HYPOGLYCEMIC EFFECT OF POLYHERBAL FORMULATIONS IN ALLOXAN INDUCED DIABETIC MICE
Objective: The present work was executed to evaluate the antidiabetic potency of herbal preparation which was the combination of wheat germ oil, fresh juice of CorianderÂ sativumÂ and Aloe vera gel in normal andÂ alloxan-inducedÂ diabetic mice.
Methods: TheÂ serum glucose lowering activity of the herbal preparation (PH-1 in the ratio of 1:1:1of wheat germ oil, fresh juice of C.sativum and Aloe vera) was studied in normal rats after oral administration at 1.0 and 2.0ml/kg and herbal preparation (PH-1, PH-2 wheat germ oil, fresh juice of coriander sativum and Aloe vera gel in ratio of 2:2:1) and PH-3 (wheat germ oil, fresh juice of coriander sativum and Aloe vera gel in ratio of 1:2:2) on alloxan induced diabetic swiss albino mice, at doses of 1.0 and 2.0 ml/kg. Blood samples were collected and analyzed for serum glucose at 0,1/2, 1, 2, 4, 8, 12 and 24 h in normal mice and at 0, 1, 3, 7, 15 and 30 days in diabetic mice after drug administration by glucose oxidase/peroxidase method. The data were compared statistically by using one way ANOVA method followed by Dunnett multiple componentÂ test. Statical significance was set at p<0.05.
Results: TheÂ herbal preparation (1.0 and 2.0 ml/kg) produced significant (p<0.05) reduction in serum glucose level of both normal and diabetic mice and comparable with that of control and standard drug Glibenclamide.
Conclusion: TheÂ herbal preparation showed significant glucose lowering effect in normal and alloxan induced diabetic rats. This preparation is giving to be a promising antidiabetic preparation for masses and however, it requires further extensive studies in human beings.
Keywords: Antidiabetic potency, alloxan-induced diabetic rats, Aloe vera
2. Kaveeshwar SA, Cornwall J. The current state of diabetes mellitus in India. Australas Med J. 2014; 7(1):45â€“48.
3. Ryan G, Briscoe TA, Lynette L. Review of pramlintide as adjunctive therapy in treatment of type 1 and type 2 diabetes. Drug Des Devel Ther. 2009; 2:203â€“214.
4. World Health Organization, â€œsecond report of the WHO Expert committee on diabetes mellitusâ€, Geneva, Technical Report Series; 1980; 646-666.
5. Swarnkar R, Kaushal C, Jain SK, Hypoglycaemic activity of Centratherum anthelminticu in experimental animals, Journal of Drug Delivery and Therapeutics. 2017; 7(4):73-77 DOI: http://dx.doi.org/10.22270/jddt.v7i4.1472
6. Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989; 12:553-564.
7. Dangre PV, Fating VD, Wakodkar SB, Godbole MD, Development of stevioside loaded pellets for regulating the blood glucose level in diabetes: in vivo- in vitro characterization, Journal of Drug Delivery and Therapeutics. 2017; 7(4):59-65 DOI: http://dx.doi.org/10.22270/jddt.v7i4.1461
8. L. Verma, A. Khatri, B. Kaushik, U.K. Patil, R.S. Pawar, â€œAntidiabetic activity of Cassia occidentalis (Linn) in normal and alloxan-induced diabetic rats,â€ Indian Journal of Pharmacology, 2010; 42(4):224-228.
9. Manivannan R, Shopna R, Antidiabetic activity of calotropis gigantea white flower extracts in alloxan induced diabetic rats. Journal of Drug Delivery and Therapeutics, 2017; 7(3):106-111. doi:10.22270/jddt.v7i3.1447
10. Gurjar H, Pandey H, Verma A, Irchhaiya R, Singh P, Antidiabetic activity of bauhinia variegata extracts in alloxan-induced diabetic rats. Journal of Drug Delivery and Therapeutics, 2018; 8(1):29-32. doi:10.22270/jddt.v8i1.1548
11. Issa IA, Bule H, â€œHypoglycemic Effect of Aqueous and Methanolic Extract of Artemisia afra on Alloxan Induced Diabetic Swiss Albino Mice,â€ Evidence-Based Complementary and Alternative Medicine, pp. 5, 2015.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).