REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE QUNTIFICATION OF CEFOTAXIME SODIUM IN PHARMACEUTICAL DOSAGE FORM

  • RY Shah Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India
  • RK Jat Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

Abstract

A simple, sensitive, fast & precise Reverse Phase High Performance Liquid Chromatographic method was developed for the determination of Cefotaxime Sodium in pharmaceutical dosage form. The RP-HPLC separation was achieved on hypersil C18 column (250 mm, 4.6 mm, 5µm) using mobile phase consisting of  buffer solution (sodium dihydrogen phosphate) : acetonitrile  [37: 63 v/v, pH=2.75 adjusted with phosphoric acid] at a flow rate of 1ml/min and the retention time was about 6 minutes. The method is selective to Cefotaxime Sodium and able to resolve the drug peak from formulation excipients. The system suitability with retention time was (Mean + %CV) 8.600 + 0.186.The calibration curve was linear over the concentration range of 1-20µg/ml (r2 = 0.999). The proposed method is accurate and precise (Intra day and Inter day variation, RSD were 0.55-1.67) and linear within the desired range. The LOD and LOQ was detected as 0.0187µg/ml and 0.043µg/ml respectively with r2 = 0.9997. The accuracy result of seventy percent drug (80%) was 99.87%, hundred percent (100%) was 99.93%, and one thirty percent (120%) was 100.18%. Therefore, this method could be used as a more convenient and efficient option for the analysis of Cefotaxime Sodium in raw material and Parentral dosage form.

Keywords:    Third    generation    cephalosporin;    Cefotaxime Sodium;    Method    validation;    HPLC    method determination; Quantitative analysis

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Author Biographies

RY Shah, Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

 
RK Jat, Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

Institute of Pharmacy, JJT University Jhunjhunu (Rajasthan), India

 
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How to Cite
1.
Shah R, Jat R. REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE QUNTIFICATION OF CEFOTAXIME SODIUM IN PHARMACEUTICAL DOSAGE FORM. JDDT [Internet]. 15May2015 [cited 29Sep.2020];5(3):96-00. Available from: http://www.jddtonline.info/index.php/jddt/article/view/1154