GC-MS PROFILING, CHEMICAL CHARACTERIZATION, ANTIOXIDANT, Α-AMYLASE AND Α-GLUCOSIDASE INHIBITION OF SELECTED SEAWEEDS FROM SOUTHEAST COAST OF INDIA: AN IN VITRO APPROACH

  • Paramasivam Deepak Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India
  • Rajamani Sowmiya Greensmed labs, Thoraipakkam, Chennai-600 097, India
  • Chinnaperumal Kamaraj Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India
  • Muthu Paulraj Diviya Josebin Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India
  • Dilipkumar Aiswarya Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India
  • Govindasamy Balasubramani Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India
  • Vadivel Amutha Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India
  • Pachiappan Perumal Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Abstract

The present study focuses on in vitro antioxidant and enzyme inhibitory activity of three different solvent extracts (Methanol, Ethyl acetate and Hexane) of 3 different seaweeds viz: Sargassum wightii, Caulerpa racemosa, and Acanthophora spicifera. The preliminary phytochemical analyses of the seaweed extracts have revealed the presence of various phytochemicals. The antioxidant activities of the seaweed extracts have shown the scavenging effect. Among the extracts, SWEA, SWME and SWHE have exerted effective antioxidant activity with the IC50 values (µg/mL) of: 32.86, 130.1 and 212.8, respectively. And similar trend of α-amylase and α-glucosidase activity/inhibitory property by seaweeds have been recorded. Hence, the ethyl acetate extract of S. wightii was subjected to gas chromatography. All the seaweed extracts were characterized through HPLC and, FTIR analyses. The GC-MS analysis of ethyl acetate extract of S. wightii showed the presence of a bioactive compound, Heptadecanoic acid that might be have been the reason for the recorded inhibitory activity.

Keywords: Seaweeds, Sargassum wightii, DPPH, Column chromatography, GC-MS.

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Author Biographies

Paramasivam Deepak, Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Rajamani Sowmiya, Greensmed labs, Thoraipakkam, Chennai-600 097, India

Greensmed labs, Thoraipakkam, Chennai-600 097, India

Chinnaperumal Kamaraj, Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Muthu Paulraj Diviya Josebin, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Dilipkumar Aiswarya, Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Department of Biotechnology, School of Biosciences, Periyar University, Salem – 636 011, India

Govindasamy Balasubramani, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Vadivel Amutha, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Pachiappan Perumal, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore-641046, India

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Deepak P, Sowmiya R, Kamaraj C, Josebin MPD, Aiswarya D, Balasubramani G, Amutha V, Perumal P. GC-MS PROFILING, CHEMICAL CHARACTERIZATION, ANTIOXIDANT, Α-AMYLASE AND Α-GLUCOSIDASE INHIBITION OF SELECTED SEAWEEDS FROM SOUTHEAST COAST OF INDIA: AN IN VITRO APPROACH. JDDT [Internet]. 14Mar.2018 [cited 31Oct.2020];8(2):60-2. Available from: http://www.jddtonline.info/index.php/jddt/article/view/1665