ± 2°C / 60% RH ± 5% RH) &accelerated stability as per ICH guidelines (40°C ± 2°C / 75% RH ± 5% RH) there is no changes in gelling strength, bioadhesive force, viscosity, spreadability coefficient in optimized formulation.

Keywords: Chitosan, Corneal hydration studies, ex vivo permeation, in vitro diffusion studies, Solid Lipid Nanoparticles

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Author Biographies

Yerikala Ramesh, Research Scholar, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India

Research Scholar, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India

Jayachandra Reddy Peddappi Reddigari, Principal & Professor, Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Chadalawada Nagar, Renigunta Road, Tirupathi - 517506, Andhra Pradesh, India.

Principal & Professor, Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Chadalawada Nagar, Renigunta Road, Tirupathi - 517506, Andhra Pradesh, India.

Chandrasekhar B. Kothapalli, Director Foreign Affairs and Alumni Matters, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India

Director Foreign Affairs and Alumni Matters, Jawaharlal Nehru Technological University Anantapur, Ananthapuramu - 515002, Andhra Pradesh, India

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Ramesh Y, Reddigari J, Kothapalli CB. FORMULATION AND EVALUATION OF TROPICAMIDE IN-SITU GELS LOADED SOLID LIPID NANOPARTICLES FOR OCULAR DRUG DELIVERY. JDDT [Internet]. 18Jun.2018 [cited 31Oct.2020];8(2):194-07. Available from: http://www.jddtonline.info/index.php/jddt/article/view/1765