REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE QUNTIFICATION OF CEFOTAXIME SODIUM IN PHARMACEUTICAL DOSAGE FORM
A simple, sensitive, fast & precise Reverse Phase High Performance Liquid Chromatographic method was developed for the determination of Cefotaxime Sodium in pharmaceutical dosage form. The RP-HPLC separation was achieved on hypersil C18 column (250 mm, 4.6 mm, 5Âµm) using mobile phase consisting ofÂ buffer solution (sodium dihydrogen phosphate) : acetonitrile Â [37: 63 v/v, pH=2.75 adjusted with phosphoric acid] at a flow rate of 1ml/min and the retention time was about 6 minutes. The method is selective to Cefotaxime Sodium and able to resolve the drug peak from formulation excipients. The system suitability with retention time was (Mean + %CV) 8.600 + 0.186.The calibration curve was linear over the concentration range of 1-20Âµg/ml (r2 = 0.999). The proposed method is accurate and precise (Intra day and Inter day variation, RSD were 0.55-1.67) and linear within the desired range. The LOD and LOQ was detected as 0.0187Âµg/ml and 0.043Âµg/ml respectively with r2 = 0.9997. The accuracy result of seventy percent drug (80%) was 99.87%, hundred percent (100%) was 99.93%, and one thirty percent (120%) was 100.18%. Therefore, this method could be used as a more convenient and efficient option for the analysis of Cefotaxime Sodium in raw material and Parentral dosage form.
Keywords:Â Â Â ThirdÂ Â Â generationÂ Â Â cephalosporin;Â Â Â Cefotaxime Sodium;Â Â Â MethodÂ Â Â validation;Â Â Â HPLCÂ Â Â method determination; Quantitative analysis
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